2015
Estrogen in prefrontal cortex blocks stress-induced cognitive impairments in female rats
Yuen EY, Wei J, Yan Z. Estrogen in prefrontal cortex blocks stress-induced cognitive impairments in female rats. The Journal Of Steroid Biochemistry And Molecular Biology 2015, 160: 221-226. PMID: 26321384, PMCID: PMC4769981, DOI: 10.1016/j.jsbmb.2015.08.028.Peer-Reviewed Original ResearchConceptsPrefrontal cortexPFC of femalesStress-induced cognitive impairmentGlutamate receptor surface expressionSame stress paradigmCognitive behaviorMemory impairmentCognitive impairmentGlutamatergic deficitsStress paradigmFemale ratsLevels of aromataseContribution of estrogenNormal synaptic functionImpairmentReceptor surface expressionBiosynthesis of estrogensGlutamatergic transmissionPyramidal neuronsMale ratsEstrogen receptorCognitionHuman studiesProtective roleSynaptic function
2014
Synergistic Regulation of Glutamatergic Transmission by Serotonin and Norepinephrine Reuptake Inhibitors in Prefrontal Cortical Neurons*
Yuen EY, Qin L, Wei J, Liu W, Liu A, Yan Z. Synergistic Regulation of Glutamatergic Transmission by Serotonin and Norepinephrine Reuptake Inhibitors in Prefrontal Cortical Neurons*. Journal Of Biological Chemistry 2014, 289: 25177-25185. PMID: 25056951, PMCID: PMC4155681, DOI: 10.1074/jbc.m114.567610.Peer-Reviewed Original ResearchMeSH KeywordsAdrenergic Uptake InhibitorsAnimalsBicucullineBlotting, WesternCarrier ProteinsCells, CulturedDesipramineDrug SynergismDynaminsEndocytosisExcitatory Postsynaptic PotentialsFluoxetineP38 Mitogen-Activated Protein KinasesPatch-Clamp TechniquesPrefrontal CortexPyramidal CellsRab5 GTP-Binding ProteinsRatsReceptor, Serotonin, 5-HT1AReceptors, Adrenergic, alpha-2Receptors, AMPASelective Serotonin Reuptake InhibitorsSignal TransductionSynaptic TransmissionConceptsNorepinephrine reuptake inhibitorsExcitatory postsynaptic currentsΑ2-adrenergic receptorsReuptake inhibitorsGlutamatergic transmissionPostsynaptic currentsCortical neuronsAMPAR-mediated excitatory postsynaptic currentsG proteinsPrefrontal cortex pyramidal neuronsDynamin-mediated endocytosisSelective reuptake inhibitorsProtein α subunitsCortex pyramidal neuronsPrefrontal cortical neuronsP38 kinase activityAMPAR surface expressionGood therapeutic effectG-protein modulatorsPyramidal neuronsClinical studiesTherapeutic effectMonoamine systemsAMPA receptorsKinase activity
2013
Restoration of Glutamatergic Transmission by Dopamine D4 Receptors in Stressed Animals*
Yuen EY, Zhong P, Li X, Wei J, Yan Z. Restoration of Glutamatergic Transmission by Dopamine D4 Receptors in Stressed Animals*. Journal Of Biological Chemistry 2013, 288: 26112-26120. PMID: 23884421, PMCID: PMC3764814, DOI: 10.1074/jbc.m112.396648.Peer-Reviewed Original ResearchConceptsD4 receptorsDopamine D4 receptorExcitatory transmissionGlutamatergic transmissionPrefrontal cortexAMPA receptor-mediated synaptic transmissionReceptor-mediated synaptic transmissionCalcium/calmodulin kinase II activityEffects of D4PFC pyramidal neuronsCalmodulin kinase II activityKey brain regionsAttention deficit hyperactivity disorderBi-directional regulationDeficit hyperactivity disorderPyramidal neuronsCortical excitabilityDopaminergic inputSynaptic actionsSynaptic transmissionPotent reductionAMPA receptorsKinase II activityStressed animalsMaladaptive changesEstrogen protects against the detrimental effects of repeated stress on glutamatergic transmission and cognition
