2017
One thousand somatic SNVs per skin fibroblast cell set baseline of mosaic mutational load with patterns that suggest proliferative origin
Abyzov A, Tomasini L, Zhou B, Vasmatzis N, Coppola G, Amenduni M, Pattni R, Wilson M, Gerstein M, Weissman S, Urban AE, Vaccarino FM. One thousand somatic SNVs per skin fibroblast cell set baseline of mosaic mutational load with patterns that suggest proliferative origin. Genome Research 2017, 27: 512-523. PMID: 28235832, PMCID: PMC5378170, DOI: 10.1101/gr.215517.116.Peer-Reviewed Original ResearchConceptsSomatic mosaicismFibroblast cellsSingle-cell whole-genome amplificationAllele frequenciesNumber of SNVsNormal cell proliferationCell proliferationWhole genome amplificationStem cell linesPluripotent stem cell lineHealthy human tissuesDe novo variantsCancer mutationsHigh-resolution analysisMutational loadPCR experimentsSkin fibroblast cellsMutational signaturesHiPSC linesDe novoGenomeNovo variantsFibroblast populationsCell linesSomatic SNVs
2015
Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1+/− patients and in foxg1+/− mice
Patriarchi T, Amabile S, Frullanti E, Landucci E, Lo Rizzo C, Ariani F, Costa M, Olimpico F, W Hell J, M Vaccarino F, Renieri A, Meloni I. Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1+/− patients and in foxg1+/− mice. European Journal Of Human Genetics 2015, 24: 871-880. PMID: 26443267, PMCID: PMC4820038, DOI: 10.1038/ejhg.2015.216.Peer-Reviewed Original ResearchConceptsRett syndromeSynaptic markersInhibitory synapsesExcitatory/inhibitory balanceSynaptic protein expressionFetal mouse brainInhibitory synaptic markersPathogenesis of RTTExcitatory synaptic markersSevere neurodevelopmental disorderGlutamatergic markersInhibitory balanceAdult brainAdult micePrecise molecular mechanismsSynaptic differentiationPatientsMouse brainBrain synapsesPathological eventsNeuronsProtein expressionBrainGluD1Neurodevelopmental disorders
2012
Somatic copy number mosaicism in human skin revealed by induced pluripotent stem cells
Abyzov A, Mariani J, Palejev D, Zhang Y, Haney MS, Tomasini L, Ferrandino AF, Rosenberg Belmaker LA, Szekely A, Wilson M, Kocabas A, Calixto NE, Grigorenko EL, Huttner A, Chawarska K, Weissman S, Urban AE, Gerstein M, Vaccarino FM. Somatic copy number mosaicism in human skin revealed by induced pluripotent stem cells. Nature 2012, 492: 438-442. PMID: 23160490, PMCID: PMC3532053, DOI: 10.1038/nature11629.Peer-Reviewed Original ResearchOligodendrocyte Regeneration after Neonatal Hypoxia Requires FoxO1-Mediated p27Kip1 Expression
Jablonska B, Scafidi J, Aguirre A, Vaccarino F, Nguyen V, Borok E, Horvath TL, Rowitch DH, Gallo V. Oligodendrocyte Regeneration after Neonatal Hypoxia Requires FoxO1-Mediated p27Kip1 Expression. Journal Of Neuroscience 2012, 32: 14775-14793. PMID: 23077062, PMCID: PMC3517297, DOI: 10.1523/jneurosci.2060-12.2012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCell DifferentiationCells, CulturedCyclin-Dependent Kinase Inhibitor p27Forkhead Box Protein O1Forkhead Transcription FactorsGene Expression Regulation, DevelopmentalHumansHypoxia, BrainInfantInfant, NewbornMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMice, TransgenicNerve RegenerationOligodendrogliaConceptsDiffuse white matter injuryNeonatal hypoxiaOligodendrocyte regenerationOligodendrocyte progenitor cell proliferationWhite matter injuryWhite matter lesionsPermanent neurodevelopmental disabilityCritical developmental time windowWhite matter developmentOverexpression of FoxO1Preterm infantsProgenitor cell proliferationDevelopmental time windowMatter lesionsOligodendrocyte deathAbnormal myelinationNeurodevelopmental disabilitiesMouse modelBiphasic effectP27Kip1 expressionNull miceOligodendrogenesisHypoxiaOligodendrocyte differentiationOligodendrocyte developmentModeling human cortical development in vitro using induced pluripotent stem cells
