2006
Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors
Folta-Stogniew E. Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors. Methods In Molecular Biology 2006, 328: 97-112. PMID: 16785643, DOI: 10.1385/1-59745-026-x:97.Peer-Reviewed Original ResearchMeSH KeywordsCalibrationCell MembraneChromatographyDetergentsGlycoproteinsIonsLigandsLightProteinsScattering, RadiationSpectrophotometryUltraviolet RaysConceptsSize exclusion chromatographyRefractive indexMolar massStatic laser lightLaser lightOligomeric stateRefractive index detectorProtein-detergent complexesLight scatteringIndex detectorUltraviolet detectionDetectorFractionation stepsNon-ionic detergentAnomalous behaviorGlycosylated proteinsChromatographyNative proteinScatteringMassPolypeptide chainStateRI analysisRI approachUniversal approach
2003
Trimeric Subunit Stoichiometry of the Glutamate Transporters from Bacillus caldotenax and Bacillus stearothermophilus †
Yernool D, Boudker O, Folta-Stogniew E, Gouaux E. Trimeric Subunit Stoichiometry of the Glutamate Transporters from Bacillus caldotenax and Bacillus stearothermophilus †. Biochemistry 2003, 42: 12981-12988. PMID: 14596613, DOI: 10.1021/bi030161q.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAmino Acid Transport System X-AGBacillusChromatography, GelCross-Linking ReagentsElectrophoresis, Polyacrylamide GelGenetic VectorsGeobacillus stearothermophilusGlutaralLasersLiposomesMolecular Sequence DataMolecular WeightProtein SubunitsProtein TransportScattering, RadiationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSpectrophotometry, UltravioletConceptsSubunit stoichiometryBacillus caldotenaxMembrane proteinsPolytopic integral membrane proteinPrimary structureAmino acid sequence relationshipsGlutamate transportersBacillus stearothermophilusKingdoms of lifeIntegral membrane proteinsNeutral amino acid transporterTrimeric quaternary structureNative molecular massDetergent-solubilized stateAmino acid transportersSize exclusion chromatographyB. caldotenaxEukaryotic organismsHigh-affinity glutamate transportersEukaryotic transportersQuaternary organizationAqueous solutionNutrient uptakeLife formsDetergent micelles