2005
Heterogeneity in synaptic transmission along a Drosophila larval motor axon
Guerrero G, Reiff D, Agarwal G, Ball R, Borst A, Goodman C, Isacoff E. Heterogeneity in synaptic transmission along a Drosophila larval motor axon. Nature Neuroscience 2005, 8: 1188-1196. PMID: 16116446, PMCID: PMC1402256, DOI: 10.1038/nn1526.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAxonsCalcium SignalingDiagnostic ImagingDrosophilaDrosophila ProteinsElectric StimulationEvoked PotentialsGene Expression Regulation, DevelopmentalImmunohistochemistryLarvaLuminescent ProteinsMembrane PotentialsMotor NeuronsMutagenesis, InsertionalNeuromuscular JunctionPatch-Clamp TechniquesSynaptic TransmissionConceptsNeuromuscular junctionGlutamate receptorsSynaptic transmissionAxonal branchesMotor axonsPostsynaptic terminalsIndividual boutonsBoutonsProximal connectionLarval neuromuscular junctionTransmission strengthLarval motor axonsDrosophila melanogaster larval neuromuscular junctionDistal boutonsDistal onesHigh Ca2Ca2InfluxAxonsReceptors
2002
Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential
Guerrero G, Siegel M, Roska B, Loots E, Isacoff E. Tuning FlaSh: Redesign of the Dynamics, Voltage Range, and Color of the Genetically Encoded Optical Sensor of Membrane Potential. Biophysical Journal 2002, 83: 3607-3618. PMID: 12496128, PMCID: PMC1302436, DOI: 10.1016/s0006-3495(02)75361-7.Peer-Reviewed Original ResearchMeSH KeywordsBiosensing TechniquesFluorescence Resonance Energy TransferGreen Fluorescent ProteinsLuminescent ProteinsMembrane PotentialsMicroscopy, FluorescenceMutagenesis, Site-DirectedNeuronsOptics and PhotonicsPotassium ChannelsPotassium Channels, Voltage-GatedProtein EngineeringRecombinant Fusion ProteinsShaker Superfamily of Potassium ChannelsConceptsDetector domainDual-wavelength measurementVoltage-gated ShakerWavelength measurementsOptical readoutOptical sensorsSpectral shiftElectrical signalsIntensity changesDistinct spectraFlashesReadoutSpectraFluorescence intensity changesVoltage dependenceDependenceVoltage rangeMeasurementsSensorsDistinct voltageVoltageDynamicsShift