Mariani J, Simonini MV, Palejev D, Tomasini L, Coppola G, Szekely AM, Horvath TL, Vaccarino FM. Modeling human cortical development in vitro using induced pluripotent stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 12770-12775. PMID: 22761314, PMCID: PMC3411972, DOI: 10.1073/pnas.1202944109.Peer-Reviewed Original ResearchConceptsHuman brain developmentHuman induced pluripotent stem cellsLayer-specific cortical neuronsBrain developmentHuman cerebral cortexHuman cortical developmentStem cellsPluripotent stem cellsCerebral cortexCortical neuronsCortical developmentCNS regionsRadial gliaCortical wallDorsal telencephalonEmbryonic telencephalonGene expression profilesInduced pluripotent stem cellsIntermediate progenitorsTelencephalic developmentTelencephalonExpression profilesTranscriptional programsCellsGlia
2011
FGF Signaling Expands Embryonic Cortical Surface Area by Regulating Notch-Dependent Neurogenesis
Rash BG, Lim HD, Breunig JJ, Vaccarino FM. FGF Signaling Expands Embryonic Cortical Surface Area by Regulating Notch-Dependent Neurogenesis. Journal Of Neuroscience 2011, 31: 15604-15617. PMID: 22031906, PMCID: PMC3235689, DOI: 10.1523/jneurosci.4439-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnalysis of VarianceAnimalsBrainBromodeoxyuridineCaspase 3Cell CountCell DifferentiationCells, CulturedCerebral CortexDNA-Binding ProteinsElectroporationEmbryo, MammalianEye ProteinsFatty Acid-Binding Protein 7Fatty Acid-Binding ProteinsFibroblast Growth FactorsGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsHomeodomain ProteinsKi-67 AntigenMiceMice, TransgenicMutationNerve Tissue ProteinsNeurogenesisNeuronsPaired Box Transcription FactorsPAX6 Transcription FactorReceptors, Fibroblast Growth FactorReceptors, NotchRepressor ProteinsSignal TransductionStem CellsT-Box Domain ProteinsTranscription FactorsConceptsCortical neurogenesisCortical surface area expansionCortical surface expansionCortical surface areaGrowth factor receptorEmbryonic day 12.5Fibroblast growth factor receptorFGFR mutantsNormal miceCortical layer structureCortical developmentNeurogenic stagesDominant negative FGFRLoss of functionRadial progenitorsNeurogenesisNotch pathway genesSevere deficitsFactor receptorDay 12.5Notch pathwayMiceSimultaneous activationGreater proportionFGFR activityCortical Glial Fibrillary Acidic Protein-Positive Cells Generate Neurons after Perinatal Hypoxic Injury
Bi B, Salmaso N, Komitova M, Simonini MV, Silbereis J, Cheng E, Kim J, Luft S, Ment LR, Horvath TL, Schwartz ML, Vaccarino FM. Cortical Glial Fibrillary Acidic Protein-Positive Cells Generate Neurons after Perinatal Hypoxic Injury. Journal Of Neuroscience 2011, 31: 9205-9221. PMID: 21697371, PMCID: PMC3142780, DOI: 10.1523/jneurosci.0518-11.2011.Peer-Reviewed Original ResearchConceptsGlial fibrillary acidic protein-positive cellsCortical excitatory neuronsProtein-positive cellsPerinatal hypoxic injuryPostnatal hypoxiaGenetic fate mappingCortical astrogliaPremature childrenHypoxic injuryBrain injuryNew neuronsPreterm childrenNeurogenic nicheCognitive recoveryExcitatory neuronsGenerate neuronsNeuronal fateNeuronsHypoxiaCortical parenchymaInjuryParenchymaFate mappingCellsChildren
2004
Fibroblast Growth Factor Receptor 1 Is Required for the Proliferation of Hippocampal Progenitor Cells and for Hippocampal Growth in Mouse
