2017
Synthesis of pyridine-alkoxide ligands for formation of polynuclear complexes
Shopov D, Sharninghausen L, Sinha S, Borowski J, Mercado B, Brudvig G, Crabtree R. Synthesis of pyridine-alkoxide ligands for formation of polynuclear complexes. New Journal Of Chemistry 2017, 41: 6709-6719. DOI: 10.1039/c7nj01845b.Peer-Reviewed Original ResearchO-donor ligandsPolynuclear complexesFirst-row transition metalsPyridine-alkoxide ligandsInexpensive starting materialsOne-step synthesisX-ray crystallographyPolynuclear clustersTransition metalsStarting materialScalable procedureGeneral formulaLigandsEffective bindingLarge-scale productionLigand 2Chelation powerComplexesSynthesisDenticityCluster formationDeprotonationFormationCrystallographyChromatography
1996
Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †
Sheptovitsky Y, Brudvig G. Isolation and Characterization of Spinach Photosystem II Membrane-Associated Catalase and Polyphenol Oxidase †. Biochemistry 1996, 35: 16255-16263. PMID: 8973199, DOI: 10.1021/bi9613842.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsCatalaseCatechol OxidaseChloroplastsChromatography, High Pressure LiquidChromatography, Ion ExchangeCopperElectrophoresis, Polyacrylamide GelHot TemperatureIntracellular MembranesKineticsMolecular WeightOxygenOxygen ConsumptionPeptide MappingPhotosynthetic Reaction Center Complex ProteinsPhotosystem II Protein ComplexSpectrophotometrySpinacia oleraceaSubstrate SpecificityConceptsPSII membranesCentre of PSIIUV-visible absorptionO2 evolution activityThylakoid membranesSpinach thylakoid membranesAnion-exchange chromatographyMembrane-associated catalaseHeme enzymesBatch adsorptionHeme catalasesMild heat treatmentHeat treatmentMolecular weightAnion-exchange beadsNative molecular weightPPOIrreversible aggregationGel filtrationFurther manipulationAdsorptionAmino acid compositionTriazoleMembraneChromatography