2020
CX3CL1 homo-oligomerization drives cell-to-cell adherence
Ostuni M, Hermand P, Saindoy E, Guillou N, Guellec J, Coens A, Hattab C, Desuzinges-Mandon E, Jawhari A, Iatmanen-Harbi S, Lequin O, Fuchs P, Lacapere J, Combadière C, Pincet F, Deterre P. CX3CL1 homo-oligomerization drives cell-to-cell adherence. Scientific Reports 2020, 10: 9069. PMID: 32494000, PMCID: PMC7271195, DOI: 10.1038/s41598-020-65988-w.Peer-Reviewed Original ResearchConceptsNumerous adhesion moleculesPhotobleaching assaysNative electrophoresisAdhesive potencyTransmembrane peptidesLipid environmentKey immune processesAdhesive functionFluorescence recoveryFunctional roleDomain peptideFluorescence kineticsOligomerizationCellular adherenceMolecular modelingAdhesion moleculesCell adherenceTransmembrane chemokineImmune processesCompact bundlePeptidesBlood leukocytesClustersElectrophoresisCX3CL1
2017
Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering
Šmít D, Fouquet C, Pincet F, Zapotocky M, Trembleau A. Axon tension regulates fasciculation/defasciculation through the control of axon shaft zippering. ELife 2017, 6: e19907. PMID: 28422009, PMCID: PMC5478281, DOI: 10.7554/elife.19907.Peer-Reviewed Original Research
2014
Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization
Chalbi M, Barraud-Lange V, Ravaux B, Howan K, Rodriguez N, Soule P, Ndzoudi A, Boucheix C, Rubinstein E, Wolf J, Ziyyat A, Perez E, Pincet F, Gourier C. Binding of sperm protein Izumo1 and its egg receptor Juno drives Cd9 accumulation in the intercellular contact area prior to fusion during mammalian fertilization. Development 2014, 141: 3732-3739. PMID: 25209248, DOI: 10.1242/dev.111534.Peer-Reviewed Original ResearchConceptsGamete fusionMammalian fertilizationMolecular mechanismsSperm protein IZUMO1Intercellular contact areaFusion machineryMembrane proteinsMembrane organizationIZUMO1Intercellular adhesionAdhesion partnersRecruitment kineticsKey playersCD9Adhesion phaseEggsAdhesion areaFertilizationFusionHuman eggsGametesMachineryAdhesionSpeciesProtein
2013
Homotypic and Heterotypic Adhesion Induced by Odorant Receptors and the β2-Adrenergic Receptor
Richard M, Jamet S, Fouquet C, Dubacq C, Boggetto N, Pincet F, Gourier C, Trembleau A. Homotypic and Heterotypic Adhesion Induced by Odorant Receptors and the β2-Adrenergic Receptor. PLOS ONE 2013, 8: e80100. PMID: 24312457, PMCID: PMC3846556, DOI: 10.1371/journal.pone.0080100.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAxonsCell AdhesionCell Line, TumorMiceReceptors, Adrenergic, beta-2Receptors, OdorantSensory Receptor CellsConceptsG protein-coupled receptorsΒ2-adrenergic receptorOlfactory axonsAxon sortingOlfactory sensory neuron projectionsSensory neuron projectionsSubpopulation of axonsMouse olfactory systemOdorant receptorsProtein-coupled receptorsHeterotypic adhesionSensory neuronsAdrenergic receptorsAxonal wiringNeuron projectionsAxonsReceptorsOlfactory systemMOR256-17First evidenceCritical determinant
2011
CD9 tetraspanin generates fusion competent sites on the egg membrane for mammalian fertilization
Jégou A, Ziyyat A, Barraud-Lange V, Perez E, Wolf J, Pincet F, Gourier C. CD9 tetraspanin generates fusion competent sites on the egg membrane for mammalian fertilization. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 10946-10951. PMID: 21690351, PMCID: PMC3131345, DOI: 10.1073/pnas.1017400108.Peer-Reviewed Original ResearchConceptsAdhesion sitesFusion-competent sitesWild-type eggsCD9 tetraspaninEgg membrane proteinsSperm-egg fusionMembrane proteinsGamete membranesMammalian fertilizationCompetent sitesGamete interactionAdhesion eventsTight proximityEgg membraneCD9TetraspaninsEggsFertilizationMembraneStrong-interaction modesAdhesion probabilitySitesFusionInteraction modesProtein