Ohkubo Y, Uchida AO, Shin D, Partanen J, Vaccarino FM. Fibroblast Growth Factor Receptor 1 Is Required for the Proliferation of Hippocampal Progenitor Cells and for Hippocampal Growth in Mouse. Journal Of Neuroscience 2004, 24: 6057-6069. PMID: 15240797, PMCID: PMC6729672, DOI: 10.1523/jneurosci.1140-04.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, DifferentiationCell CountCell LineageCell ProliferationCells, CulturedHeredodegenerative Disorders, Nervous SystemHippocampusHumansIn Situ HybridizationLateral VentriclesMiceMice, TransgenicMutagenesis, Site-DirectedNeurogliaNeuronsPyramidal CellsReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptors, Fibroblast Growth FactorRNA, MessengerStem CellsTransgenesConceptsHippocampal ventricular zonesDentate gyrusGrowth factor receptor 1Fibroblast growth factor receptor 1Factor receptor 1Ventricular zoneNeural stem cellsPyramidal neuronsHippocampal growthProgenitor cellsGranule cellsReceptor 1Glial fibrillary acidic protein promoterHuman glial fibrillary acidic protein promoterEmbryonic dorsal telencephalonRadial glial-like cellsRadial glial progenitor cellsHippocampal dentate gyrusParvalbumin-containing interneuronsDG granule cellsHippocampal pyramidal neuronsStem cellsHippocampal progenitor cellsRole of FGFR1Glial progenitor cells
1997
Dlx-2 homeobox gene controls neuronal differentiation in primary cultures of developing basal ganglia
Ding M, Robel L, James A, Eisenstat D, Leckman J, Rubenstein J, Vaccarino F. Dlx-2 homeobox gene controls neuronal differentiation in primary cultures of developing basal ganglia. Journal Of Molecular Neuroscience 1997, 8: 93-113. PMID: 9188040, DOI: 10.1007/bf02736776.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody SpecificityAntisense Elements (Genetics)Basal GangliaCell DifferentiationCell DivisionCells, CulturedCytoskeletal ProteinsDNA-Binding ProteinsFemaleGene Expression Regulation, DevelopmentalGenes, HomeoboxHomeodomain ProteinsIn Situ HybridizationMicrotubule-Associated ProteinsNeuritesNeuronsPhenotypePregnancyRatsRhombencephalonRNA-Binding ProteinsTelencephalonTranscription FactorsConceptsGene productsNeuronal differentiationMicrotubule-associated protein MAP1BHomeodomain-containing genesDlx-2Homeobox genesNeuronal polarityCellular phenotypesNeuronal lineageProtein MAP1BPrimary culturesCellular localizationMitotic cycleGlial fibrillary acidic proteinGenesProteinCell proliferationDendrite outgrowthExpression of MAP2MAP2-positive dendritesNeuronal dendritesNeurofilament subunitsExpressionMAP2 expressionMRNA
1995
Fibroblast growth factor 2 increases Otx2 expression in precursor cells from mammalian telencephalon
Robel L, Ding M, James A, Lin X, Simeone A, Leckman J, Vaccarino F. Fibroblast growth factor 2 increases Otx2 expression in precursor cells from mammalian telencephalon. Journal Of Neuroscience 1995, 15: 7879-7891. PMID: 8613727, PMCID: PMC6577918, DOI: 10.1523/jneurosci.15-12-07879.1995.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell CountCells, CulturedDNA, ComplementaryDose-Response Relationship, DrugEmbryo, MammalianEmbryonic and Fetal DevelopmentFibroblast Growth Factor 2Gene ExpressionGenes, HomeoboxIntermediate Filament ProteinsMolecular ProbesMolecular Sequence DataNerve Tissue ProteinsNestinRatsStem CellsTelencephalonConceptsOtx2 expressionPrecursor cellsDifferent developmental stagesPopulation of neuroblastsRegulation of neurogenesisHomeobox genesEffects of FGF2Regulated fashionPostmitotic cellsPrimary culturesFibroblast growth factorBasic fibroblast growth factorMammalian telencephalonOtx2Developmental stagesMammalian forebrainDLX1FGF2Basic Fibroblast Growth Factor Increases the Number of Excitatory Neurons Containing Glutamate in the Cerebral Cortex
