Featured Publications
Modulation of VEGF receptor 2 signaling by protein phosphatases
Corti F, Simons M. Modulation of VEGF receptor 2 signaling by protein phosphatases. Pharmacological Research 2016, 115: 107-123. PMID: 27888154, PMCID: PMC5205541, DOI: 10.1016/j.phrs.2016.11.022.Peer-Reviewed Original ResearchConceptsProtein phosphatasePhosphorylation of serineVascular endothelial growth factor receptor 2 signalingSignal transduction cascadePrecise biological roleSpecific signaling pathwaysKinase biologyEukaryotic cellsSignal terminatorRegulatory subunitPositive regulatorTransduction cascadePhosphorylation stateBiological roleContext of cancerParticular proteinDifferent proteinsGenome sequencingSignaling pathwaysVEGF receptor 2Receptor 2 signalingVEGF signalsProteinPhosphataseGenetic modelsSyndecan 4 is required for endothelial alignment in flow and atheroprotective signaling
Baeyens N, Mulligan-Kehoe MJ, Corti F, Simon DD, Ross TD, Rhodes JM, Wang TZ, Mejean CO, Simons M, Humphrey J, Schwartz MA. Syndecan 4 is required for endothelial alignment in flow and atheroprotective signaling. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 17308-17313. PMID: 25404299, PMCID: PMC4260558, DOI: 10.1073/pnas.1413725111.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtherosclerosisBlotting, WesternCells, CulturedEndothelial CellsFemaleHuman Umbilical Vein Endothelial CellsHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMaleMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalNF-kappa BReverse Transcriptase Polymerase Chain ReactionRNA InterferenceSignal TransductionStress, MechanicalSyndecan-4Vascular Endothelial Growth Factor Receptor-2ConceptsHuman umbilical vein endothelial cellsNF-κBProinflammatory NF-κBAtherosclerotic plaque burdenKruppel-like factor 2Umbilical vein endothelial cellsVEGF receptor 2Appearance of plaquesVein endothelial cellsHypercholesterolemic micePlaque burdenAntiinflammatory pathwayThoracic aortaReceptor 2Endothelial cellsEndothelial alignmentFlow correlatesCausal roleAtherosclerosisFactor 2MiceCyclic stretchLocalization correlatesActivationSyndecan-4The Syndecan-4/Protein Kinase Cα Pathway Mediates Prostaglandin E2-induced Extracellular Regulated Kinase (ERK) Activation in Endothelial Cells and Angiogenesis in Vivo *
Corti F, Finetti F, Ziche M, Simons M. The Syndecan-4/Protein Kinase Cα Pathway Mediates Prostaglandin E2-induced Extracellular Regulated Kinase (ERK) Activation in Endothelial Cells and Angiogenesis in Vivo *. Journal Of Biological Chemistry 2013, 288: 12712-12721. PMID: 23525101, PMCID: PMC3642317, DOI: 10.1074/jbc.m113.452383.Peer-Reviewed Original ResearchConceptsERK activationExtracellular-regulated kinase activationProteoglycan syndecan-4Protein kinase CαEndothelial cell migrationKinase activationEndothelial cellsSyndecan-4Full rescueCord formationCell migrationActivation of angiogenesisMatrigel assaysTransductionAngiogenic responseAngiogenesisActivationTumor growthImportant roleCellsMutantsMain mediatorVivoSDC4PKCα
2020
Amyloid-β Precursor Protein APP Down-Regulation Alters Actin Cytoskeleton-Interacting Proteins in Endothelial Cells
Ristori E, Cicaloni V, Salvini L, Tinti L, Tinti C, Simons M, Corti F, Donnini S, Ziche M. Amyloid-β Precursor Protein APP Down-Regulation Alters Actin Cytoskeleton-Interacting Proteins in Endothelial Cells. Cells 2020, 9: 2506. PMID: 33228083, PMCID: PMC7699411, DOI: 10.3390/cells9112506.Peer-Reviewed Original ResearchConceptsAmyloid-β precursor proteinCerebral amyloid angiopathyUbiquitous membrane proteinsFocal adhesion stabilityEndothelial cellsMajor cellular targetInteracting proteinActin cytoskeletonProteomic approachMembrane proteinsAlzheimer's diseaseMolecular mechanismsCellular responsesCellular targetsPhysiological roleRole of APPEndothelial cell proliferationPrecursor proteinCell proliferationNormal endothelial functionProteinNeuronal tissueGrowth factorExogenous stimuliExpression
