2022
Diverging regulation of Bach2 protein and RNA expression determine cell fate in early B cell response
Hu Q, Xu T, Zhang M, Zhang H, Liu Y, Li H, Chen C, Zheng J, Zhang Z, Li F, Shen N, Zhang W, Melnick A, Huang C. Diverging regulation of Bach2 protein and RNA expression determine cell fate in early B cell response. Cell Reports 2022, 40: 111035. PMID: 35793628, PMCID: PMC9550188, DOI: 10.1016/j.celrep.2022.111035.Peer-Reviewed Original ResearchConceptsBach2 proteinCell fateActivated B cellsMemory B cellsB cellsCell fate choiceDetermines cell fateCell fate outcomesRapamycin complex 1B cell fateEffector cellsGerminal centre B cell fatePivotal transcription factorB cell receptor affinityEarly B cell responsesTranscription factorsDependent translationB cell responsesPrimary humoral responseGC fateMechanistic targetHumoral responseProteinPlasma cellsDifferential dynamics
2016
Chromosome Conformation Capture in Drosophila
Li HB. Chromosome Conformation Capture in Drosophila. Methods In Molecular Biology 2016, 1480: 207-212. PMID: 27659987, DOI: 10.1007/978-1-4939-6380-5_18.Peer-Reviewed Original ResearchConceptsDNA-DNA interactionsChromosome conformation capture techniquesChromosome conformation captureInsulator proteinsConformation captureThree-dimensional structureChromatin fibersTemporal regulationRegulatory proteinsGene expressionDrosophilaChromatinProteinCapture techniquesImportant roleGenesPCGNucleusRegulationExpressionInteraction
2012
Nature and function of insulator protein binding sites in the Drosophila genome
Schwartz Y, Linder-Basso D, Kharchenko P, Tolstorukov M, Kim M, Li H, Gorchakov A, Minoda A, Shanower G, Alekseyenko A, Riddle N, Jung Y, Gu T, Plachetka A, Elgin S, Kuroda M, Park P, Savitsky M, Karpen G, Pirrotta V. Nature and function of insulator protein binding sites in the Drosophila genome. Genome Research 2012, 22: 2188-2198. PMID: 22767387, PMCID: PMC3483548, DOI: 10.1101/gr.138156.112.Peer-Reviewed Original ResearchConceptsInsulator proteinsQuantitative genome-wide analysisInsulator elementsGenome-wide analysisEnhancer-blocking assaysRepressive histone methylationDrosophila chromatinDrosophila genomeEukaryotic genomesCombinatorial bindingH3K27me3 marksHistone methylationRNAi knockdownAdjacent genesGenome expressionFunctional characterizationAssociated proteinsGenomeCultured cellsProteinSpecific regulationGenesMajor alterationsDifferent classesChromatin