Prostate-specific antigen in amniotic fluid of normal and abnormal pregnancies
Melegos D, Yu H, Allen L, Diamandis E. Prostate-specific antigen in amniotic fluid of normal and abnormal pregnancies. Clinical Biochemistry 1996, 29: 555-562. PMID: 8939403, DOI: 10.1016/s0009-9120(96)00093-8.Peer-Reviewed Original ResearchConceptsProstate-specific antigenSerum PSA concentrationAmniotic fluidPregnant womenFetal abnormalitiesPSA concentrationNonpregnant womenGestational ageHigher serum PSA concentrationsSerum prostate-specific antigenGestational week 15Gestational weeks 11Median PSA concentrationGestational weeks 13Gender of fetusesPSA levelsMaternal serumAbnormal pregnanciesMaternal ageRenal disordersLength of abstinenceWeek 13Week 15Diagnostic usefulnessTime-resolved immunofluorometric procedureProstaglandin D2 synthase: a component of human amniotic fluid and its association with fetal abnormalities.
Melegos D, Yu H, Diamandis E. Prostaglandin D2 synthase: a component of human amniotic fluid and its association with fetal abnormalities. Clinical Chemistry 1996, 42: 1042-1050. PMID: 8674187, DOI: 10.1093/clinchem/42.7.1042.Peer-Reviewed Original ResearchConceptsProstate-specific antigenAmniotic fluidHuman amniotic fluidGestational week 12Western blot analysisAbnormal pregnanciesPGD2 synthaseStrong immunoreactive bandWeek 12Monoclonal anti-PSA antibodiesRenal abnormalitiesProstaglandin D2 synthaseTrisomic fetusesPGD2 productionFetal abnormalitiesAnti-PSA antibodiesImmunoreactive bandsBlot analysisSemiquantitative analysisFetusesAbnormalitiesPolyclonal antibodiesImmunoreactive speciesAntibodiesAmino acid sequence analysis