2018
Bradykinin B2 Receptor Contributes to Inflammatory Responses in Human Endothelial Cells by the Transactivation of the Fibroblast Growth Factor Receptor FGFR-1
Terzuoli E, Corti F, Nannelli G, Giachetti A, Donnini S, Ziche M. Bradykinin B2 Receptor Contributes to Inflammatory Responses in Human Endothelial Cells by the Transactivation of the Fibroblast Growth Factor Receptor FGFR-1. International Journal Of Molecular Sciences 2018, 19: 2638. PMID: 30200598, PMCID: PMC6163484, DOI: 10.3390/ijms19092638.Peer-Reviewed Original ResearchConceptsFibroblast growth factor receptor 1Human umbilical vein endothelial cellsCell permeabilityEndothelial cellsFGFR-1 phosphorylationEndothelial cell permeabilityFGF-2 signalingFGF-2 pathwaysFGF-2/FGFRFGFR-1 inhibitorsC-SrcGrowth factor receptor 1Umbilical vein endothelial cellsDownstream signalingHuman endothelial cellsGrowth factor 2Vein endothelial cellsFactor receptor 1Cell migrationFGF-2 upregulationAmplification loopSignalingFactor 2Angiogenic disordersBradykinin B2 receptorPeptides derived from the histidine–proline rich glycoprotein bind copper ions and exhibit anti-angiogenic properties
Magrì A, Grasso G, Corti F, Finetti F, Greco V, Santoro AM, Sciuto S, La Mendola D, Morbidelli L, Rizzarelli E. Peptides derived from the histidine–proline rich glycoprotein bind copper ions and exhibit anti-angiogenic properties. Dalton Transactions 2018, 47: 9492-9503. PMID: 29963662, DOI: 10.1039/c8dt01560k.Peer-Reviewed Original ResearchConceptsElectron paramagnetic resonanceCircular dichroismSpray ionization mass spectrometryPotential drug delivery systemElectron spray ionization mass spectrometryMeans of potentiometryIonization mass spectrometryDrug delivery systemsAmidic bondCopper ionsRole of copperParamagnetic resonanceMass spectrometryComplex speciesTrehalose derivativesProdrug systemEnzymatic degradationDelivery systemCopperPeptidesPotentiometryBondsUVSpectrometryDichroism
2016
Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development
Wang Y, Baeyens N, Corti F, Tanaka K, Fang JS, Zhang J, Jin Y, Coon B, Hirschi KK, Schwartz MA, Simons M. Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development. Development 2016, 143: 4441-4451. PMID: 27789626, PMCID: PMC5201046, DOI: 10.1242/dev.140129.Peer-Reviewed Original ResearchConceptsLymphatic endothelial cellsPlanar cell polarity protein Vangl2Lymphatic vessel remodelingMouse embryonic developmentHuman lymphatic endothelial cellsVangl2 overexpressionVangl2 expressionEmbryonic developmentValve morphogenesisEndothelial cellsVasculature developmentSyndecan-4Lymphatic vasculatureFluid shear stressSDC4Double knockout miceMice resultsHigh expressionVessel remodelingLymphatic vesselsExpressionVangl2RemodelingCellsMorphogenesis
2014
E‐NTPDase1/CD39 modulates renin release from heart mast cells during ischemia/reperfusion: a novel cardioprotective role
Aldi S, Marino A, Tomita K, Corti F, Anand R, Olson KE, Marcus AJ, Levi R. E‐NTPDase1/CD39 modulates renin release from heart mast cells during ischemia/reperfusion: a novel cardioprotective role. The FASEB Journal 2014, 29: 61-69. PMID: 25318477, PMCID: PMC4285537, DOI: 10.1096/fj.14-261867.Peer-Reviewed Original ResearchConceptsRenin releaseRenin-angiotensin systemMast cellsVentricular fibrillationRAS activationHMC-1Murine bone marrow-derived mast cellsLocal renin-angiotensin systemBone marrow-derived mast cellsCardiac mast cellsNovel cardioprotective roleMarrow-derived mast cellsPurinergic P2X7 receptorIschemia/reperfusionHeart mast cellsCD39 inhibitionCD39 overexpressionP2X7R blockadeNorepinephrine overflowCardioprotective effectsCardioprotective roleP2X7 receptorCD39Pharmacological inhibitionATP release
2013
