2015
Mini-Osmotic Pump Infusion Model to Investigate the Systemic Effects of Chronic Continuous Exposure to Staphylococcal Superantigen in Mice
Krogman AL, Chowdhary V, Rajagopalan G. Mini-Osmotic Pump Infusion Model to Investigate the Systemic Effects of Chronic Continuous Exposure to Staphylococcal Superantigen in Mice. Methods In Molecular Biology 2015, 1396: 109-114. PMID: 26676041, PMCID: PMC4882905, DOI: 10.1007/978-1-4939-3344-0_9.Peer-Reviewed Original ResearchConceptsStaphylococcal superantigensMini-osmotic pumpsChronic continuous exposureSpectrum of diseaseSystemic effectsAcute exposureChronic exposureImmune systemRecurrent exposureSuperantigensContinuous exposureRobust activationS. aureusStaphylococcus aureusInfusion modelExposureAureusSafe wayDiseaseMiceSuperantigen-Producing Staphylococcus aureus Elicits Systemic Immune Activation in a Murine Wound Colonization Model
Kim C, Karau M, Greenwood-Quaintance K, Tilahun A, Krogman A, David C, Pritt B, Patel R, Rajagopalan G. Superantigen-Producing Staphylococcus aureus Elicits Systemic Immune Activation in a Murine Wound Colonization Model. Toxins 2015, 7: 5308-5319. PMID: 26670252, PMCID: PMC4690136, DOI: 10.3390/toxins7124886.Peer-Reviewed Original ResearchConceptsSystemic immune activationImmune activationT cellsS. aureusSerum IL-17 levelsSystemic immunologic effectsIL-17 levelsSpleens of miceWound infection modelImmunologic effectsInflammatory changesHLA-DR3Wound infectionCommon causeTransgenic miceUninfected woundsDay 8Immune systemClinical implicationsInfection modelMiceSkin woundsWoundsMicrobial challengeStaphylococcus aureus
2013
Low Incidence of Spontaneous Type 1 Diabetes in Non-Obese Diabetic Mice Raised on Gluten-Free Diets Is Associated with Changes in the Intestinal Microbiome
Marietta E, Gomez A, Yeoman C, Tilahun A, Clark C, Luckey D, Murray J, White B, Kudva Y, Rajagopalan G. Low Incidence of Spontaneous Type 1 Diabetes in Non-Obese Diabetic Mice Raised on Gluten-Free Diets Is Associated with Changes in the Intestinal Microbiome. PLOS ONE 2013, 8: e78687. PMID: 24236037, PMCID: PMC3827256, DOI: 10.1371/journal.pone.0078687.Peer-Reviewed Original ResearchConceptsGluten-free dietNon-obese diabetic (NOD) miceAnti-diabetogenic effectsIncidence of hyperglycemiaNOD miceType 1 diabetesIntestinal microbiomeDietary glutenDiabetic miceSpontaneous type 1 diabetesAkkermansia speciesIncidence of diabetesIncidence of T1DIncidence of T1D.Blood glucose levelsIntestinal microbiome compositionLower incidenceGlucose levelsHigh incidenceAnimal studiesGut microfloraGut microbiomeHyperglycemiaIncidenceMice
2012
Linezolid Is Superior to Vancomycin in Experimental Pneumonia Caused by Superantigen-Producing Staphylococcus aureus in HLA Class II Transgenic Mice
Karau M, Tilahun A, Schmidt S, Clark C, Patel R, Rajagopalan G. Linezolid Is Superior to Vancomycin in Experimental Pneumonia Caused by Superantigen-Producing Staphylococcus aureus in HLA Class II Transgenic Mice. Antimicrobial Agents And Chemotherapy 2012, 56: 5401-5405. PMID: 22850509, PMCID: PMC3457354, DOI: 10.1128/aac.01080-12.Peer-Reviewed Original ResearchConceptsHLA class II transgenic miceTransgenic miceDR3 transgenic miceII transgenic miceHuman leukocyte antigenS. aureusC57BL/6 miceLeukocyte antigenStaphylococcus aureus virulenceExperimental pneumoniaImmune systemSuperantigensPneumoniaAureus virulenceMiceBacterial protein synthesis inhibitorsProtein synthesis inhibitorStaphylococcus aureusLinezolidPotent activatorSynthesis inhibitorVancomycinAureusGreater protectionSuperior responseDetrimental effects of T regulatory cell-promoting IL-2-anti-IL-2 complexes in staphylococcal toxic shock syndrome. (164.13)
Rajagopalan G, Tilahun A. Detrimental effects of T regulatory cell-promoting IL-2-anti-IL-2 complexes in staphylococcal toxic shock syndrome. (164.13). The Journal Of Immunology 2012, 188: 164.13-164.13. DOI: 10.4049/jimmunol.188.supp.164.13.Peer-Reviewed Original ResearchToxic shock syndromeHLA-DR3 transgenic miceTreg numbersShock syndromeNon-menstrual toxic shock syndromeTransgenic miceCytokine/chemokine stormStaphylococcal toxic shock syndromeNumber of TregsMultiple organ failureStaphylococcal enterotoxin BChemokine stormMIP-1βOrgan failureMIP-1αUntreated groupSuperantigen exotoxinsDay 5Immune systemDay 0IL2CMiceEnterotoxin BStreptococcus pyogenesAdministration
