2013
Forward genetics defines Xylt1 as a key, conserved regulator of early chondrocyte maturation and skeletal length
Mis EK, Liem KF, Kong Y, Schwartz NB, Domowicz M, Weatherbee SD. Forward genetics defines Xylt1 as a key, conserved regulator of early chondrocyte maturation and skeletal length. Developmental Biology 2013, 385: 67-82. PMID: 24161523, PMCID: PMC3895954, DOI: 10.1016/j.ydbio.2013.10.014.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBone and BonesCell DifferentiationCell ProliferationChondrocytesDwarfismFibroblast Growth FactorsHedgehog ProteinsMiceMice, Inbred C57BLMice, TransgenicMutation, MissenseOsteogenesisParathyroid Hormone-Related ProteinPentosyltransferasesSequence Analysis, DNASignal TransductionConceptsChondrocyte maturationCartilage templateN-ethyl-N-nitrosourea (ENU) mutagenesis screenSkeletal precursor cellsWild-type embryosHigh-throughput sequencingHuman birth defectsProteoglycan core proteinMutagenesis screenSequence captureVertebrate bodySubcellular localizationProteoglycan functionCoordinated processMouse mutantsMutantsAbnormal bone developmentMissense mutationsCore proteinBone developmentSkeletal elementsPrecursor cellsDwarfismMaturationGAG chains
2012
High-throughput fluorescence-based isolation of live C. elegans larvae
Fernandez A, Bargmann B, Mis E, Edgley M, Birnbaum K, Piano F. High-throughput fluorescence-based isolation of live C. elegans larvae. Nature Protocols 2012, 7: 1502-1510. PMID: 22814389, PMCID: PMC5274720, DOI: 10.1038/nprot.2012.084.Peer-Reviewed Original ResearchConceptsGenetic interactionsChemical genetic screenNematode Caenorhabditis elegansFluorescence-activated cell sortingSelection of animalsTermination mutantsCaenorhabditis elegansSorting animalsGenetic screeningChemical screeningGFP expressionCell sortingLarval stageLive animalsHigh-speed sortingMutantsAnimalsFACSIsolatesGenotypesNematodesHomozygotes