2020
Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches
Pandit NG, Cao W, Bibeau J, Johnson-Chavarria EM, Taylor EW, Pollard TD, De La Cruz EM. Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 13519-13528. PMID: 32461373, PMCID: PMC7306818, DOI: 10.1073/pnas.1911183117.Peer-Reviewed Original ResearchConceptsActin filament branchesArp2/3 complexMother filamentFilament branchesTotal internal reflection fluorescence microscopyEssential cellular functionsMechanical forcesActin filament networkReflection fluorescence microscopyCellular functionsActin networkCell motilityComplex generatesActin filamentsArp2/3Filament networkFluorescence microscopyState 1Branch junctionsState 2FilamentsComplexesPhosphate releaseMuscle actinADP
2009
Chapter 6 Kinetic and Equilibrium Analysis of the Myosin ATPase
De La Cruz EM, Ostap EM. Chapter 6 Kinetic and Equilibrium Analysis of the Myosin ATPase. Methods In Enzymology 2009, 455: 157-192. PMID: 19289206, PMCID: PMC2921708, DOI: 10.1016/s0076-6879(08)04206-7.Peer-Reviewed Original Research
2005
Vertebrate Myosin VIIb Is a High Duty Ratio Motor Adapted for Generating and Maintaining Tension*
Henn A, De La Cruz EM. Vertebrate Myosin VIIb Is a High Duty Ratio Motor Adapted for Generating and Maintaining Tension*. Journal Of Biological Chemistry 2005, 280: 39665-39676. PMID: 16186105, DOI: 10.1074/jbc.m507667200.Peer-Reviewed Original ResearchConceptsClass VII myosinMyosin VIIbActin filament concentrationUnique cellular functionsInner ear hair cellsCatalytic motor domainEar hair cellsActin filament bundlesNon-muscle myosinSteady-state ATPase activityActin cytoskeletonHigh conservationCellular functionsMyosin VIIa functionCDNA libraryActin bindingDeafness phenotypeMembrane surface receptorsATPase cycleEnzymatic propertiesSurface receptorsFilament bundlesIntestinal brush borderMolecular motorsQuantitative equilibrium
2004
Relating biochemistry and function in the myosin superfamily
De La Cruz EM, Ostap EM. Relating biochemistry and function in the myosin superfamily. Current Opinion In Cell Biology 2004, 16: 61-67. PMID: 15037306, DOI: 10.1016/j.ceb.2003.11.011.Peer-Reviewed Original Research