2021
VPS13D bridges the ER to mitochondria and peroxisomes via Miro
Guillén-Samander A, Leonzino M, Hanna MG, Tang N, Shen H, De Camilli P. VPS13D bridges the ER to mitochondria and peroxisomes via Miro. Journal Of Cell Biology 2021, 220: e202010004. PMID: 33891013, PMCID: PMC8077184, DOI: 10.1083/jcb.202010004.Peer-Reviewed Original ResearchConceptsLipid transport proteinsHigher eukaryotesER-mitochondriaSecretory pathwayAccessory factorsMitochondrial dynamicsDisease pathogenesisTransport proteinsParkin substratesLipid transferSplice variantsParkinson's disease pathogenesisVps13Lipid supplyMitochondriaMiroVPS13DERMESYeastMost lipidsTransport domainEukaryotesGem1MetazoansER
2017
A genetically encoded tool for manipulation of NADP+/NADPH in living cells
Cracan V, Titov DV, Shen H, Grabarek Z, Mootha VK. A genetically encoded tool for manipulation of NADP+/NADPH in living cells. Nature Chemical Biology 2017, 13: 1088-1095. PMID: 28805804, PMCID: PMC5605434, DOI: 10.1038/nchembio.2454.Peer-Reviewed Original Research
2014
Coupling between endocytosis and sphingosine kinase 1 recruitment
Shen H, Giordano F, Wu Y, Chan J, Zhu C, Milosevic I, Wu X, Yao K, Chen B, Baumgart T, Sieburth D, De Camilli P. Coupling between endocytosis and sphingosine kinase 1 recruitment. Nature Cell Biology 2014, 16: 652-662. PMID: 24929359, PMCID: PMC4230894, DOI: 10.1038/ncb2987.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaenorhabditisCell MembraneCells, CulturedChlorocebus aethiopsCholesterolCOS CellsEndocytosisFluorescent Antibody TechniqueHEK293 CellsHeLa CellsHumansMiceModels, MolecularMutationPhosphotransferases (Alcohol Group Acceptor)Protein BindingProtein Structure, TertiarySequence AnalysisConceptsSphingosine kinase 1Hydrophobic patchN-BAR proteinsEndocytic membrane trafficExo/endocytosisPlasma membrane resultsMembrane trafficEndocytic intermediatesFunction mutantsMembrane recruitmentEndocytic membranesCellular compartmentsIntercellular signalingRecycling defectsSphingosine phosphorylationKinase 1Genetic studiesFunctional linkTubular invaginationsSphingolipid metabolismLipid bilayersEndocytosisEnzyme surfaceMembrane resultsRecruitment