2021
YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion
Feliciano D, Ott CM, Espinosa-Medina I, Weigel AV, Benedetti L, Milano KM, Tang Z, Lee T, Kliman HJ, Guller SM, Lippincott-Schwartz J. YAP1 nuclear efflux and transcriptional reprograming follow membrane diminution upon VSV-G-induced cell fusion. Nature Communications 2021, 12: 4502. PMID: 34301937, PMCID: PMC8302681, DOI: 10.1038/s41467-021-24708-2.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAMP-Activated Protein KinasesAnimalsBiological TransportCell FusionCell LineCell Line, TumorCell MembraneCell NucleusCells, CulturedGiant CellsHEK293 CellsHumansMembrane GlycoproteinsMiceRNA-SeqSignal TransductionTranscription FactorsTranscription, GeneticViral Envelope ProteinsYAP-Signaling ProteinsConceptsCell cycle arrestCell fusionNew cellular statesPlasma membrane surface areaRNA-seq analysisCell fusion systemTranscriptional programsNutrient stressCellular statesTranscriptional changesNuclear effluxCytoplasmic glucoseExtrinsic cuesUndifferentiated cellsGlucose transporterFused cellsMechanistic insightsMembrane surface areaNew functionsEndocytosisYAP1 inhibitionEnergetic stateSyncytiaCellsVSV
2008
Timing of Fetal Meconium Absorption by Amnionic Macrophages
Funai E, Labowsky A, Drewes C, Kliman H. Timing of Fetal Meconium Absorption by Amnionic Macrophages. American Journal Of Perinatology 2008, 26: 093-097. PMID: 19031358, DOI: 10.1055/s-0028-1103028.Peer-Reviewed Original Research
1996
Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube
Keltz M, Attar E, Buradagunta S, Olive D, Kliman H, Arici A. Modulation of leukemia inhibitory factor gene expression and protein biosynthesis in the human fallopian tube. American Journal Of Obstetrics And Gynecology 1996, 175: 1611-1619. PMID: 8987949, DOI: 10.1016/s0002-9378(96)70114-x.Peer-Reviewed Original ResearchMeSH KeywordsAdultBlood Physiological PhenomenaCells, CulturedCulture MediaCytokinesEpitheliumFallopian TubesFemaleGene Expression RegulationGrowth InhibitorsGrowth SubstancesHormonesHumansInterleukin-6Leukemia Inhibitory FactorLymphokinesMiddle AgedPregnancyPregnancy, EctopicRNA, MessengerStromal CellsConceptsLeukemia inhibitory factor expressionLeukemia inhibitory factorFallopian tubeInhibitory factorStromal cellsEctopic pregnancyStromal cell culturesGrowth factorMessenger ribonucleic acidLeukemia inhibitory factor gene expressionEctopic implantationFactor expressionTubal ectopic pregnancyEpithelial cellsInterleukin-1 alphaHuman fallopian tubePlatelet-derived growth factorMessenger ribonucleic acid levelsRibonucleic acid levelsFactor gene expressionTubal mucosaMenstrual cycleTNF-alphaTumor necrosisCommon siteThe effect of leukemia inhibitory factor (LIF) on trophoblast differentiation: a potential role in human implantation
Nachtigall MJ, Kliman HJ, Feinberg RF, Olive DL, Engin O, Arici A. The effect of leukemia inhibitory factor (LIF) on trophoblast differentiation: a potential role in human implantation. The Journal Of Clinical Endocrinology & Metabolism 1996, 81: 801-806. PMID: 8636307, DOI: 10.1210/jcem.81.2.8636307.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, NorthernCell DifferentiationCells, CulturedChorionic GonadotropinChorionic Gonadotropin, beta Subunit, HumanEmbryo ImplantationFemaleFibronectinsGene ExpressionGrowth InhibitorsHumansInterleukin-6Leukemia Inhibitory FactorLymphokinesPregnancyProgesteroneRNA, MessengerTrophoblastsConceptsLeukemia inhibitory factorHuman cytotrophoblastsMenstrual cycle-dependent mannerInhibitory factorOncofetal fibronectinEffects of LIFHuman implantationHuman embryonic implantationBeta-HCG mRNAOncofetal fibronectin mRNAMessenger RNA expressionExtravillous phenotypeBeta-hCGMenstrual cycleHuman endometriumNormal implantationEmbryonic implantationProgesterone productionHCG mRNATerm placentaBiochemical markersCultured trophoblastsHuman CGDay 19Steroidogenic activityEndocrinology and paracrinology
Arici A, Tazuke SI, Attar E, Kliman HJ, Olive DL. Endocrinology and paracrinology. Molecular Human Reproduction 1996, 2: 40-45. PMID: 9238656, DOI: 10.1093/molehr/2.1.40.Peer-Reviewed Original ResearchConceptsIL-8 mRNAIL-1 alphaEnzyme-linked immunosorbent assayIL-8 concentrationsPathogenesis of endometriosisInterleukin-8IL-8 proteinPeritoneal fluidMesothelial cellsPg/Human recombinant IL-1 alphaRecombinant IL-1 alphaNeutrophil chemotactic activityMinimal-mild endometriosisPeritoneal mesothelial cellsInterleukin-8 expressionCultured mesothelial cellsPotent angiogenic agentTNF-alpha treatmentModerate-severe endometriosisHuman mesothelial cellsNon-treated cellsControl patientsTime-dependent mannerTNF-alpha
1995
First-trimester endocervical irrigation: feasibility of obtaining trophoblast cells for prenatal diagnosis.
