2023
A CUG-initiated CATSPERθ functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking
Huang X, Miyata H, Wang H, Mori G, Iida-Norita R, Ikawa M, Percudani R, Chung J. A CUG-initiated CATSPERθ functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2304409120. PMID: 37725640, PMCID: PMC10523455, DOI: 10.1073/pnas.2304409120.Peer-Reviewed Original ResearchConceptsChannel assemblySperm flagellaTransmembrane domain-containing proteinsSperm tail formationDomain-containing proteinsCatSper channel complexMale fertilityFlagellar traffickingMacromolecular complexesTail formationPhysiological roleSuccessful fertilizationCalcium signalingCatSper channelsFlagellaGenetic abrogationChannel complexNormal expressionDimer formationSpermatid cellsProteinCheckpointHyperactivated motilityAssemblyPotential role
2022
Selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis
Dou Q, Turanov AA, Mariotti M, Hwang JY, Wang H, Lee SG, Paulo JA, Yim SH, Gygi SP, Chung JJ, Gladyshev VN. Selenoprotein TXNRD3 supports male fertility via the redox regulation of spermatogenesis. Journal Of Biological Chemistry 2022, 298: 102183. PMID: 35753352, PMCID: PMC9352919, DOI: 10.1016/j.jbc.2022.102183.Peer-Reviewed Original ResearchConceptsThioredoxin/glutathione reductaseThiol redox controlSperm quality controlThiol redox statusGene duplicationThiol oxidoreductasesRedox regulationProteomic analysisRedox controlGross phenotypeThioredoxin reductaseSperm maturationMovement phenotypesSperm cellsRedox statusMale reproductionKnockout animalsMale fertilityGlutathione reductaseCritical roleReductasePhenotypeTXNRD3Knockout miceSpermatogenesis3D structure and in situ arrangements of CatSper channel in the sperm flagellum
Zhao Y, Wang H, Wiesehoefer C, Shah NB, Reetz E, Hwang JY, Huang X, Wang TE, Lishko PV, Davies KM, Wennemuth G, Nicastro D, Chung JJ. 3D structure and in situ arrangements of CatSper channel in the sperm flagellum. Nature Communications 2022, 13: 3439. PMID: 35715406, PMCID: PMC9205950, DOI: 10.1038/s41467-022-31050-8.Peer-Reviewed Original ResearchConceptsCatSper complexSperm flagellaCryo-electron tomographyHigher-order organizationMammalian spermIntracellular domainStructural basisExtracellular domainTetrameric channelChannel CatSperSuccessful fertilizationFlagellar movementCatSper channelsChannel poreSitu mapSitu arrangementHuman sperm flagellumPrimary Ca2CatSperDimer formationFlagellaCentral roleSpermAtomic modelSperm motilityRedox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice
Wang H, Dou Q, Jeong KJ, Choi J, Gladyshev VN, Chung JJ. Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice. Journal Of Biological Chemistry 2022, 298: 102077. PMID: 35643315, PMCID: PMC9218152, DOI: 10.1016/j.jbc.2022.102077.Peer-Reviewed Original ResearchConceptsRedox regulationRedox homeostasisMolecular mechanismsSperm maturationMitochondrial sheath formationCell imaging analysisThioredoxin glutathione reductaseSperm developmentMammalian spermatozoaEpididymal transitMitochondrial ultrastructureMitochondrial activityRedox statusMale fertilityCapacitationHomeostasisMaturationRegulationMotilityFamily membersFertilizationSperm morphologySperm motilityMitochondriaBioenergeticsC2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane
Hwang JY, Wang H, Lu Y, Ikawa M, Chung JJ. C2cd6-encoded CatSperτ targets sperm calcium channel to Ca2+ signaling domains in the flagellar membrane. Cell Reports 2022, 38: 110226. PMID: 34998468, PMCID: PMC8857959, DOI: 10.1016/j.celrep.2021.110226.Peer-Reviewed Original ResearchConceptsFlagellar membraneFlagellar membrane targetingC2 domain proteinsCatSper channelsCatSper channel complexMammalian sperm cellsSperm hyperactivated motilityMale fertilityMembrane targetingTrafficking machineryDomain proteinsLoss of functionHyperactivated motilitySpatiotemporal CaChannel assemblyMolecular mechanismsSperm cellsChannel complexFlagellaMotilityC2CD6MembraneCalcium channelsMachineryProtein
2021
Mitochondria–cytoskeleton interactions in mammalian sperm revealed by cryoelectron tomography
