2019
Mitochondrial thioredoxin-2 maintains HCN4 expression and prevents oxidative stress-mediated sick sinus syndrome
Yang B, Huang Y, Zhang H, Huang Y, Zhou HJ, Young L, Xiao H, Min W. Mitochondrial thioredoxin-2 maintains HCN4 expression and prevents oxidative stress-mediated sick sinus syndrome. Journal Of Molecular And Cellular Cardiology 2019, 138: 291-303. PMID: 31751569, DOI: 10.1016/j.yjmcc.2019.10.009.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBradycardiaCardiomyopathy, DilatedEnhancer Elements, GeneticHistone DeacetylasesHyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMEF2 Transcription FactorsMice, KnockoutMitochondria, HeartModels, BiologicalOxidative StressPhenotypeProtein BindingReactive Oxygen SpeciesRNA, MessengerSick Sinus SyndromeSinoatrial NodeThioredoxinsConceptsSick sinus syndromeSinus syndromeHistone deacetylase 4Lower heart rateHeart rateHCN4 expressionConduction systemSinoatrial nodeNormal heart rateCardiac conduction systemHistone 3 acetylationMitochondrial oxidative stressSinus bradycardiaCardiac functionLox/SyndromeHeart rhythmMyosin heavy chainHistological analysisMiceDeletion miceOxidative stressWhole heartProtein levelsUnderlying mechanism
2017
The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis
Qiu C, Wang Y, Zhao H, Qin L, Shi Y, Zhu X, Song L, Zhou X, Chen J, Zhou H, Zhang H, Tellides G, Min W, Yu L. The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis. Nature Communications 2017, 8: 15426. PMID: 28569748, PMCID: PMC5461500, DOI: 10.1038/ncomms15426.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriosclerosisCysteine EndopeptidasesDisease ProgressionDNAEndopeptidasesEndothelial CellsEndothelium, VascularGATA2 Transcription FactorHuman Umbilical Vein Endothelial CellsHumansInflammation MediatorsLeukocytesMaleMice, Inbred C57BLMice, KnockoutModels, BiologicalProtein BindingProtein StabilitySumoylationConceptsGraft arteriosclerosisEndothelial activationClinical graft rejectionConsequent endothelial dysfunctionNF-κB activityRole of SENP1Post-translational SUMOylationAllograft failureEndothelial dysfunctionGraft rejectionGraft endotheliumLeukocyte recruitmentVascular remodellingCardiovascular disordersNeointima formationNF-κBClinical researchDiminished inductionEndothelial cellsMajor causeAdhesion moleculesPotential involvementInflammationArteriosclerosisSENP1
2010
SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis
Yu L, Ji W, Zhang H, Renda MJ, He Y, Lin S, Cheng EC, Chen H, Krause DS, Min W. SENP1-mediated GATA1 deSUMOylation is critical for definitive erythropoiesis. Journal Of Experimental Medicine 2010, 207: 1183-1195. PMID: 20457756, PMCID: PMC2882842, DOI: 10.1084/jem.20092215.Peer-Reviewed Original ResearchConceptsSmall ubiquitin-like modifier (SUMO) modificationImportant regulatory mechanismEmbryonic day 13.5Down-regulation correlatesFetal liverCre-loxP systemEmbryonic lethalityProtein functionDefinitive erythropoiesisGene promoterDNA bindingRegulatory mechanismsGene expressionGATA1SENP1Fetal liver cellsProtein analysisDay 13.5Global deletionProteinSubsequent erythropoiesisKnockout miceErythropoiesisLiver cellsDeSUMOylation
2009
AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling2
Wan T, Liu T, Zhang H, Tang S, Min W. AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling2. Journal Of Biological Chemistry 2009, 285: 3750-3757. PMID: 19948740, PMCID: PMC2823516, DOI: 10.1074/jbc.m109.069385.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingADP-Ribosylation Factor 6ADP-Ribosylation FactorsAmino Acid SequenceAnimalsCarrier ProteinsCattleCell LineCells, CulturedChlorocebus aethiopsCOS CellsGTPase-Activating ProteinsGuanylate KinasesHumansImmunoblottingLipopolysaccharidesMembrane GlycoproteinsMiceMice, KnockoutMitogen-Activated Protein KinasesMolecular Sequence DataMyeloid Differentiation Factor 88NF-kappa BPhosphatidylinositol 4,5-DiphosphateProtein BindingReceptors, Interleukin-1Sequence Homology, Amino AcidToll-Like Receptor 4TransfectionConceptsGTPase-activating proteinsArf6 GAPAIP1 functionsNovel GTPase-activating proteinInhibition of ARF6Pleckstrin homologyGAP domainAdaptor proteinSmall GTPaseDisrupts formationPlasma membraneAIP1MAPK pathwayLipid precursorsToll-like receptor 4Arf6NF-kappaBComplex componentsToll-like receptorsProteinRich sitesGTPaseHomologyComplexesCells increases
2008
AIP1 Is Critical in Transducing IRE1-mediated Endoplasmic Reticulum Stress Response*
Luo D, He Y, Zhang H, Yu L, Chen H, Xu Z, Tang S, Urano F, Min W. AIP1 Is Critical in Transducing IRE1-mediated Endoplasmic Reticulum Stress Response*. Journal Of Biological Chemistry 2008, 283: 11905-11912. PMID: 18281285, PMCID: PMC2335342, DOI: 10.1074/jbc.m710557200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCattleDimerizationDNA-Binding ProteinsEndoplasmic ReticulumEndothelial CellsEnzyme ActivationHumansJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase Kinase 5Membrane ProteinsMiceMice, KnockoutProtein BindingProtein Serine-Threonine KinasesProtein Structure, TertiaryRas GTPase-Activating ProteinsRegulatory Factor X Transcription FactorsSignal TransductionTranscription FactorsX-Box Binding Protein 1ConceptsASK1-interacting protein-1
2007
RIP1-mediated AIP1 Phosphorylation at a 14-3-3-binding Site Is Critical for Tumor Necrosis Factor-induced ASK1-JNK/p38 Activation*
Zhang R, Zhang H, Lin Y, Li J, Pober JS, Min W. RIP1-mediated AIP1 Phosphorylation at a 14-3-3-binding Site Is Critical for Tumor Necrosis Factor-induced ASK1-JNK/p38 Activation*. Journal Of Biological Chemistry 2007, 282: 14788-14796. PMID: 17389591, DOI: 10.1074/jbc.m701148200.Peer-Reviewed Original ResearchMeSH Keywords14-3-3 ProteinsAdaptor Proteins, Signal TransducingAmino Acid SubstitutionAnimalsApoptosisCarrier ProteinsCattleCells, CulturedEndothelial CellsEnzyme ActivationGuanylate KinasesHumansMAP Kinase Kinase 4MAP Kinase Kinase Kinase 5MAP Kinase Signaling SystemMultiprotein ComplexesMutation, MissenseP38 Mitogen-Activated Protein KinasesPhosphorylationProtein BindingProtein Processing, Post-TranslationalProteinsReceptor-Interacting Protein Serine-Threonine KinasesTNF Receptor-Associated Factor 2Tumor Necrosis Factor-alphaConceptsJNK/p38 activationP38 activationTRAF2-ASK1ASK1-JNK activationPhospho-specific antibodiesTNF treatmentEndothelial cellsComplex formationGAP domainProtein familyTerminal domainAIP1Novel memberApoptotic signalingTNF signalingRNA knockdownRIP1PhosphorylationProtein 1ASK1-interacting protein-1EC apoptosisTRAF2ASK1Similar kineticsTumor necrosis factor