Bahado-Singh RO, Kliman H, Feng TY, Hobbins J, Copel JA, Mahoney MJ. First-trimester endocervical irrigation: feasibility of obtaining trophoblast cells for prenatal diagnosis. Obstetrics And Gynecology 1995, 85: 461-4. PMID: 7862393, DOI: 10.1016/0029-7844(94)00416-b.Peer-Reviewed Original ResearchConceptsTrophoblast cellsPrenatal diagnosisInternal cervical osElective pregnancy terminationFirst trimester diagnosisPlacental pathologistPregnant patientsWeeks' gestationCervical osNormal salinePregnancy terminationUltrasound guidanceAdditional casesPlastic catheterDiagnosisFirst-trimester prenatal diagnosisBrief durationGestationHCG antibodySignificant percentageFurther testingCellsLight microscopyTechnical simplicityPatients
1994
Transforming growth factor-beta stimulates trophoblast oncofetal fibronectin synthesis in vitro: implications for trophoblast implantation in vivo
Feinberg RF, Kliman HJ, Wang CL. Transforming growth factor-beta stimulates trophoblast oncofetal fibronectin synthesis in vitro: implications for trophoblast implantation in vivo. The Journal Of Clinical Endocrinology & Metabolism 1994, 78: 1241-1248. PMID: 8175984, DOI: 10.1210/jcem.78.5.8175984.Peer-Reviewed Original ResearchConceptsTGF beta 1TGF betaBeta 1Ability of trophoblastsAnti-TGF betaSevere alloimmune thrombocytopeniaThird trimester placentasPlatelet-poor serumDose-response analysisPregnancy tissueCord serumAlloimmune thrombocytopeniaCytokine presentUterine deciduaExtracellular matrixVillous syncytiotrophoblastTrimester placentaNormal serumHuman cytotrophoblastsGrowth factor-beta stimulatesOncofetal fibronectinNormal morphological differentiationCulture secreteStimulatory responseThrombocytopenic serum
1991
Is oncofetal fibronectin a trophoblast glue for human implantation?
Feinberg RF, Kliman HJ, Lockwood CJ. Is oncofetal fibronectin a trophoblast glue for human implantation? American Journal Of Pathology 1991, 138: 537-43. PMID: 2000933, PMCID: PMC1886265.Peer-Reviewed Original Research
1990
Human trophoblast-endometrial interactions in an in vitro suspension culture system
Kliman H, Feinberg R, Haimowitz J. Human trophoblast-endometrial interactions in an in vitro suspension culture system. Placenta 1990, 11: 349-367. PMID: 2235916, DOI: 10.1016/s0143-4004(05)80226-7.Peer-Reviewed Original Research
1989
Plasminogen activator inhibitor types 1 and 2 in human trophoblasts. PAI-1 is an immunocytochemical marker of invading trophoblasts.
