2020
The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair
Liang F, Miller AS, Tang C, Maranon D, Williamson EA, Hromas R, Wiese C, Zhao W, Sung P, Kupfer GM. The DNA-binding activity of USP1-associated factor 1 is required for efficient RAD51-mediated homologous DNA pairing and homology-directed DNA repair. Journal Of Biological Chemistry 2020, 295: 8186-8194. PMID: 32350107, PMCID: PMC7294083, DOI: 10.1074/jbc.ra120.013714.Peer-Reviewed Original ResearchConceptsHomologous DNA pairingDNA-binding activityDNA pairingDNA repairDNA bindingFanconi anemia complementation group D2 proteinDamage repairHomology-directed DNA repairRAD51 recombinase activityFanconi anemia pathwayDNA damage sensitivityChromosome damage repairHR-mediated DNA repairDNA damage responseDNA damage repairHomologous recombination pathwayFactor 1FANCD2 deubiquitinationRole of DNAEfficient RAD51Deubiquitinase complexRecombinase RAD51Damage responseUAF1D2 protein
2019
UAF1 DNA Binding Activity Is Critical for RAD51-Mediated Homologous DNA Pairing
Liang F, Miller A, Tang C, Sung P, Kupfer G. UAF1 DNA Binding Activity Is Critical for RAD51-Mediated Homologous DNA Pairing. Blood 2019, 134: 2497. DOI: 10.1182/blood-2019-130435.Peer-Reviewed Original ResearchHomologous DNA pairingHomologous recombinationDNA binding activityDNA repairDNA bindingDNA pairingHomologous DNAFanconi anemia DNA repair pathwayHR efficiencyFA core complexDNA damageDNA damage sensitivityDNA damage resistanceDNA-binding mutantSynaptic complex assemblyBinding activityE3 ligase activityDNA damage repairDNA repair pathwaysPresynaptic complex formationFANCD2 deubiquitinationGenome maintenanceComplex formationDeubiquitinase complexMutant proteinsDNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
Liang F, Miller AS, Longerich S, Tang C, Maranon D, Williamson EA, Hromas R, Wiese C, Kupfer GM, Sung P. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature Communications 2019, 10: 2849. PMID: 31253762, PMCID: PMC6599204, DOI: 10.1038/s41467-019-10408-5.Peer-Reviewed Original ResearchConceptsFANCD2 deubiquitinationDNA damage response pathwayFanconi anemiaUSP1-UAF1 complexDNA damage toleranceDNA damage responseDamage response pathwayImportance of DNAGenome repairDamage responseResponse pathwaysUAF1DNA requirementsCellular settingsFA pathwayDNA bindingDownstream eventsDeubiquitinationRAD51AP1DNA crosslinksBiochemical systemsBone marrow failureChromosomal lesionsMultigenic diseasesMultifaceted role