2009
siRNA-based targeting of antiapoptotic genes can reverse chemoresistance in P-glycoprotein expressing chondrosarcoma cells
Kim DW, Kim KO, Shin MJ, Ha JH, Seo SW, Yang J, Lee FY. siRNA-based targeting of antiapoptotic genes can reverse chemoresistance in P-glycoprotein expressing chondrosarcoma cells. Molecular Cancer 2009, 8: 28. PMID: 19445670, PMCID: PMC2689171, DOI: 10.1186/1476-4598-8-28.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic AgentsApoptosisATP Binding Cassette Transporter, Subfamily B, Member 1Bcl-X ProteinBone NeoplasmsCell Line, TumorChondrosarcomaDoxorubicinDrug Resistance, NeoplasmFlow CytometryGene SilencingGenes, bcl-2HumansImmunoblottingReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingX-Linked Inhibitor of Apoptosis ProteinConceptsAntiapoptotic proteinsChondrosarcoma cellsAntiapoptotic genesPharmacologic inhibitorsCell survival proteinsChondrosarcoma cell linesSurvival proteinsP-glycoproteinSiRNA knockdownCell typesProteinMechanisms of chemoresistanceCell linesGenesCommon mechanismChemoresistanceSiRNACellsP-glycoprotein inhibitionTherapeutic strategiesInhibitorsKnockdownApoptosisJJ012ConclusionThese findings
2005
μ-Calpain Regulates Receptor Activator of NF-κB Ligand (RANKL)-supported Osteoclastogenesis via NF-κB Activation in RAW 264.7 Cells*
Lee FY, Kim DW, Karmin JA, Hong D, Chang SS, Fujisawa M, Takayanagi H, Bigliani LU, Blaine TA, Lee HJ. μ-Calpain Regulates Receptor Activator of NF-κB Ligand (RANKL)-supported Osteoclastogenesis via NF-κB Activation in RAW 264.7 Cells*. Journal Of Biological Chemistry 2005, 280: 29929-29936. PMID: 15955824, DOI: 10.1074/jbc.m414600200.Peer-Reviewed Original ResearchConceptsRAW 264.7 cellsNF-kappaB activationReceptor activatorCalpain inhibitorsCell-permeable calpain inhibitorMatrix metalloproteinase-9Regulation of RANKLNF-κB activationNF-κB ligandNF-kappaB ligandMonocyte/macrophage progenitorsRole of calpainMurine RAW 264.7 cellsCell typesMetalloproteinase-9Osteoclastogenic markersCalpain activationResistant acidDecreased expressionOsteoclastogenesisRANKLCalpain activityMu-calpainInhibitorsActivation