Wei J, Yuen EY, Liu W, Li X, Zhong P, Karatsoreos IN, McEwen BS, Yan Z. Estrogen protects against the detrimental effects of repeated stress on glutamatergic transmission and cognition. Molecular Psychiatry 2013, 19: 588-598. PMID: 23835908, DOI: 10.1038/mp.2013.83.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAromataseAromatase InhibitorsCognitionEstradiolEstrogensFemaleGlutamic AcidMaleMemory DisordersPrefrontal CortexPyramidal CellsRats, Sprague-DawleyReceptors, EstrogenReceptors, GlutamateRecognition, PsychologyRNA, MessengerSex CharacteristicsStress, PsychologicalSynaptic TransmissionConceptsTemporal order recognition memoryGlutamatergic transmissionPrefrontal cortexStressed malesPFC of femalesNormal glutamatergic transmissionGlutamate receptor surface expressionPFC pyramidal neuronsYoung female ratsAdministration of estradiolLevels of aromatasePFC-dependent cognitionReceptor surface expressionBiosynthesis of estrogensEstrogen protectsPyramidal neuronsGlutamatergic deficitsRestraint stressFemale ratsEstrogen receptorGlutamate receptorsDetrimental effectsDimorphic effectsMemory impairmentStressed females
2012
Repeated Stress Causes Cognitive Impairment by Suppressing Glutamate Receptor Expression and Function in Prefrontal Cortex
Yuen EY, Wei J, Liu W, Zhong P, Li X, Yan Z. Repeated Stress Causes Cognitive Impairment by Suppressing Glutamate Receptor Expression and Function in Prefrontal Cortex. Neuron 2012, 73: 962-977. PMID: 22405206, PMCID: PMC3302010, DOI: 10.1016/j.neuron.2011.12.033.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerate6-Cyano-7-nitroquinoxaline-2,3-dioneAnalysis of VarianceAnimalsBicucullineCognition DisordersDisease Models, AnimalEndosomal Sorting Complexes Required for TransportExcitatory Amino Acid AntagonistsExcitatory Postsynaptic PotentialsF-Box ProteinsGABA-A Receptor AntagonistsImmunoprecipitationIn Vitro TechniquesMaleNedd4 Ubiquitin Protein LigasesNeuropsychological TestsPrefrontal CortexPyramidal CellsRatsRats, Sprague-DawleyReceptors, GlutamateRecognition, PsychologyRestraint, PhysicalRNA, Small InterferingStress, PsychologicalUbiquitin-Protein LigasesConceptsGlutamate receptor expressionPrefrontal cortexRepeated stressReceptor expressionRecognition memoryCognitive processesTemporal order recognition memoryPFC pyramidal neuronsStress-related mental disordersJuvenile male ratsGlutamatergic responsesGlutamatergic transmissionPyramidal neuronsMale ratsSynaptic transmissionStressed animalsInhibition of proteasomeMaladaptive changesGlucocorticoid receptorCognitive impairmentNR1 subunitMental disordersChronic stressUbiquitin/proteasome-mediated degradationReceptor turnover
2011
Amyloid β Peptide-(1–42) Induces Internalization and Degradation of β2 Adrenergic Receptors in Prefrontal Cortical Neurons*
