2015
Actin Mechanics and Fragmentation*
De La Cruz EM, Gardel ML. Actin Mechanics and Fragmentation*. Journal Of Biological Chemistry 2015, 290: 17137-17144. PMID: 25957404, PMCID: PMC4498053, DOI: 10.1074/jbc.r115.636472.Peer-Reviewed Original Research
2011
Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light Chain
Prochniewicz E, Pierre A, McCullough BR, Chin HF, Cao W, Saunders LP, Thomas DD, De La Cruz EM. Actin Filament Dynamics in the Actomyosin VI Complex Is Regulated Allosterically by Calcium–Calmodulin Light Chain. Journal Of Molecular Biology 2011, 413: 584-592. PMID: 21910998, PMCID: PMC3633491, DOI: 10.1016/j.jmb.2011.08.058.Peer-Reviewed Original ResearchConceptsActin filament dynamicsMyosin VIFilament dynamicsMicrosecond dynamicsCaM-dependent mannerCalmodulin light chainsLight chainActin bindingActin filamentsDependent CaMIQ domainCaM-dependent regulationFluorescence microscopyEnzymatic activityTransient phosphorescence anisotropyATP utilizationFinal anisotropyMicrosecond rotational dynamicsPhosphorescence anisotropyMyosinStructural dynamicsAnisotropy decaySuch modulationActinRegulation
2001
Kinetic Mechanism and Regulation of Myosin VI*
De La Cruz E, Ostap E, Sweeney H. Kinetic Mechanism and Regulation of Myosin VI*. Journal Of Biological Chemistry 2001, 276: 32373-32381. PMID: 11423557, DOI: 10.1074/jbc.m104136200.Peer-Reviewed Original ResearchConceptsHeavy chain phosphorylationMyosin VIPhysiological nucleotide concentrationsADP releaseHigh duty ratio motorMolecular basisUnique adaptationsActin filamentsATP bindsATPase cycleNative dimerRate-limiting stepDetailed kinetic analysisChain phosphorylationRegulationNucleotide concentrationsDiffusional encounterMyosinLow affinityMutantsProcessivityKinetic analysisPhosphorylationActinBinds