Vaccarino F, Schwartz M, Hartigan D, Leckman J. Basic Fibroblast Growth Factor Increases the Number of Excitatory Neurons Containing Glutamate in the Cerebral Cortex. Cerebral Cortex 1995, 5: 64-78. PMID: 7719131, DOI: 10.1093/cercor/5.1.64.Peer-Reviewed Original ResearchConceptsBasic fibroblast growth factorNerve growth factorGlutamate-containing neuronsCerebral cortexFibroblast growth factorGrowth factorAspartate-containing neuronsDifferent neurotransmitter phenotypesNumber of GABARatio of glutamateStem cellsNeurotransmitter phenotypeExcitatory neuronsInhibitory neuronsRat telencephalonVentricular zoneBFGF mRNAGABANeuronsCortexGlutamateDiffusible factorsThreefold increaseCellsFactors
1994
Excitatory amino acid receptors in glial progenitor cells: Molecular and functional properties
Gallo V, Patneau D, Mayer M, Vaccarino F. Excitatory amino acid receptors in glial progenitor cells: Molecular and functional properties. Glia 1994, 11: 94-101. PMID: 7927651, DOI: 10.1002/glia.440110204.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH Keywords6-Cyano-7-nitroquinoxaline-2,3-dioneAnimalsCells, CulturedCerebral CortexDNA-Binding ProteinsEarly Growth Response Protein 1Gene Expression RegulationGenes, Immediate-EarlyGlutamic AcidImmediate-Early ProteinsKainic AcidMembrane PotentialsNerve Tissue ProteinsNeurotoxinsN-MethylaspartateOligodendrogliaRatsReceptors, GlutamateStem CellsTranscription FactorsConceptsCG-4 cellsAMPA receptor antagonist CNQXWhole-cell patch-clamp recordingsExcitatory amino acid receptorsProgenitor cellsAmino acid receptorsRat cerebral cortexPatch-clamp recordingsGlial progenitor cellsGlutamate receptor subunitsAgonists L-glutamateGlutamate-gated channelsImmediate early gene NGFIAntagonist CNQXCerebral cortexGABA antibodyPrimary cell linesGlutamate receptorsTransient elevationAcid receptorsReceptor subunitsOligodendrocyte lineageOligodendrocyte progenitorsL-glutamateKainate
1993
Induction of immediate early genes by cyclic AMP in primary cultures of neurons from rat cerebral cortex
Vaccarino FM, Hayward MD, Le HN, Hartigan DJ, Duman RS, Nestler EJ. Induction of immediate early genes by cyclic AMP in primary cultures of neurons from rat cerebral cortex. Brain Research 1993, 19: 76-82. PMID: 8103187, DOI: 10.1016/0169-328x(93)90151-e.Peer-Reviewed Original ResearchMeSH Keywords2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepineAnimalsAnimals, NewbornBucladesineCells, CulturedCerebral CortexColforsinCyclic AMPDNA-Binding ProteinsDopamine AgentsEarly Growth Response Protein 1ErgolinesGene Expression RegulationGenes, fosGenes, junGlutamatesGlutamic AcidImmediate-Early ProteinsImmunohistochemistryKineticsNeurogliaNeuronsQuinpiroleRatsRNA, MessengerSecond Messenger SystemsTranscription FactorsVasoactive Intestinal PeptideConceptsVasoactive intestinal peptideRat cerebral cortexCerebral cortexExcitatory amino acid receptor antagonistsDibutyryl cAMPAmino acid receptor antagonistsPrimary culturesC-fosDihydropyridine-sensitive calcium channelsAcid receptor antagonistsIEG inductionCalcium-free mediumCAMP second messenger pathwayIEGs c-fosSKF 38393Immediate-early gene transcription factorsIntestinal peptideReceptor antagonistReceptor agonistSecond messenger pathwaysCalcium channelsDifferentiated neuronsBrief stimulationImmediate early genesIEG expression
1992
Differential induction of immediate early genes by excitatory amino acid receptor types in primary cultures of cortical and striatal neurons