Wang D, Yuen E, Zhou Y, Yan Z, Xiang Y. Amyloid β Peptide-(1–42) Induces Internalization and Degradation of β2 Adrenergic Receptors in Prefrontal Cortical Neurons*. Journal Of Biological Chemistry 2011, 286: 31852-31863. PMID: 21757762, PMCID: PMC3173113, DOI: 10.1074/jbc.m111.244335.Peer-Reviewed Original ResearchConceptsPrefrontal cortical neuronsCortical neuronsAdrenergic receptorsAMPA receptor-mediated miniature excitatory postsynaptic currentsG protein-coupled receptor kinase phosphorylationMiniature excitatory postsynaptic currentsCerebrum of miceΒAR agonist isoproterenolAmyloid precursor protein geneExcitatory postsynaptic currentsG protein-coupled receptor kinasesPrecursor protein geneProtein-coupled receptor kinasesReceptor subunit 1Mutant presenilin 1Receptor kinase phosphorylationGlutamatergic activityGlutamatergic regulationAβ bindsInduces desensitizationAβ treatmentPostsynaptic currentsΒ2-adrenergic receptorSynaptic activityAgonist isoproterenol
2010
Mechanisms for acute stress-induced enhancement of glutamatergic transmission and working memory
Yuen EY, Liu W, Karatsoreos IN, Ren Y, Feng J, McEwen BS, Yan Z. Mechanisms for acute stress-induced enhancement of glutamatergic transmission and working memory. Molecular Psychiatry 2010, 16: 156-170. PMID: 20458323, PMCID: PMC3108461, DOI: 10.1038/mp.2010.50.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiophysicsCells, CulturedCorticosteroneDose-Response Relationship, DrugDrug InteractionsElectric StimulationExcitatory Amino Acid AgentsExcitatory Postsynaptic PotentialsGene Expression RegulationGlutamic AcidGreen Fluorescent ProteinsHormone AntagonistsImmediate-Early ProteinsIn Vitro TechniquesLong-Term PotentiationMaleMaze LearningMemory, Short-TermMifepristonePatch-Clamp TechniquesPeptidesPrefrontal CortexProtein Serine-Threonine KinasesPyramidal CellsRab GTP-Binding ProteinsRatsRats, Sprague-DawleyReceptors, GlutamateRNA, Small InterferingStress, PsychologicalSynaptic TransmissionTime FactorsTransfectionConceptsPrefrontal cortexGlutamatergic transmissionAcid receptorsPFC pyramidal neuronsAspartic acid (NMDA) receptorsIsoxazolepropionic acid (AMPA) receptorsInduction of serumCorticosteroid stress hormonesFunction of NMDARsStress-induced enhancementSustained potentiationPyramidal neuronsCorticosterone treatmentSynaptic responsesSynaptic transmissionBehavioral stressorsGlucocorticoid-inducible kinaseGlucocorticoid receptorAcute stressStress hormonesΑ-aminoSurface expressionReceptorsUnderlying mechanismReceptor recycling
2009
Acute stress enhances glutamatergic transmission in prefrontal cortex and facilitates working memory
Yuen EY, Liu W, Karatsoreos IN, Feng J, McEwen BS, Yan Z. Acute stress enhances glutamatergic transmission in prefrontal cortex and facilitates working memory. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 14075-14079. PMID: 19666502, PMCID: PMC2729022, DOI: 10.1073/pnas.0906791106.Peer-Reviewed Original ResearchConceptsAcute stressPrefrontal cortexGlucocorticoid receptorPotentiation of NMDARPFC pyramidal neuronsLong-term potentiationKey brain regionsCorticosteroid stress hormonesGR-dependent mechanismGlutamatergic transmissionPyramidal neuronsSynaptic transmissionBehavioral stressorsSynaptic currentsPFC neuronsBehavioral testsBrain regionsChronic stressAMPAR subunitsRecurrent excitationStress hormonesBeneficial effectsCellular mechanismsSurface expressionNMDARDopamine D4 Receptors Regulate AMPA Receptor Trafficking and Glutamatergic Transmission in GABAergic Interneurons of Prefrontal Cortex