2010
Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractility
Martinez-Rico C, Pincet F, Thiery JP, Dufour S. Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractility. Journal Of Cell Science 2010, 123: 712-722. PMID: 20144995, DOI: 10.1242/jcs.047878.Peer-Reviewed Original ResearchConceptsIntercellular adhesionActomyosin contractilityCell doubletsE-cadherin-mediated intercellular adhesionIntercellular adhesion strengthSrc family kinasesCell-matrix interactionsSrc kinase activationExistence of crosstalkRole of integrinsFamily kinasesCadherin-7Cell plasticityMolecular crosstalkMajor adhesion moleculeIntegrin stimulationCell spreadingCadherinCell adhesionII activityIntegrinsPolylysine-coated beadsAdhesion moleculesCrosstalkAdhesionThe adhesion mediated by the P-selectin P–selectin glycoprotein ligand-1 (PSGL-1) couple is stronger for shorter PSGL-1 variants
Barbaux S, Poirier O, Pincet F, Hermand P, Tiret L, Deterre P. The adhesion mediated by the P-selectin P–selectin glycoprotein ligand-1 (PSGL-1) couple is stronger for shorter PSGL-1 variants. Journal Of Leukocyte Biology 2010, 87: 727-734. PMID: 20051472, DOI: 10.1189/jlb.0609408.Peer-Reviewed Original Research
2008
Functional Adhesiveness of the CX3CL1 Chemokine Requires Its Aggregation ROLE OF THE TRANSMEMBRANE DOMAIN*
Hermand P, Pincet F, Carvalho S, Ansanay H, Trinquet E, Daoudi M, Combadière C, Deterre P. Functional Adhesiveness of the CX3CL1 Chemokine Requires Its Aggregation ROLE OF THE TRANSMEMBRANE DOMAIN*. Journal Of Biological Chemistry 2008, 283: 30225-30234. PMID: 18725411, PMCID: PMC2662081, DOI: 10.1074/jbc.m802638200.Peer-Reviewed Original ResearchConceptsBioluminescence resonance energy transferHomogeneous time-resolved fluorescenceTransmembrane domainAdhesive potencyTransmembrane domain residuesLoss of glycosylationConstitutive oligomersDomain residuesBRET signalTruncation experimentsResonance energy transferCell surfacePrimary cellsSpecific signalsNative formAdhesion assaysAdhesive moleculesCell linesCentral roleAggregation roleInhibition of CX3CL1New pathwayTime-resolved fluorescenceCellsAssays
2007
Mapping Mouse Gamete Interaction Forces Reveal Several Oocyte Membrane Regions with Different Mechanical and Adhesive Properties †
Jégou A, Pincet F, Perez E, Wolf JP, Ziyyat A, Gourier C. Mapping Mouse Gamete Interaction Forces Reveal Several Oocyte Membrane Regions with Different Mechanical and Adhesive Properties †. Langmuir 2007, 24: 1451-1458. PMID: 18027975, DOI: 10.1021/la702258x.Peer-Reviewed Original Research
2005
Prototypical Type I E-cadherin and Type II Cadherin-7 Mediate Very Distinct Adhesiveness through Their Extracellular Domains*
Chu YS, Eder O, Thomas WA, Simcha I, Pincet F, Ben-Ze'ev A, Perez E, Thiery JP, Dufour S. Prototypical Type I E-cadherin and Type II Cadherin-7 Mediate Very Distinct Adhesiveness through Their Extracellular Domains*. Journal Of Biological Chemistry 2005, 281: 2901-2910. PMID: 16253998, DOI: 10.1074/jbc.m506185200.Peer-Reviewed Original ResearchConceptsType II cadherinsE-cadherinIntercellular adhesivenessChimeric cadherinsTissue morphogenesisCadherin expression levelsCytoskeletal complexCytoplasmic tailCadherin-7Adherent junctionsCell doubletsCadherinExtracellular domainType ICell adhesivityExpression levelsStable complexesLow adhesivityCellsMorphogenesisAdhesivityComplexesProfound implicationsHigh adhesivityRegulationJohnson-Kendall-Roberts Theory Applied to Living Cells