Vaccarino F, Hayward M, Nestler E, Duman R, Tallman J. Differential induction of immediate early genes by excitatory amino acid receptor types in primary cultures of cortical and striatal neurons. Brain Research 1992, 12: 233-241. PMID: 1347632, DOI: 10.1016/0169-328x(92)90089-t.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine6-Cyano-7-nitroquinoxaline-2,3-dioneAnimalsAnimals, NewbornBlotting, NorthernCells, CulturedCerebral CortexCorpus StriatumDNA-Binding ProteinsEarly Growth Response Protein 1Gene Expression RegulationGenes, fosGenes, junGenes, RegulatorGlutamatesGlutamic AcidGlycineImmediate-Early ProteinsIsoquinolinesNeuronsPiperazinesProtein Kinase InhibitorsProto-OncogenesQuinoxalinesQuisqualic AcidRatsReceptors, AMPAReceptors, NeurotransmitterReceptors, N-Methyl-D-AspartateRNA, MessengerSulfonamidesTranscription FactorsConceptsProtein kinase C inhibitor HNGFI-A mRNAC-fosImmediate early genesPrimary culturesC-JunEarly genesGene mRNAN-methyl-D-aspartate receptor typeExcitatory amino acid receptor typesReceptor typesInhibitor HHydroxy-5-methylisoxazolepropionic acidExcitatory amino acid glutamateIEG expressionTransmembrane calcium influxDifferential inductionNMDA receptor channelsNMDA receptor activationAmino acid glutamateMRNAReceptor activationImmediate early gene mRNAsReceptor channelsCerebral cortex
1991
Modulation of Protein Kinase C Translocation by Excitatory and Inhibitory Amino Acids in Primary Cultures of Neurons
Vaccarino F, Liljequist S, Tallman J. Modulation of Protein Kinase C Translocation by Excitatory and Inhibitory Amino Acids in Primary Cultures of Neurons. Journal Of Neurochemistry 1991, 57: 391-396. PMID: 1649249, DOI: 10.1111/j.1471-4159.1991.tb03765.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino AcidsAnimalsAnimals, NewbornBicucullineCell MembraneCells, CulturedCerebral CortexCorpus StriatumDizocilpine MaleateGlutamatesKineticsNeuronsOxadiazolesPhorbol 12,13-DibutyrateProtein Kinase CRatsRats, Inbred StrainsReceptors, AMPAReceptors, NeurotransmitterReceptors, N-Methyl-D-AspartateTetrodotoxinConceptsProtein kinase CPKC translocationAmino acidsExtracellular mediumProtein kinase C translocationAmino acid stimulationPrimary culturesKinase CC translocationExcitatory amino acid receptorsTranslocationExcitatory amino acid stimulationImine hydrogen maleateExcitatory amino acidsAmino acid receptorsRat cerebral cortexInhibitory amino acidsNMDA receptor antagonistAbsence of calciumAcid receptorsPhysiological concentrationsCerebral cortexMetabotropic receptorsReceptor antagonistAMPA receptors
1990
Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function
Eva C, Bovolin P, Balzac F, Botta C, Ricci Gamalero S, Vaccarino F. Primary cultures of corticostriatal cells from newborn rats: A model to study muscarinic receptor subtypes regulation and function. Journal Of Molecular Neuroscience 1990, 2: 143. PMID: 2177349, DOI: 10.1007/bf02896839.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAdenylyl CyclasesAnimalsAnimals, NewbornCarbacholCells, CulturedCerebral CortexCholine O-AcetyltransferaseCorpus StriatumKineticsNeuronsPhosphatidylinositolsPolymerase Chain ReactionQuinuclidinyl BenzilateRadioligand AssayRatsReceptors, MuscarinicRNA, MessengerSecond Messenger SystemsConceptsAmount of acetylcholineDensity of mAChRsAdult rat cortexCholinergic receptorsRat cortexNeuronal culturesCholine acetyltransferase-immunoreactive neuronsPrimary culturesMuscarinic cholinergic receptorsTranscriptase-polymerase chain reaction techniqueReverse transcriptase-polymerase chain reaction techniqueNewborn rat brainAdenylate cyclase activityCorticostriatal cellsCorticostriatal neuronsImmunoreactive neuronsSubtype mRNAsChain reaction techniqueMuscarinic antagonistCholinergic transmissionMuscarinic agonistsSecond messenger systemsPostsynaptic componentsNewborn ratsRat brain
1988
Subsets of GABAergic neurons in dissociated cell cultures of neonatal rat cerebral cortex show co-localization with specific modulator peptides.
