2020
Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein
Heo P, Pincet F. Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein. Communications Biology 2020, 3: 148. PMID: 32235856, PMCID: PMC7109109, DOI: 10.1038/s42003-020-0883-7.Peer-Reviewed Original ResearchMeSH KeywordsAlpha-SynucleinCell MembraneElectric CapacitanceFluorescence Recovery After PhotobleachingHydrophobic and Hydrophilic InteractionsLab-On-A-Chip DevicesMembrane FluidityMembrane LipidsMembrane PotentialsMembranes, ArtificialMicrofluidic Analytical TechniquesNeuronsProtein AggregatesProtein Aggregation, PathologicalProtein BindingProtein ConformationStructure-Activity RelationshipSynucleinopathiesConceptsPlasma membraneMembrane-bound proteinsAccumulation of aggregatesΑ-synucleinCytosolic leafletMembrane topologyMembrane hydrophobic coreCytosolic proteinsProteinExtracellular onesHydrophobic corePathological roleDiscrete sizesMembraneLeafletsMembrane capacitanceNeurological diseasesLipidsAccumulationMicrofluidic setup
2019
SNARE machinery is optimized for ultrafast fusion
Manca F, Pincet F, Truskinovsky L, Rothman JE, Foret L, Caruel M. SNARE machinery is optimized for ultrafast fusion. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 2435-2442. PMID: 30700546, PMCID: PMC6377469, DOI: 10.1073/pnas.1820394116.Peer-Reviewed Original Research
2018
Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+
Gruget C, Coleman J, Bello O, Krishnakumar SS, Perez E, Rothman JE, Pincet F, Donaldson SH. Rearrangements under confinement lead to increased binding energy of Synaptotagmin‐1 with anionic membranes in Mg2+ and Ca2+. FEBS Letters 2018, 592: 1497-1506. PMID: 29578584, DOI: 10.1002/1873-3468.13040.Peer-Reviewed Original ResearchMeSH KeywordsCalciumCell MembraneDose-Response Relationship, DrugMagnesiumProtein BindingSurface PropertiesSynaptotagmin IThermodynamics
2016
A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion
Xu W, Nathwani B, Lin C, Wang J, Karatekin E, Pincet F, Shih W, Rothman JE. A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion. Journal Of The American Chemical Society 2016, 138: 4439-4447. PMID: 26938705, PMCID: PMC4950518, DOI: 10.1021/jacs.5b13107.Peer-Reviewed Original ResearchMeSH KeywordsDNADNA, Single-StrandedLipid BilayersLiposomesMembrane FusionProtein BindingSNARE ProteinsVesicular Transport ProteinsConceptsMembrane fusionSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexCore molecular machineryMembrane fusion eventsProtein receptor complexMembrane fusion processMolecular machineryDNA origami platformTarget membraneAuxiliary proteinsIntracellular communicationDocking stepSingle-event levelReceptor complexLipid mixingSmall unilamellar vesiclesLipid bilayersSnareFundamental processesVesiclesUnilamellar vesiclesTraffickingMachineryProteinFusion
2010
The adhesion mediated by the P-selectin P–selectin glycoprotein ligand-1 (PSGL-1) couple is stronger for shorter PSGL-1 variants
Barbaux S, Poirier O, Pincet F, Hermand P, Tiret L, Deterre P. The adhesion mediated by the P-selectin P–selectin glycoprotein ligand-1 (PSGL-1) couple is stronger for shorter PSGL-1 variants. Journal Of Leukocyte Biology 2010, 87: 727-734. PMID: 20051472, DOI: 10.1189/jlb.0609408.Peer-Reviewed Original Research
2005
The Solution to the Streptavidin-Biotin Paradox: The Influence of History on the Strength of Single Molecular Bonds
Pincet F, Husson J. The Solution to the Streptavidin-Biotin Paradox: The Influence of History on the Strength of Single Molecular Bonds. Biophysical Journal 2005, 89: 4374-4381. PMID: 16169976, PMCID: PMC1367001, DOI: 10.1529/biophysj.105.067769.Peer-Reviewed Original ResearchGiant vesicles formed by gentle hydration and electroformation: A comparison by fluorescence microscopy
Rodriguez N, Pincet F, Cribier S. Giant vesicles formed by gentle hydration and electroformation: A comparison by fluorescence microscopy. Colloids And Surfaces B Biointerfaces 2005, 42: 125-130. PMID: 15833663, DOI: 10.1016/j.colsurfb.2005.01.010.Peer-Reviewed Original Research
2004
Separation Force Measurements Reveal Different Types of Modulation of E-cadherin-based Adhesion by Nectin-1 and -3*
Martinez-Rico C, Pincet F, Perez E, Thiery J, Shimizu K, Takai Y, Dufour S. Separation Force Measurements Reveal Different Types of Modulation of E-cadherin-based Adhesion by Nectin-1 and -3*. Journal Of Biological Chemistry 2004, 280: 4753-4760. PMID: 15550395, DOI: 10.1074/jbc.m412544200.Peer-Reviewed Original ResearchConceptsE-cadherin-based adhesionsNectin-3E-cadherin-dependent cell adhesionExtracellular fragmentE-cadherin-mediated adhesionE-cadherin-expressing cellsNectin-1Cell adhesionCell-cell adhesionIndependent cell adhesion moleculesAdherens junctionsCell adhesion moleculeCell doubletsSignificant agonistic effectKey moleculesE-cadherinL cellsCadherinNectinAdhesion moleculesCellsAdhesionFragmentsHomodimerHeterodimersSpecific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics
Sarda S, Pointu D, Pincet F, Henry N. Specific Recognition of Macroscopic Objects by the Cell Surface: Evidence for a Receptor Density Threshold Revealed by Micrometric Particle Binding Characteristics. Biophysical Journal 2004, 86: 3291-3303. PMID: 15111442, PMCID: PMC1304194, DOI: 10.1016/s0006-3495(04)74377-5.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD19Binding SitesBiophysicsBiotinylationCell AdhesionCell Line, TransformedCell Line, TumorCell MembraneDiffusionFlow CytometryHumansKineticsLigandsLymphoma, B-CellMicroscopy, FluorescenceModels, BiologicalProtein BindingScattering, RadiationStreptavidinStress, MechanicalSurface PropertiesTemperatureTime FactorsEnhanced Adhesive Capacities of the Naturally Occurring Ile249–Met280 Variant of the Chemokine Receptor CX3CR1*
Daoudi M, Lavergne E, Garin A, Tarantino N, Debré P, Pincet F, Combadière C, Deterre P. Enhanced Adhesive Capacities of the Naturally Occurring Ile249–Met280 Variant of the Chemokine Receptor CX3CR1*. Journal Of Biological Chemistry 2004, 279: 19649-19657. PMID: 14990582, DOI: 10.1074/jbc.m313457200.Peer-Reviewed Original Research