Chu YS, Dufour S, Thiery JP, Perez E, Pincet F. Johnson-Kendall-Roberts Theory Applied to Living Cells. Physical Review Letters 2005, 94: 028102. PMID: 15698233, DOI: 10.1103/physrevlett.94.028102.Peer-Reviewed Original Research
2004
Force measurements in E-cadherin–mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42
Chu Y, Thomas W, Eder O, Pincet F, Perez E, Thiery J, Dufour S. Force measurements in E-cadherin–mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42. Journal Of Cell Biology 2004, 167: 1183-1194. PMID: 15596540, PMCID: PMC2172605, DOI: 10.1083/jcb.200403043.Peer-Reviewed Original ResearchConceptsActin cytoskeletonCadherin-dependent cell-cell adhesionE-cadherin-based adhesionsE-cadherin-expressing cellsCell adhesionActin cytoskeleton dynamicsRho-like small GTPasesCell-cell adhesionDominant active formDominant-negative formStrong cell adhesionFunctional cadherinCytoskeleton dynamicsSmall GTPasesCell doubletsHomophilic interactionsActin polymerizationIntercellular adhesionCdc42Negative formCadherinCell surfaceCadherin levelsCytoskeletonRacSeparation Force Measurements Reveal Different Types of Modulation of E-cadherin-based Adhesion by Nectin-1 and -3*
Martinez-Rico C, Pincet F, Perez E, Thiery J, Shimizu K, Takai Y, Dufour S. Separation Force Measurements Reveal Different Types of Modulation of E-cadherin-based Adhesion by Nectin-1 and -3*. Journal Of Biological Chemistry 2004, 280: 4753-4760. PMID: 15550395, DOI: 10.1074/jbc.m412544200.Peer-Reviewed Original ResearchConceptsE-cadherin-based adhesionsNectin-3E-cadherin-dependent cell adhesionExtracellular fragmentE-cadherin-mediated adhesionE-cadherin-expressing cellsNectin-1Cell adhesionCell-cell adhesionIndependent cell adhesion moleculesAdherens junctionsCell adhesion moleculeCell doubletsSignificant agonistic effectKey moleculesE-cadherinL cellsCadherinNectinAdhesion moleculesCellsAdhesionFragmentsHomodimerHeterodimersSpecific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics
Sarda S, Pointu D, Pincet F, Henry N. Specific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics. Biophysical Journal 2004, 86: 3291-3303. PMID: 15111442, PMCID: PMC1304194, DOI: 10.1016/s0006-3495(04)74377-5.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD19Binding SitesBiophysicsBiotinylationCell AdhesionCell Line, TransformedCell Line, TumorCell MembraneDiffusionFlow CytometryHumansKineticsLigandsLymphoma, B-CellMicroscopy, FluorescenceModels, BiologicalProtein BindingScattering, RadiationStreptavidinStress, MechanicalSurface PropertiesTemperatureTime FactorsEnhanced Adhesive Capacities of the Naturally Occurring Ile249–Met280 Variant of the Chemokine Receptor CX3CR1*
Daoudi M, Lavergne E, Garin A, Tarantino N, Debré P, Pincet F, Combadière C, Deterre P. Enhanced Adhesive Capacities of the Naturally Occurring Ile249–Met280 Variant of the Chemokine Receptor CX3CR1*. Journal Of Biological Chemistry 2004, 279: 19649-19657. PMID: 14990582, DOI: 10.1074/jbc.m313457200.Peer-Reviewed Original Research
2001
Ultraweak Sugar-Sugar Interactions for Transient Cell Adhesion
Pincet F, Le Bouar T, Zhang Y, Esnault J, Mallet J, Perez E, Sinaÿ P. Ultraweak Sugar-Sugar Interactions for Transient Cell Adhesion. Biophysical Journal 2001, 80: 1354-1358. PMID: 11222296, PMCID: PMC1301327, DOI: 10.1016/s0006-3495(01)76108-5.Peer-Reviewed Original Research