Mitochondrial aldehyde dehydrogenase-2 activation prevents β-amyloid-induced endothelial cell dysfunction and restores angiogenesis
Solito R, Corti F, Chen C, Mochly-Rosen D, Giachetti A, Ziche M, Donnini S. Mitochondrial aldehyde dehydrogenase-2 activation prevents β-amyloid-induced endothelial cell dysfunction and restores angiogenesis. Journal Of Cell Science 2013, 126: 1952-1961. PMID: 23447675, PMCID: PMC3666252, DOI: 10.1242/jcs.117184.Peer-Reviewed Original ResearchMeSH KeywordsAldehyde DehydrogenaseAldehyde Dehydrogenase, MitochondrialAmyloid beta-PeptidesBenzamidesBenzodioxolesBeta CateninEnzyme ActivationEnzyme ActivatorsHuman Umbilical Vein Endothelial CellsHumansMembrane PotentialsMitochondrial ProteinsNeovascularization, PhysiologicOxidative StressPeptide FragmentsPhosphorylationReactive Oxygen Species
2010
Aldehyde Dehydrogenase Activation Prevents Reperfusion Arrhythmias by Inhibiting Local Renin Release From Cardiac Mast Cells
Koda K, Salazar-Rodriguez M, Corti F, Chan NY, Estephan R, Silver RB, Mochly-Rosen D, Levi R. Aldehyde Dehydrogenase Activation Prevents Reperfusion Arrhythmias by Inhibiting Local Renin Release From Cardiac Mast Cells. Circulation 2010, 122: 771-781. PMID: 20697027, PMCID: PMC2927811, DOI: 10.1161/circulationaha.110.952481.Peer-Reviewed Original ResearchMeSH KeywordsAldehyde DehydrogenaseAnimalsArrhythmias, CardiacCell DegranulationCell Line, TumorEnzyme ActivationGuinea PigsHumansIschemic Preconditioning, MyocardialMaleMast CellsMyocardial Reperfusion InjuryMyocytes, CardiacProtein Kinase C-epsilonReceptor, Adenosine A2BReceptor, Adenosine A3ReninRenin-Angiotensin SystemConceptsCardiac mast cellsAnti-RA effectRenin-angiotensin systemAldehyde dehydrogenase type 2Ischemic preconditioningIschemia/reperfusionMast cellsRenin releaseALDH2 activationReperfusion arrhythmiasNorepinephrine releaseRAS activationHMC-1Local renin-angiotensin systemBlockade of adenosineLocal RAS activationClassic ischemic preconditioningDehydrogenase type 2New therapeutic targetsCultured mast cellsPKCepsilon inhibitionFibrillation durationMyocardial ischemiaVentricular tachycardiaHearts ex vivo
2008
Dutch and arctic mutant peptides of β amyloid1–40 differentially affect the FGF-2 pathway in brain endothelium
Solito R, Corti F, Fossati S, Mezhericher E, Donnini S, Ghiso J, Giachetti A, Rostagno A, Ziche M. Dutch and arctic mutant peptides of β amyloid1–40 differentially affect the FGF-2 pathway in brain endothelium. Experimental Cell Research 2008, 315: 385-395. PMID: 19061884, PMCID: PMC2764262, DOI: 10.1016/j.yexcr.2008.11.002.Peer-Reviewed Original ResearchConceptsAnti-angiogenic profileDegree of tropismCerebral amyloid angiopathyFGF-2 expressionFGF-2 receptorFibroblast growth factorEndothelial cell proliferationMicrovessel remodelingAmyloid angiopathyBrain endotheliumΒ-amyloid1Brain vesselsInduction of apoptosisAbeta variantsCerebral ECsFGF-2 pathwaysG variantE22GGrowth factorWT peptideFGFR-1Cell proliferationFGF-2Hereditary phenotypesDifferential effects
2007
Molecular Basis of Branched Peptides Resistance to Enzyme Proteolysis
Falciani C, Lozzi L, Pini A, Corti F, Fabbrini M, Bernini A, Lelli B, Niccolai N, Bracci L. Molecular Basis of Branched Peptides Resistance to Enzyme Proteolysis. Chemical Biology & Drug Design 2007, 69: 216-221. PMID: 17441908, DOI: 10.1111/j.1747-0285.2007.00487.x.Peer-Reviewed Original ResearchConceptsForm of dendrimersDifferent bioactive peptidesStructure-based hypothesesMass spectrometryUnmodified peptidesMultimeric peptidesProteolytic stabilityBioactive peptidesPressure liquid chromatographyLiquid chromatographyHigh-pressure liquid chromatographyLinear moleculesPeptide copiesEnzyme proteolysisProteolytic resistanceHuman plasmaSame peptideSynthetic peptidesPeptidesDendrimersPeptide resistanceSpectrometryMultimericityChromatographyMolecules