2012
Nature and function of insulator protein binding sites in the Drosophila genome
Schwartz Y, Linder-Basso D, Kharchenko P, Tolstorukov M, Kim M, Li H, Gorchakov A, Minoda A, Shanower G, Alekseyenko A, Riddle N, Jung Y, Gu T, Plachetka A, Elgin S, Kuroda M, Park P, Savitsky M, Karpen G, Pirrotta V. Nature and function of insulator protein binding sites in the Drosophila genome. Genome Research 2012, 22: 2188-2198. PMID: 22767387, PMCID: PMC3483548, DOI: 10.1101/gr.138156.112.Peer-Reviewed Original ResearchConceptsInsulator proteinsQuantitative genome-wide analysisInsulator elementsGenome-wide analysisEnhancer-blocking assaysRepressive histone methylationDrosophila chromatinDrosophila genomeEukaryotic genomesCombinatorial bindingH3K27me3 marksHistone methylationRNAi knockdownAdjacent genesGenome expressionFunctional characterizationAssociated proteinsGenomeCultured cellsProteinSpecific regulationGenesMajor alterationsDifferent classesChromatin
2011
Insulators, Not Polycomb Response Elements, Are Required for Long-Range Interactions between Polycomb Targets in Drosophila melanogaster
Li HB, Müller M, Bahechar IA, Kyrchanova O, Ohno K, Georgiev P, Pirrotta V. Insulators, Not Polycomb Response Elements, Are Required for Long-Range Interactions between Polycomb Targets in Drosophila melanogaster. Molecular And Cellular Biology 2011, 31: 616-625. PMID: 21135119, PMCID: PMC3028641, DOI: 10.1128/mcb.00849-10.Peer-Reviewed Original ResearchConceptsPolycomb response elementsPcG targetsPcG complexesResponse elementChromosome conformation capture methodsPolycomb group complexesFab-7 elementHigher-order organizationPolycomb targetsDrosophila melanogasterEnhanced repressionInsulator activityRepressive activityTrans interactionsRegulatory elementsPhysical colocalizationFunctional assaysGroup complexesCapture methodComplexesFluorescence imagingPolycombDrosophilaMelanogasterMajor determinant