2018
Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+
Gruget C, Coleman J, Bello O, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH. Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+. FEBS Letters 2018, 592: 1497-1506. PMID: 29578584, DOI: 10.1002/1873-3468.13040.Peer-Reviewed Original Research
2014
A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane Fusion
Li F, Kümmel D, Coleman J, Reinisch KM, Rothman JE, Pincet F. A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane Fusion. Journal Of The American Chemical Society 2014, 136: 3456-3464. PMID: 24533674, PMCID: PMC3985920, DOI: 10.1021/ja410690m.Peer-Reviewed Original ResearchConceptsN-terminal domainMembrane fusionV-SNARET-SNAREsRecent biophysical studiesC-terminal portionSNARE complexTransmembrane domainRegulatory proteinsFunctional intermediatesC-terminusDistinct functionsN-terminusMolecular mechanismsConformational rearrangementsBiophysical studiesVital regulatorZippering mechanismRate-limiting stepBiological membranesSnareFusionComplexinMultiple stagesZippering
2009
Force spectroscopy of a single artificial biomolecule bond: The Kramers’ high-barrier limit holds close to the critical force
Husson J, Dogterom M, Pincet F. Force spectroscopy of a single artificial biomolecule bond: The Kramers’ high-barrier limit holds close to the critical force. The Journal Of Chemical Physics 2009, 130: 051103. PMID: 19206951, DOI: 10.1063/1.3077010.Peer-Reviewed Original ResearchConceptsHigh-force regimeHigh barrier limitOptical tweezersEnergy barrierMicron-size beadsKramers approximationBarrier energyForce spectroscopyHigh energy barrierExponential decayEnergy profilesThermal energyEnergyApproximationTweezersBond studyCritical forceRecent modelsDecayKinetics of escapeSpectroscopyKramerRegimeBondsForce
2007
Confinement Free Energy of Surfaces Bearing End-Grafted Polymers in the Mushroom Regime and Local Measurement of the Polymer Density
Li F, Pincet F. Confinement Free Energy of Surfaces Bearing End-Grafted Polymers in the Mushroom Regime and Local Measurement of the Polymer Density. Langmuir 2007, 23: 12541-12548. PMID: 17988162, DOI: 10.1021/la7021374.Peer-Reviewed Original ResearchConceptsPolymer chainsMushroom regimeFree energyEnd-Grafted PolymersSurface force measurementsSingle polymer chainSurface force apparatusEnd-tethered polymer chainsInteraction free energyGrafted polymersForce apparatusPolymer densityForce profilesPolymersConfinement free energyPolymer theoryLipid bilayersIdentical surfacesMushroomlike structuresFree energy approximationForce measurementsSurfacePlanar surfaceGood precisionChain
2005
Spontaneous and Reversible Switch from Amphiphilic to Oil-Like Structures
Pincet F, Tareste D, Amar M, Perez É. Spontaneous and Reversible Switch from Amphiphilic to Oil-Like Structures. Physical Review Letters 2005, 95: 218101. PMID: 16384186, DOI: 10.1103/physrevlett.95.218101.Peer-Reviewed Original Research
2001
Ultraweak Sugar-Sugar Interactions for Transient Cell Adhesion
Pincet F, Le Bouar T, Zhang Y, Esnault J, Mallet J, Perez E, Sinaÿ P. Ultraweak Sugar-Sugar Interactions for Transient Cell Adhesion. Biophysical Journal 2001, 80: 1354-1358. PMID: 11222296, PMCID: PMC1301327, DOI: 10.1016/s0006-3495(01)76108-5.Peer-Reviewed Original Research