Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function
Eva C, Bovolin P, Balzac F, Botta C, Ricci Gamalero S, Vaccarino F. Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function. Journal Of Molecular Neuroscience 1990, 2: 143. PMID: 2177349, DOI: 10.1007/bf02896839.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenylyl CyclasesAnimalsAnimals, NewbornCarbacholCells, CulturedCerebral CortexCholine O-AcetyltransferaseCorpus StriatumKineticsNeuronsPhosphatidylinositolsPolymerase Chain ReactionQuinuclidinyl BenzilateRadioligand AssayRatsReceptors, MuscarinicRNA, MessengerSecond Messenger SystemsConceptsAmount of acetylcholineDensity of mAChRsAdult rat cortexCholinergic receptorsRat cortexNeuronal culturesCholine acetyltransferase-immunoreactive neuronsPrimary culturesMuscarinic cholinergic receptorsTranscriptase-polymerase chain reaction techniqueReverse transcriptase-polymerase chain reaction techniqueNewborn rat brainAdenylate cyclase activityCorticostriatal cellsCorticostriatal neuronsImmunoreactive neuronsSubtype mRNAsChain reaction techniqueMuscarinic antagonistCholinergic transmissionMuscarinic agonistsSecond messenger systemsPostsynaptic componentsNewborn ratsRat brain