Alho H, Ferrarese C, Vicini S, Vaccarino F. Subsets of GABAergic neurons in dissociated cell cultures of neonatal rat cerebral cortex show co-localization with specific modulator peptides. Brain Research 1988, 467: 193-204. PMID: 3378169, DOI: 10.1016/0165-3806(88)90023-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornCarrier ProteinsCells, CulturedCerebral CortexDiazepam Binding InhibitorElectrophysiologyGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidGlutamate DecarboxylaseMembrane ProteinsMembrane Transport ProteinsNerve Tissue ProteinsNeuronsNeuropeptidesOrganic Anion TransportersPeptide FragmentsRatsConceptsGlutamic acid decarboxylaseNeuropeptide YGABAergic propertiesCerebral cortexGAD immunoreactivityGABA-modulinNeonatal cortical neuronsPrimary culturesInhibitory synaptic currentsDouble-labeling studiesBenzodiazepine recognition sitesGABA receptor modulatorsUseful experimental modelDays of cultureNeuroregulatory peptideGABAergic neuronsGABAergic neurotransmissionSpontaneous excitatoryNeonatal ratsCortical neuronsReceptor modulatorsLower incidenceTyrosine hydroxylaseSynaptic currentsCultured neurons
1987
Ganglioside inhibition of glutamate-mediated protein kinase C translocation in primary cultures of cerebellar neurons.
Vaccarino F, Guidotti A, Costa E. Ganglioside inhibition of glutamate-mediated protein kinase C translocation in primary cultures of cerebellar neurons. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 8707-8711. PMID: 2825205, PMCID: PMC299615, DOI: 10.1073/pnas.84.23.8707.Peer-Reviewed Original ResearchConceptsGranule cellsProtein kinase C translocationExcitatory amino acid neurotransmitter receptorsAmino acid neurotransmitter receptorsPrimary culturesGlutamate-mediated Ca2Cerebellar granule cellsHigh affinity recognition sitesPKC translocationNeurotransmitter receptorsRemoval of Ca2Cerebellar neuronsActivation of PKCC translocationNeuronal membranesGanglioside inhibitionRedistribution of PKCIncubation mediumGlutamateCa2PKCActivationSubcellular Location and Neuronal Release of Diazepam Binding Inhibitor
Ferrarese C, Vaccarino F, Alho H, Mellstrom B, Costa E, Guidotti A. Subcellular Location and Neuronal Release of Diazepam Binding Inhibitor. Journal Of Neurochemistry 1987, 48: 1093-1102. PMID: 3819722, DOI: 10.1111/j.1471-4159.1987.tb05632.x.Peer-Reviewed Original ResearchConceptsDBI-like immunoreactivitySlices of hypothalamusCerebral cortical neuronsGamma-aminobutyric acid receptorsRat brain neuronsDiazepam binding inhibitorAllosteric modulatory sitesVeratridine depolarizationNeuronal releaseCerebral cortexMicroM tetrodotoxinSlices of liverPeripheral organsCNS neuronsCortical neuronsBinding of benzodiazepinesCortical astrocytesBrain neuronsNeuromodulatory substancesRat brainSynaptosomal lysatesModulatory siteMet5-enkephalinAcid receptorsNeuronsCoexistence of GABA receptors and GABA-modulin in primary cultures of rat cerebellar granule cells
Vaccarino FM, Alho H, Santi MR, Guidotti A. Coexistence of GABA receptors and GABA-modulin in primary cultures of rat cerebellar granule cells. Journal Of Neuroscience 1987, 7: 65-76. PMID: 3027277, PMCID: PMC6568852, DOI: 10.1523/jneurosci.07-01-00065.1987.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCells, CulturedCerebellumFlunitrazepamFluorescent Antibody TechniqueGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidHistocytochemistryMembrane ProteinsMembrane Transport ProteinsMuscimolNerve Tissue ProteinsOrganic Anion TransportersRadioimmunoassayRatsReceptors, GABA-ASynaptosomesConceptsRat brain synaptosomesGranule cell culturesBrain synaptosomesGranule cellsGABA-modulinPrimary culturesCerebellar granule cell culturesRat cerebellar granule cellsPurkinje cell dendritesGranular cell layerGABA receptor complexGABA recognition siteCerebellar granule cellsGABAergic neuronsGABAergic interneuronsCell dendritesGlial cellsGABA receptorsIntact cell monolayersCell membrane preparationsCell culturesCerebellar interneuronsCell bodiesSpecific antibodiesSynaptosomes