2013
Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies
Li HB, Ohno K, Gui H, Pirrotta V. Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies. PLOS Genetics 2013, 9: e1003436. PMID: 23637616, PMCID: PMC3630138, DOI: 10.1371/journal.pgen.1003436.Peer-Reviewed Original ResearchMeSH KeywordsCell NucleusDNA-Binding ProteinsDrosophila ProteinsHumansPolycomb Repressive Complex 1Polycomb-Group ProteinsResponse ElementsConceptsPolycomb response elementsPolycomb target genesPolycomb bodiesTranscription factoriesTarget genesSpecialized transcription factoriesAbsence of CTCFInsulator protein CTCFInsulator proteinsPolycomb proteinsPolycomb groupProtein CTCFActive genesChromosomal contextCellular memoryEnhancer specificityGenomic clustersStable associationResponse elementGenesDifferent nuclear structuresTrithoraxCTCFActive stateCritical role
2012
Nature and function of insulator protein binding sites in the Drosophila genome
Schwartz Y, Linder-Basso D, Kharchenko P, Tolstorukov M, Kim M, Li H, Gorchakov A, Minoda A, Shanower G, Alekseyenko A, Riddle N, Jung Y, Gu T, Plachetka A, Elgin S, Kuroda M, Park P, Savitsky M, Karpen G, Pirrotta V. Nature and function of insulator protein binding sites in the Drosophila genome. Genome Research 2012, 22: 2188-2198. PMID: 22767387, PMCID: PMC3483548, DOI: 10.1101/gr.138156.112.Peer-Reviewed Original ResearchConceptsInsulator proteinsQuantitative genome-wide analysisInsulator elementsGenome-wide analysisEnhancer-blocking assaysRepressive histone methylationDrosophila chromatinDrosophila genomeEukaryotic genomesCombinatorial bindingH3K27me3 marksHistone methylationRNAi knockdownAdjacent genesGenome expressionFunctional characterizationAssociated proteinsGenomeCultured cellsProteinSpecific regulationGenesMajor alterationsDifferent classesChromatin
2011
A view of nuclear Polycomb bodies
Pirrotta V, Li HB. A view of nuclear Polycomb bodies. Current Opinion In Genetics & Development 2011, 22: 101-109. PMID: 22178420, PMCID: PMC3329586, DOI: 10.1016/j.gde.2011.11.004.Peer-Reviewed Original Research