2013
Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies
Li HB, Ohno K, Gui H, Pirrotta V. Insulators Target Active Genes to Transcription Factories and Polycomb-Repressed Genes to Polycomb Bodies. PLOS Genetics 2013, 9: e1003436. PMID: 23637616, PMCID: PMC3630138, DOI: 10.1371/journal.pgen.1003436.Peer-Reviewed Original ResearchConceptsPolycomb response elementsPolycomb target genesPolycomb bodiesTranscription factoriesTarget genesSpecialized transcription factoriesAbsence of CTCFInsulator protein CTCFInsulator proteinsPolycomb proteinsPolycomb groupProtein CTCFActive genesChromosomal contextCellular memoryEnhancer specificityGenomic clustersStable associationResponse elementGenesDifferent nuclear structuresTrithoraxCTCFActive stateCritical role
2011
Insulators, Not Polycomb Response Elements, Are Required for Long-Range Interactions between Polycomb Targets in Drosophila melanogaster
Li HB, Müller M, Bahechar IA, Kyrchanova O, Ohno K, Georgiev P, Pirrotta V. Insulators, Not Polycomb Response Elements, Are Required for Long-Range Interactions between Polycomb Targets in Drosophila melanogaster. Molecular And Cellular Biology 2011, 31: 616-625. PMID: 21135119, PMCID: PMC3028641, DOI: 10.1128/mcb.00849-10.Peer-Reviewed Original ResearchConceptsPolycomb response elementsPcG targetsPcG complexesResponse elementChromosome conformation capture methodsPolycomb group complexesFab-7 elementHigher-order organizationPolycomb targetsDrosophila melanogasterEnhanced repressionInsulator activityRepressive activityTrans interactionsRegulatory elementsPhysical colocalizationFunctional assaysGroup complexesCapture methodComplexesFluorescence imagingPolycombDrosophilaMelanogasterMajor determinant