Structural and Functional Characterization of the Human Protein Kinase ASK1
Bunkoczi G, Salah E, Filippakopoulos P, Fedorov O, Müller S, Sobott F, Parker SA, Zhang H, Min W, Turk BE, Knapp S. Structural and Functional Characterization of the Human Protein Kinase ASK1. Structure 2007, 15: 1215-1226. PMID: 17937911, PMCID: PMC2100151, DOI: 10.1016/j.str.2007.08.011.Peer-Reviewed Original ResearchConceptsApoptosis signal-regulating kinase 1Autophosphorylation sitesSignal-regulated kinases 1ASK1 kinase activityVitro autophosphorylation sitesAnalytical ultracentrifugationHigh-resolution structuresATP-mimetic inhibitorSite-directed mutantsReporter gene assayPhosphorylation motifsPhosphorylation sitesCatalytic domainFunctional characterizationKinase activityRegulatory mechanismsKinase 1Tight dimerMimetic inhibitorsGene assayEssential roleSelective inhibitorTail fashionCrystallographic analysisMass spectrometry