2010
Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells
Marin EP, Moeckel G, Al-Lamki R, Bradley J, Yan Q, Wang T, Wright PL, Yu J, Sessa WC. Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells. American Journal Of Pathology 2010, 177: 2765-2773. PMID: 20971739, PMCID: PMC2993268, DOI: 10.2353/ajpath.2010.100199.Peer-Reviewed Original ResearchConceptsUnilateral ureteral obstructionAcute tubular necrosisEpithelial cellsRenal tubular epithelial cellsMurine kidneyIschemia/reperfusionMeasurement of fibrosisDistal nephron segmentsRecruitment of macrophagesWild-type miceInflammatory gene expressionTubular epithelial cellsDe novo expressionHuman biopsy specimensRenal injuryTubular necrosisUreteral obstructionWT miceVascular injuryHistological damageBiopsy specimensCortical tubulesDeficient miceMacrophage recruitmentTissue injuryHypertonicity-induced Mitochondrial Membrane Permeability in Renal Medullary Interstitial Cells: Protective Role of Osmolytes
Zhang L, Chen D, Chen Z, Moeckel GW. Hypertonicity-induced Mitochondrial Membrane Permeability in Renal Medullary Interstitial Cells: Protective Role of Osmolytes. Cellular Physiology And Biochemistry 2010, 25: 753-760. PMID: 20511721, PMCID: PMC3030460, DOI: 10.1159/000315095.Peer-Reviewed Original ResearchConceptsPermeability transition poreHypertonicity-induced apoptosisOrganic osmolytesCytochrome cDelta psiFluorescence probe JC-1Cell deathMitochondrial membrane permeabilityMitochondrial membrane potentialHypertonic culture conditionsMajor organic osmolytesHypertonicity-induced changesHyperosmotic stressProtein abundanceMolecular mechanismsApoptotic pathwayTransition poreJC-1OsmolytesProapoptotic BaxCytoplasmApoptosisKidney cellsMembrane potentialImmunofluorescence labeling
2009
Urea promotes TonEBP expression and cellular adaptation in extreme hypertonicity
Kwon MS, Na KY, Moeckel G, Lee SD, Kwon HM. Urea promotes TonEBP expression and cellular adaptation in extreme hypertonicity. Pflügers Archiv - European Journal Of Physiology 2009, 459: 183. PMID: 19585141, DOI: 10.1007/s00424-009-0696-5.Peer-Reviewed Original ResearchConceptsCellular adaptationRegulation of TonEBPBody water homeostasisAmbient tonicityEpithelial cell lineCentral regulatorMDCK cellsTonEBPCellular proliferationWater homeostasisCell linesExpressionPrior adaptationInner medullaAdaptationRenal medullaAntidiuretic animalsHypertonicityRegulatorHomeostasisRegulationHigh levelsProliferationCellsModerate changesPlacental Insufficiency Associated with Loss of Cited1 Causes Renal Medullary Dysplasia
Sparrow DB, Boyle SC, Sams RS, Mazuruk B, Zhang L, Moeckel GW, Dunwoodie SL, de Caestecker MP. Placental Insufficiency Associated with Loss of Cited1 Causes Renal Medullary Dysplasia. Journal Of The American Society Of Nephrology 2009, 20: 777-786. PMID: 19297558, PMCID: PMC2663829, DOI: 10.1681/asn.2008050547.Peer-Reviewed Original ResearchConceptsRenal medullary dysplasiaPlacental insufficiencyTissue oxygenationLower urinary tract obstructionUrinary tract obstructionCITED1 expressionIntrauterine growth retardationTract obstructionRenal functionMedullary growthRenal dysplasiaDysplasiaGrowth retardationRenal medullaMutant miceDecreased numberInsufficiencyEarly nephrogenesisNumber of studiesMiceGenetic lossOxygenationUreteric budCircumstantial evidenceInfants
2005
