Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors
Folta-Stogniew E. Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors. Methods In Molecular Biology 2006, 328: 97-112. PMID: 16785643, DOI: 10.1385/1-59745-026-x:97.Peer-Reviewed Original ResearchConceptsSize exclusion chromatographyRefractive indexMolar massStatic laser lightLaser lightOligomeric stateRefractive index detectorProtein-detergent complexesLight scatteringIndex detectorUltraviolet detectionDetectorFractionation stepsNon-ionic detergentAnomalous behaviorGlycosylated proteinsChromatographyNative proteinScatteringMassPolypeptide chainStateRI analysisRI approachUniversal approach