2013
Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors
Park RJ, Shen H, Liu L, Liu X, Ferguson SM, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. Journal Of Cell Science 2013, 126: 5305-5312. PMID: 24046449, PMCID: PMC3828596, DOI: 10.1242/jcs.138578.Peer-Reviewed Original ResearchConceptsDynamin 1Dynamin inhibitorTKO cellsPeripheral membrane rufflingDouble knockoutDynamin 3 geneClathrin-mediated endocytosisTriple-knockout cellsDynamin-dependent processFluid-phase endocytosisDyngo-4aMembrane fissionMembrane rufflingDKO cellsDynamin 2Knockout cellsLow-level expressionCell physiologyDynaminEndocytosisTarget effectsDKO fibroblastsGenesCellsKO fibroblasts
2011
Recruitment of Endophilin to Clathrin-Coated Pit Necks Is Required for Efficient Vesicle Uncoating after Fission
Milosevic I, Giovedi S, Lou X, Raimondi A, Collesi C, Shen H, Paradise S, O'Toole E, Ferguson S, Cremona O, De Camilli P. Recruitment of Endophilin to Clathrin-Coated Pit Necks Is Required for Efficient Vesicle Uncoating after Fission. Neuron 2011, 72: 587-601. PMID: 22099461, PMCID: PMC3258500, DOI: 10.1016/j.neuron.2011.08.029.Peer-Reviewed Original Research
2009
Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits
Ferguson S, Raimondi A, Paradise S, Shen H, Mesaki K, Ferguson A, Destaing O, Ko G, Takasaki J, Cremona O, Toole E, De Camilli P. Coordinated Actions of Actin and BAR Proteins Upstream of Dynamin at Endocytic Clathrin-Coated Pits. Developmental Cell 2009, 17: 811-822. PMID: 20059951, PMCID: PMC2861561, DOI: 10.1016/j.devcel.2009.11.005.Peer-Reviewed Original ResearchConceptsEndocytic roleEndocytic clathrin-coated pitsBAR domain proteinsSense membrane curvatureClathrin-coated pitsWild-type cellsGTPase dynaminEndocytic intermediatesHigher eukaryotesDomain proteinsMembrane fissionActin dynamicsNumerous proteinsDynaminMembrane curvatureProtein upstreamTubular neckConcerted actionProteinActinCoordinated actionKey playersFunctional relationshipCellsSequence of events