Feinberg RF, Kao LC, Haimowitz JE, Queenan JT, Wun TC, Strauss JF, Kliman HJ. Plasminogen activator inhibitor types 1 and 2 in human trophoblasts. PAI-1 is an immunocytochemical marker of invading trophoblasts. Laboratory Investigation 1989, 61: 20-6. PMID: 2473276.Peer-Reviewed Original ResearchConceptsPAI-2Plasminogen activator inhibitorSpecific plasminogen activator inhibitorPAI-1Villous syncytiotrophoblastHuman trophoblastsCytoplasmic localizationPlasminogen activatorRegulated productionPAI expressionCytoplasmic stainingCell surface stainingCultured cytotrophoblastsProminent cytoplasmic stainingIntervillous blood flowPlasminogen activator inhibitor type 1Activator inhibitor type 1Inhibitor type 1First trimesterActivatorProteolytic enzymesVascular remodelingVillous cytotrophoblastsBlood flowImmunohistochemical analysisThe human trophoblast: homotypic and heterotypic cell-cell interactions.
Kliman HJ, Coutifaris C, Babalola GO, Soto EA, Kao LC, Queenan JT, Feinberg RF, Strauss JF. The human trophoblast: homotypic and heterotypic cell-cell interactions. Progress In Clinical And Biological Research 1989, 294: 425-34. PMID: 2726976.Peer-Reviewed Original Research
1988
The human villous cytotrophoblast: Interactions with extracellular matrix proteins, endocrine function, and cytoplasmic differentiation in the absence of syncytium formation
Kao L, Caltabiano S, Wu S, Strauss J, Kliman H. The human villous cytotrophoblast: Interactions with extracellular matrix proteins, endocrine function, and cytoplasmic differentiation in the absence of syncytium formation. Developmental Biology 1988, 130: 693-702. PMID: 2848742, DOI: 10.1016/0012-1606(88)90361-2.Peer-Reviewed Original ResearchBiosynthesis and turnover of a 34‐kDa protein growth factor in human cytotrophoblasts
ROY‐CHOUDHURY S, SEN‐MAJUMDAR A, MURTHY U, MISHRA V, KLIMAN H, NESTLER J, STRAUSS J, Manjusri D. Biosynthesis and turnover of a 34‐kDa protein growth factor in human cytotrophoblasts. The FEBS Journal 1988, 172: 777-783. PMID: 3350024, DOI: 10.1111/j.1432-1033.1988.tb13957.x.Peer-Reviewed Original ResearchConceptsProtein growth factorsWestern blot analysisMolecular massPolypeptide molecular massPartial amino acid sequenceAmino acid sequenceBlot analysisGrowth factorMetabolic labeling experimentsPulse-chase experimentsCellular mRNAsVitro translationAcid sequenceReceptor binding specificityBinding specificityIntact cellsLow molecular massBiosynthesisCultured cytotrophoblastsProteinMultinuclear syncytiotrophoblastHuman placentaLabeling experimentsHuman cytotrophoblastsCells
1987
HLA antigen expression and induction by γ-interferon in cultured human trophoblasts
Feinman M, Kliman H, Main E. HLA antigen expression and induction by γ-interferon in cultured human trophoblasts. American Journal Of Obstetrics And Gynecology 1987, 157: 1429-1434. PMID: 3122576, DOI: 10.1016/s0002-9378(87)80238-7.Peer-Reviewed Original ResearchConceptsHuman trophoblastsHLA antigen expressionHLA class I heavy chainsClass II antigensClass I HLACultured human trophoblastsHLA heavy chainsPregnancy complicationsHLA antigensClass I heavy chainsII antigensAntigen expressionHeavy chainI HLAΓ-interferonImmunocytochemical studyAntigenMonoclonal antibodiesClass ICellular stainingTime courseTrophoblastCytotrophoblastsExpressionGene expression
1986
8-Bromo-3′5′ -Adenosine Monophosphate Stimulates the Endocrine Activity of Human Cytotrophoblasts in Culture*
FEINMAN M, KLIMAN H, CALTABIANO S, STRAUSS J. 8-Bromo-3′5′ -Adenosine Monophosphate Stimulates the Endocrine Activity of Human Cytotrophoblasts in Culture*. The Journal Of Clinical Endocrinology & Metabolism 1986, 63: 1211-1217. PMID: 3531221, DOI: 10.1210/jcem-63-5-1211.Peer-Reviewed Original ResearchGonadotropins and Cyclic Adenosine 3′,5′-Monophosphate (cAMP) Alter the Morphology of Cultured Human Granulosa Cells
Soto E, Kliman H, Strauss J, Paavola L. Gonadotropins and Cyclic Adenosine 3′,5′-Monophosphate (cAMP) Alter the Morphology of Cultured Human Granulosa Cells. Biology Of Reproduction 1986, 34: 559-569. PMID: 3008869, DOI: 10.1095/biolreprod34.3.559.Peer-Reviewed Original Research