Wang H, Chung JJ. Mitochondria–cytoskeleton interactions in mammalian sperm revealed by cryoelectron tomography. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2118020118. PMID: 34819381, PMCID: PMC8640839, DOI: 10.1073/pnas.2118020118.Commentaries, Editorials and Letters
2020
Sperm ion channels and transporters in male fertility and infertility
Wang H, McGoldrick LL, Chung JJ. Sperm ion channels and transporters in male fertility and infertility. Nature Reviews Urology 2020, 18: 46-66. PMID: 33214707, PMCID: PMC7852504, DOI: 10.1038/s41585-020-00390-9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsSperm ion channelsIon channelsSperm cellsMammalian sperm cellsIon signalingDynamic regulationDefective sperm functionFemale reproductive tractChannel CatSperHuman geneticsMembrane receptorsMale fertilityHealth careAttractive targetDirect electrophysiological recordingsOverall health careHuman infertilityReproductive health careSperm functionPrincipal Ca2Development of contraceptivesSperm activityGene variantsReproductive tractGenetic abnormalities
2017
Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice
Wang H, Chang M, Peng T, Yang Y, Li N, Luo T, Cheng Y, Zhou M, Zeng X, Zheng L. Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice. Cellular Physiology And Biochemistry 2017, 42: 44-54. PMID: 28554186, DOI: 10.1159/000477113.Peer-Reviewed Original ResearchConceptsAdult male C57BL/6 miceMale C57BL/6 miceImpair sperm functionAcute cadmium exposureSperm functionSperm-specific Ca2C57BL/6 miceReproductive toxicityCadmium exposureWestern blottingDifferent dosesSperm dysfunctionExpression levelsEnvironmental heavy metalsToxic effectsMiceEndocrine disruptorsCatSperSperm motilityMouse spermatozoaSpecific roleToxicityExposureMotilityDysfunction
2016
Bisphenol A Impairs Mature Sperm Functions by a CatSper-Relevant Mechanism
Wang H, Liu M, Li N, Luo T, Zheng L, Zeng X. Bisphenol A Impairs Mature Sperm Functions by a CatSper-Relevant Mechanism. Toxicological Sciences 2016, 152: 145-154. PMID: 27125968, DOI: 10.1093/toxsci/kfw070.Peer-Reviewed Original ResearchConceptsToxicity of BPANormal mouse spermSperm functionMature sperm functionVivo administrationEndocrine-disrupting chemicalsWestern blotDifferent dosesSignificant decreaseSperm total motilitySignificant reductionBisphenol AMiceSperm motilityMouse spermMouse spermatozoaMotilityCatSperTotal motilityToxicityMatrine compromises mouse sperm functions by a [Ca2+]i-related mechanism
Luo T, Zou Q, He Y, Wang H, Wang T, Liu M, Chen Y, Wang B. Matrine compromises mouse sperm functions by a [Ca2+]i-related mechanism. Reproductive Toxicology 2016, 60: 69-75. PMID: 26867864, DOI: 10.1016/j.reprotox.2016.02.003.Peer-Reviewed Original ResearchConceptsMouse sperm functionSperm functionDaily dosesIntraperitoneal injectionMale miceSperm countTestis weightReproductive toxicityMiceChinese medicineCatSper channelsMatrineProgressive motilityAcrosome reactionKey regulatorMouse spermTotal motilitySperm viabilityGene expressionTestis sizeMotilitySpermDoses
2015
Matrine Inhibits Mouse Sperm Function by Reducing Sperm [Ca2+]i and Phospho-ERK1/2
Luo T, Zou Q, He Y, Wang H, Li N, Zeng X. Matrine Inhibits Mouse Sperm Function by Reducing Sperm [Ca2+]i and Phospho-ERK1/2. Cellular Physiology And Biochemistry 2015, 35: 374-385. PMID: 25591778, DOI: 10.1159/000369703.Peer-Reviewed Original ResearchMeSH KeywordsAcrosome ReactionAlkaloidsAniline CompoundsAnimalsCalcimycinCalciumFemaleFertilization in VitroMaleMatrinesMiceMice, Inbred C57BLMicroscopy, FluorescenceMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Patch-Clamp TechniquesPhosphorylationPotassiumQuinolizinesSperm MotilitySpermatozoaXanthenesConceptsMouse sperm functionIntracellular calcium concentrationSperm functionCauda epididymal spermMouse spermPhosphorylation of ERK1/2Acrosome reaction rateEpididymal spermMouse cauda epididymal spermFertilization abilityPharmacological effectsPotassium currentP-ERK1/2Phospho-ERK1/2Calcium concentrationChinese medicineKinase 1/2Male reproductionSignificant reductionMatrineMature mouse spermProgressive motilityAcrosome reactionMotilityTotal motility