Yuen EY, Yan Z. Dopamine D4 Receptors Regulate AMPA Receptor Trafficking and Glutamatergic Transmission in GABAergic Interneurons of Prefrontal Cortex. Journal Of Neuroscience 2009, 29: 550-562. PMID: 19144855, PMCID: PMC2768380, DOI: 10.1523/jneurosci.5050-08.2009.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsCalcineurinColchicineDopamine AgentsEnzyme InhibitorsGamma-Aminobutyric AcidGlutamate DecarboxylaseGlutamic AcidGreen Fluorescent ProteinsIn Vitro TechniquesInterneuronsMiceMice, TransgenicMicrofilament ProteinsPatch-Clamp TechniquesPhalloidinePhosphoric Monoester HydrolasesPrefrontal CortexProtein TransportRatsReceptors, AMPAReceptors, Dopamine D4RNA, Small InterferingSynaptic TransmissionTransfectionTubulin ModulatorsConceptsGABAergic interneuronsAttention deficit hyperactivity disorderGABAergic inhibitionGlutamatergic transmissionPrefrontal cortexAMPAR-mediated synaptic transmissionLocal circuit neuronsPFC pyramidal neuronsExcitatory synaptic strengthAbnormal neural synchronyAMPA receptor traffickingDopamine D4 receptorAMPAR EPSCsDeficit hyperactivity disorderPFC interneuronsPyramidal neuronsMajor actin depolymerizing factorMemory disturbanceSynaptic transmissionAMPA receptorsCortical circuitsNeuronal activityPersistent suppressionInterneuronsMental disorders
2008
Activation of 5-HT2A/C Receptors Counteracts 5-HT1A Regulation of N-Methyl-D-aspartate Receptor Channels in Pyramidal Neurons of Prefrontal Cortex*
Yuen EY, Jiang Q, Chen P, Feng J, Yan Z. Activation of 5-HT2A/C Receptors Counteracts 5-HT1A Regulation of N-Methyl-D-aspartate Receptor Channels in Pyramidal Neurons of Prefrontal Cortex*. Journal Of Biological Chemistry 2008, 283: 17194-17204. PMID: 18442977, PMCID: PMC2427346, DOI: 10.1074/jbc.m801713200.Peer-Reviewed Original ResearchConceptsPFC pyramidal neuronsExtracellular signal-regulated kinasePyramidal neuronsPrefrontal cortexNMDAR currentsN-methyl-D-aspartate receptor currentsN-methyl-D-aspartate receptor channelsSynaptic NMDAR responsesNMDAR responsesNMDAR functionActivation of ERKDendritic processesReceptor currentsPFC neuronsSignal-regulated kinaseReceptor activationMental disordersImmunocytochemical studyIntact animalsCounteractive effectsReceptor channelsInhibitory effectNeuronsCellular knockdownDependent pathwayModulation of Neuronal Excitability by Serotonin-NMDA Interactions in Prefrontal Cortex
Zhong P, Yuen E, Yan Z. Modulation of Neuronal Excitability by Serotonin-NMDA Interactions in Prefrontal Cortex. Molecular And Cellular Neuroscience 2008, 38: 290-299. PMID: 18455431, PMCID: PMC2477738, DOI: 10.1016/j.mcn.2008.03.003.Peer-Reviewed Original ResearchMeSH Keywords8-Hydroxy-2-(di-n-propylamino)tetralinAction PotentialsAnimalsExcitatory Amino Acid AgonistsExtracellular Signal-Regulated MAP KinasesMAP Kinase Signaling SystemN-MethylaspartateOrgan Culture TechniquesPrefrontal CortexPyramidal CellsRatsRats, Sprague-DawleyReceptor, Serotonin, 5-HT1AReceptor, Serotonin, 5-HT2AReceptors, N-Methyl-D-AspartateSerotoninSerotonin Receptor AgonistsStress, PhysiologicalSwimmingConceptsExtracellular signal-regulated kinaseProtein kinase AProtein kinase CActivation of ERKSignal-regulated kinaseNeuronal excitabilityKinase AMolecule downstreamERK activationPrefrontal cortexKinase CNMDA receptorsStressful conditionsMental disordersBiochemical evidencePFC neuronal firingPFC pyramidal neuronsPresence of NMDAAction potential firingNMDA-dependent mannerDPAT effectsAgonist alphaPyramidal neuronsReceptor subtypesActivation
