1996
Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †
Sheptovitsky Y, Brudvig G. Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †. Biochemistry 1996, 35: 16255-16263. PMID: 8973199, DOI: 10.1021/bi9613842.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsCatalaseCatechol OxidaseChloroplastsChromatography, High Pressure LiquidChromatography, Ion ExchangeCopperElectrophoresis, Polyacrylamide GelHot TemperatureIntracellular MembranesKineticsMolecular WeightOxygenOxygen ConsumptionPeptide MappingPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexSpectrophotometrySpinacia oleraceaSubstrate SpecificityConceptsPSII membranesCentre of PSIIUV-visible absorptionO2 evolution activityThylakoid membranesSpinach thylakoid membranesAnion-exchange chromatographyMembrane-associated catalaseHeme enzymesBatch adsorptionHeme catalasesMild heat treatmentHeat treatmentMolecular weightAnion-exchange beadsNative molecular weightPPOIrreversible aggregationGel filtrationFurther manipulationAdsorptionAmino acid compositionTriazoleMembraneChromatography
1989
Molecular basis of the heat denaturation of photosystem II.
Thompson L, Blaylock R, Sturtevant J, Brudvig G. Molecular basis of the heat denaturation of photosystem II. Biochemistry 1989, 28: 6686-95. PMID: 2675973, DOI: 10.1021/bi00442a023.Peer-Reviewed Original ResearchConceptsDifferential scanning calorimetryPSII complexesMembrane proteinsMn complexesAntenna proteinsThermal gel analysisElectron paramagnetic resonance measurementsGel analysisMembrane protein complexesMajor membrane proteinParamagnetic resonance measurementsElectron transport chainRelease of MnWater oxidationA2 peakHydroxide ionPSII membranesProtein complexesAqueous phaseComponent essentialSolubility propertiesDSC studiesMolecular basisEPR experimentsPSII core