2020
In vitro physiological membrane‐on‐a‐chip and its application in cell and neuronal biology
Heo P, Rothman J, Pincet F. In vitro physiological membrane‐on‐a‐chip and its application in cell and neuronal biology. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.08637.Peer-Reviewed Original ResearchNeuronal biologyTail-anchored proteinsPost-translational insertionMost biological processesAreas of biologyVesicle traffickingProtein insertionER membranePhysiological lipid compositionBiological processesMolecular mechanismsBiologyLipid compositionPhysiological membranesModel membranesMembraneInvaluable insightsCellsTraffickingProteinInsertionPatch-clamp amplifierΑ-synucleinopathiesCompositionBilayers
2019
Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic Setup
Heo P, Ramakrishnan S, Coleman J, Rothman JE, Fleury J, Pincet F. Highly Reproducible Physiological Asymmetric Membrane with Freely Diffusing Embedded Proteins in a 3D‐Printed Microfluidic Setup. Small 2019, 15: e1900725. PMID: 30977975, DOI: 10.1002/smll.201900725.Peer-Reviewed Original ResearchConceptsMost biological processesLipid leafletAreas of biologyEmbedded proteinsBiological processesRelevant lipidsProteinAsymmetric bilayersPhysiological conditionsModel membranesPlanar bilayersBilayer formation processInvaluable insightsBilayersConfocal microscopeMembraneLipidsTransmembraneBiologyLeafletsMicrofluidic setupRecapitulation
2001
Short-range specific forces are able to induce hemifusion
Pincet F, Lebeau L, Cribier S. Short-range specific forces are able to induce hemifusion. European Biophysics Journal 2001, 30: 91-97. PMID: 11409468, DOI: 10.1007/s002490100131.Peer-Reviewed Original ResearchConceptsDepletion forcesInterbilayer distanceAdditional attractive forceAmphiphilic moleculesNucleic basesPolar headBiological membranesLipidic systemsModel membranesPossible structuresFluorescent lipid analogsAttractive forceLow water contentLamellar structureLipid analoguesExtra attractionLipid rearrangementBase pairingWater contentMembrane behaviorNucleosidesPhase transitionMembraneMoleculesAdherent vesicles