2007
Delineating the Roles of CD4+ and CD8+ T Cell Subsets in the Pathogenesis of Spontaneous Autoimmune Diabetes Using HLA-DQ8 Transgenic Mice
Rajagopalan G, Mangalam A, Kudva Y, David C. Delineating the Roles of CD4+ and CD8+ T Cell Subsets in the Pathogenesis of Spontaneous Autoimmune Diabetes Using HLA-DQ8 Transgenic Mice. Clinical Immunology 2007, 123: s71. DOI: 10.1016/j.clim.2007.03.379.Peer-Reviewed Original Research
2006
Acute Systemic Immune Activation following Conjunctival Exposure to Staphylococcal Enterotoxin B
Rajagopalan G, Smart M, Patel R, David C. Acute Systemic Immune Activation following Conjunctival Exposure to Staphylococcal Enterotoxin B. Infection And Immunity 2006, 74: 6016-6019. PMID: 16988282, PMCID: PMC1594882, DOI: 10.1128/iai.00671-06.Peer-Reviewed Original Research
2004
In Vitro and In Vivo Evaluation of Staphylococcal Superantigen Peptide Antagonists
Rajagopalan G, Sen M, David C. In Vitro and In Vivo Evaluation of Staphylococcal Superantigen Peptide Antagonists. Infection And Immunity 2004, 72: 6733-6737. PMID: 15501813, PMCID: PMC522998, DOI: 10.1128/iai.72.11.6733-6737.2004.Peer-Reviewed Original Research
2003
Accelerated Diabetes in Rat Insulin Promoter-Tumor Necrosis Factor-α Transgenic Nonobese Diabetic Mice Lacking Major Histocompatibility Class II Molecules
Rajagopalan G, Kudva YC, Flavell RA, David CS. Accelerated Diabetes in Rat Insulin Promoter-Tumor Necrosis Factor-α Transgenic Nonobese Diabetic Mice Lacking Major Histocompatibility Class II Molecules. Diabetes 2003, 52: 342-347. PMID: 12540606, DOI: 10.2337/diabetes.52.2.342.Peer-Reviewed Original ResearchConceptsClass II moleculesNecrosis factorT cellsHLA class II associationsHuman type 1 diabetesMajor histocompatibility class II moleculesFunctional class II moleculesClass II associationsType 1 diabetesRat insulin promoterTumor necrosis factorLocal proinflammatory environmentIslets of LangerhansAccelerated diabetesNOD miceHLA-DQ8Proinflammatory environmentC57BL/6 miceTNF-alphaDiabetesDisease pathogenesisSignificant protectionMajor histocompatibility complex locusNeonatal expressionMice
2002
Modulation of insulitis and type 1 diabetes by transgenic HLA-DR3 and DQ8 in NOD mice lacking endogenous MHC class II
Kudva Y, Rajagopalan G, Raju R, Abraham R, Smart M, Hanson J, David C. Modulation of insulitis and type 1 diabetes by transgenic HLA-DR3 and DQ8 in NOD mice lacking endogenous MHC class II. Human Immunology 2002, 63: 987-999. PMID: 12392851, DOI: 10.1016/s0198-8859(02)00435-4.Peer-Reviewed Original ResearchConceptsType 1 diabetesEndogenous class II moleculesClass II moleculesHLA-DR3NOD miceHuman leukocyte antigen (HLA) transgenic miceTransgenic miceEndogenous MHC class IICyclophosphamide-induced diabetesIntra-islet infiltrationMultiple low dosesDouble transgenic miceGroups of miceMHC class IINonobese diabetic (NOD) backgroundTransgenic expressionSpontaneous diabetesDiabetic backgroundUnmanipulated miceDQ8DiabetesLow dosesClass IILess infiltrationMiceStaphylococcal enterotoxin B‐induced activation and concomitant resistance to cell death in CD28‐deficient HLA‐DQ8 transgenic mice
Rajagopalan G, Smart M, Marietta E, David C. Staphylococcal enterotoxin B‐induced activation and concomitant resistance to cell death in CD28‐deficient HLA‐DQ8 transgenic mice. International Immunology 2002, 14: 801-812. PMID: 12096040, DOI: 10.1093/intimm/dxf047.Peer-Reviewed Original ResearchConceptsT cellsStaphylococcal enterotoxin BHLA-DQ8IFN-gammaTransgenic miceEnterotoxin BBALB/c splenocytesDQ8 transgenic miceSerum IFN-gammaLess IL-2IL-6 productionAbsence of CD28Role of CD28Activation-induced cell deathCertain effector functionsIL-10Lethal shockIL-2IL-4Immune responseEffector functionsConcomitant resistanceCD28Nitric oxideMice