Trimeric Subunit Stoichiometry of the Glutamate Transporters from Bacillus caldotenax and Bacillus stearothermophilus †
Yernool D, Boudker O, Folta-Stogniew E, Gouaux E. Trimeric Subunit Stoichiometry of the Glutamate Transporters from Bacillus caldotenax and Bacillus stearothermophilus †. Biochemistry 2003, 42: 12981-12988. PMID: 14596613, DOI: 10.1021/bi030161q.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid Transport System X-AGBacillusChromatography, GelCross-Linking ReagentsElectrophoresis, Polyacrylamide GelGenetic VectorsGeobacillus stearothermophilusGlutaralLasersLiposomesMolecular Sequence DataMolecular WeightProtein SubunitsProtein TransportScattering, RadiationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrophotometry, UltravioletConceptsSubunit stoichiometryBacillus caldotenaxMembrane proteinsPolytopic integral membrane proteinPrimary structureAmino acid sequence relationshipsGlutamate transportersBacillus stearothermophilusKingdoms of lifeIntegral membrane proteinsNeutral amino acid transporterTrimeric quaternary structureNative molecular massDetergent-solubilized stateAmino acid transportersSize exclusion chromatographyB. caldotenaxEukaryotic organismsHigh-affinity glutamate transportersEukaryotic transportersQuaternary organizationAqueous solutionNutrient uptakeLife formsDetergent micelles