2019
UAF1 DNA Binding Activity Is Critical for RAD51-Mediated Homologous DNA Pairing
Liang F, Miller A, Tang C, Sung P, Kupfer G. UAF1 DNA Binding Activity Is Critical for RAD51-Mediated Homologous DNA Pairing. Blood 2019, 134: 2497. DOI: 10.1182/blood-2019-130435.Peer-Reviewed Original ResearchHomologous DNA pairingHomologous recombinationDNA binding activityDNA repairDNA bindingDNA pairingHomologous DNAFanconi anemia DNA repair pathwayHR efficiencyFA core complexDNA damageDNA damage sensitivityDNA damage resistanceDNA-binding mutantSynaptic complex assemblyBinding activityE3 ligase activityDNA damage repairDNA repair pathwaysPresynaptic complex formationFANCD2 deubiquitinationGenome maintenanceComplex formationDeubiquitinase complexMutant proteinsChromatin‐bound cGAS is an inhibitor of DNA repair and hence accelerates genome destabilization and cell death
Jiang H, Xue X, Panda S, Kawale A, Hooy RM, Liang F, Sohn J, Sung P, Gekara NO. Chromatin‐bound cGAS is an inhibitor of DNA repair and hence accelerates genome destabilization and cell death. The EMBO Journal 2019, 38: e102718. PMID: 31544964, PMCID: PMC6826206, DOI: 10.15252/embj.2019102718.Peer-Reviewed Original ResearchConceptsCyclic GMP-AMP synthaseHomologous recombinationDNA repairGenome destabilizationCell deathChromatin-binding proteinsGMP-AMP synthaseSsDNA filamentsGenome integrityGenomic stressInnate immune sensorsHR repairChromosomal rearrangementsStrand invasionRegulatory mechanismsUnknown roleCytosolic innate immune sensorsCell survivalTemplate dsDNAImmune sensorsBone marrow cellsMicronuclei generationOutcome of infectionMarrow cellsInnate immune activation
2016
The Role of UAF1 in the Fanconi Anemia Pathway Regulation of Homologous Recombination-Mediated Genome Maintenance
Liang F, Longerich S, Tang C, Buzovestsky O, Xiong Y, Maranon D, Wiese C, Miller A, Sung P, Kupfer G. The Role of UAF1 in the Fanconi Anemia Pathway Regulation of Homologous Recombination-Mediated Genome Maintenance. Blood 2016, 128: 1041. DOI: 10.1182/blood.v128.22.1041.1041.Peer-Reviewed Original ResearchHomologous recombinationGenome maintenanceDNA damageFA DNA repair pathwayCancer-prone genetic diseasesFanconi anemiaDNA damage hypersensitivityFunctional synergySynaptic complex assemblyProtein complex formationRad51 presynaptic filamentD-loop reactionDNA repair functionDNA damage repairDNA binding activityCell linesFANCD2 ubiquitinationGenome stabilityComplex formationRAD51 recombinaseHuman cell linesBinds DNAPresynaptic filamentRecombinase RAD51UAF1Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex
Liang F, Longerich S, Miller AS, Tang C, Buzovetsky O, Xiong Y, Maranon DG, Wiese C, Kupfer GM, Sung P. Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex. Cell Reports 2016, 15: 2118-2126. PMID: 27239033, PMCID: PMC5381662, DOI: 10.1016/j.celrep.2016.05.007.Peer-Reviewed Original ResearchConceptsHomologous recombinationFanconi anemia DNA damage response pathwayDNA damage response pathwaySmall ubiquitin-like modifierDNA damage hypersensitivityHomologous DNA pairingSUMO-interacting motifDamage response pathwayUbiquitin-like modifierFANCD2 ubiquitinationGenome maintenanceComplex formationNucleoprotein intermediatesRAD51 recombinaseDNA pairingProtein complexesBinds DNAAccessory factorsResponse pathwaysHomologous DNADNA repairLike domainUAF1RAD51RAD51AP1
2015
NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability
Parplys AC, Zhao W, Sharma N, Groesser T, Liang F, Maranon DG, Leung SG, Grundt K, Dray E, Idate R, Østvold AC, Schild D, Sung P, Wiese C. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Research 2015, 43: 9817-9834. PMID: 26323318, PMCID: PMC4787752, DOI: 10.1093/nar/gkv859.Peer-Reviewed Original ResearchConceptsHomologous recombinationDNA replication fork progressionChromatin-associated proteinsVertebrate-specific proteinReplication fork progressionDNA damage responseDNA repair pathwaysNew replication originsNumber of proteinsExtensive sequence homologyImpairs DNA repairGenome stabilityLimited functional dataChromosome stabilityFork progressionDamage responseReplication originsHR pathwayPhenotypic effectsDNA repairRepair pathwaysSequence homologyTumor suppressionSame DNANUCKS1