2006
Adrenergic modulation of NMDA receptors in prefrontal cortex is differentially regulated by RGS proteins and spinophilin
Liu W, Yuen EY, Allen PB, Feng J, Greengard P, Yan Z. Adrenergic modulation of NMDA receptors in prefrontal cortex is differentially regulated by RGS proteins and spinophilin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2006, 103: 18338-18343. PMID: 17101972, PMCID: PMC1838752, DOI: 10.1073/pnas.0604560103.Peer-Reviewed Original ResearchConceptsExcitatory postsynaptic currentsNMDA receptorsAlpha1-ARNMDAR currentsPrefrontal cortexNoradrenergic systemPFC neuronsAlpha2-AR agonistNMDAR EPSC amplitudePFC pyramidal neuronsPaired-pulse ratioAlpha2-adrenergic receptorsPhospholipase C-inositolEffect of RGS4Norepinephrine transporter inhibitorG protein-coupled receptorsSpinophilin knockout miceProtein-coupled receptorsAdrenergic modulationPyramidal neuronsPFC slicesPostsynaptic currentsMood controlMutant miceTransporter inhibitors
2005
Activation of Dopamine D4 Receptors Induces Synaptic Translocation of Ca2+/Calmodulin-Dependent Protein Kinase II in Cultured Prefrontal Cortical Neurons
Gu Z, Jiang Q, Yuen E, Yan Z. Activation of Dopamine D4 Receptors Induces Synaptic Translocation of Ca2+/Calmodulin-Dependent Protein Kinase II in Cultured Prefrontal Cortical Neurons. Molecular Pharmacology 2005, 69: 813-822. PMID: 16365279, DOI: 10.1124/mol.105.018853.Peer-Reviewed Original ResearchConceptsD4 receptor activationDependent protein kinase IIProtein kinase IIAlpha-CaMKIID4 receptorsDopamine D4 receptorCaMKII translocationKinase IIReceptor activationF-actinSynaptic translocationPostsynaptic sitesBinding of CaMKIITrisphosphate receptor/Ca2Receptor-mediated excitatory postsynaptic currentsAMPA receptor-mediated excitatory postsynaptic currentsPhospholipase C/inositolPrefrontal cortexCalmodulin binding siteExcitatory postsynaptic currentsGlutamate receptor 1 (GluR1) subunitPrefrontal cortical neuronsD4 receptor stimulationAutophosphorylation sitesSubcellular localizationSerotonin 5-HT1A Receptors Regulate NMDA Receptor Channels through a Microtubule-Dependent Mechanism
Yuen EY, Jiang Q, Chen P, Gu Z, Feng J, Yan Z. Serotonin 5-HT1A Receptors Regulate NMDA Receptor Channels through a Microtubule-Dependent Mechanism. Journal Of Neuroscience 2005, 25: 5488-5501. PMID: 15944377, PMCID: PMC6724987, DOI: 10.1523/jneurosci.1187-05.2005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesDendritesExtracellular Signal-Regulated MAP KinasesIn Vitro TechniquesKinesinsMembrane PotentialsMicrotubulesMolecular Motor ProteinsNeuronsPatch-Clamp TechniquesPrefrontal CortexProtein SubunitsProtein TransportRatsReceptor, Serotonin, 5-HT1AReceptors, N-Methyl-D-AspartateSerotoninSerotonin 5-HT1 Receptor AgonistsSynapsesConceptsMicrotubule-dependent mechanismMEK/ERKNMDA receptorsPrefrontal cortexMicrotubule stabilityDendritic transportNMDAR interactionMicrotubule assemblyBiochemical evidenceMEK inhibitorsPFC pyramidal neuronsNMDA receptor channelsRole of serotoninCaMKIIERKNeuronal dendritesNMDAR functionMicrotubule stabilizerPathological conditionsPrimary targetReceptor channelsPyramidal neuronsNMDAR currentsSerotonin systemReceptors