Role of integrin α1β1 in the regulation of renal medullary osmolyte concentration
Moeckel GW, Zhang L, Chen X, Rossini M, Zent R, Pozzi A. Role of integrin α1β1 in the regulation of renal medullary osmolyte concentration. American Journal Of Physiology. Renal Physiology 2005, 290: f223-f231. PMID: 16106035, DOI: 10.1152/ajprenal.00371.2004.Peer-Reviewed Original ResearchConceptsIntegrin alpha1-null miceOsmolyte accumulationTonicity enhancer-binding proteinExtracellular matrix receptors integrinsOrganic osmolytesProtective organic osmolytesMEK inhibitor PD 98059Inositol uptakeInhibitor PD 98059Enhancer-binding proteinRenal medullary cellsTubular necrosisGene transcriptionOsmolyte transportersReceptor integrinPD 98059Altered signalingDifferent osmotic conditionsOsmolyte concentrationMammalian kidneyCell behaviorImportant mediatorERK1/2 phosphorylationOsmotic conditionsDuct cells
2003
COX2 Activity Promotes Organic Osmolyte Accumulation and Adaptation of Renal Medullary Interstitial Cells to Hypertonic Stress*
Moeckel GW, Zhang L, Fogo AB, Hao CM, Pozzi A, Breyer MD. COX2 Activity Promotes Organic Osmolyte Accumulation and Adaptation of Renal Medullary Interstitial Cells to Hypertonic Stress*. Journal Of Biological Chemistry 2003, 278: 19352-19357. PMID: 12637551, DOI: 10.1074/jbc.m302209200.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAldehyde ReductaseAnimalsApoptosisBetaineCaspase 3CaspasesCell SurvivalCells, CulturedCyclooxygenase 2Cyclooxygenase 2 InhibitorsCyclooxygenase InhibitorsDehydrationDNA FragmentationGene ExpressionHeat-Shock ProteinsHypertonic SolutionsIn Situ Nick-End LabelingInositolIsoenzymesKidney MedullaMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutProstaglandin-Endoperoxide SynthasesRNA, MessengerSorbitolSymportersTritiumConceptsMedullary interstitial cellsRenal medullary interstitial cellsInterstitial cellsCOX2 activityCOX2 inhibitionReductase mRNA expressionCOX2-specific inhibitorsRenal cell survivalCell survivalAnalgesic nephropathyCOX2 inhibitorsRenal medullaMRNA expressionPmol/Sorbitol accumulationInner medullaOrganic osmolyte accumulationKidney inner medullaMedullaCell deathBetaine concentrationsHypertonic stressPresent studySurvivalInhibitors
1997
Distribution of de novo synthesized betaine in rat kidney: role of renal synthesis on medullary betaine accumulation
Moeckel G, Lien Y. Distribution of de novo synthesized betaine in rat kidney: role of renal synthesis on medullary betaine accumulation. American Journal Of Physiology 1997, 272: f94-f99. PMID: 9039054, DOI: 10.1152/ajprenal.1997.272.1.f94.Peer-Reviewed Original ResearchConceptsRenal cortexMale Sprague-Dawley ratsSprague-Dawley ratsHepatic arteryRenal arteryRenal synthesisFurosemide diuresisPortal veinOxidase inhibitorsRat kidneyKidneyTotal urineMedullary concentrationUrine samplesMedullaMedullary cellsCortexArteryDe novo synthesisBetaine concentrationsMammalian kidneyWater deprivationDe novoMinNovo synthesisKinetics and osmoregulation of Na(+)-and Cl(-)-dependent betaine transporter in rat renal medulla
Moeckel G, Lai L, Guder W, Kwon H, Lien Y. Kinetics and osmoregulation of Na(+)-and Cl(-)-dependent betaine transporter in rat renal medulla. American Journal Of Physiology 1997, 272: f100-f106. PMID: 9039055, DOI: 10.1152/ajprenal.1997.272.1.f100.Peer-Reviewed Original Research