1996
A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension
Segal A, Boulpaep E, Maunsbach A. A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension. American Journal Of Physiology 1996, 270: c1843-c1863. PMID: 8764170, DOI: 10.1152/ajpcell.1996.270.6.c1843.Peer-Reviewed Original ResearchConceptsEpithelial polarityFunctional polaritySingle cellsMembrane trafficking pathwaysCell surface polarityBasolateral membrane domainsIon channel proteinsBasolateral membraneApical brush borderCell polarityTrafficking pathwaysActin cytoskeletonMembrane domainsProximal tubule cellsMost epithelial cellsChannel proteinsZO-1 proteinTubule cellsJunctional complexesTight junctional complexesPolarized structureSingle cell modelEpithelial cellsRenal proximal tubule cellsZO-1
1993
Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells.
Korbmacher C, Segal A, Fejes-Tóth G, Giebisch G, Boulpaep E. Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells. The Journal Of General Physiology 1993, 102: 761-793. PMID: 8270912, PMCID: PMC2229168, DOI: 10.1085/jgp.102.4.761.Peer-Reviewed Original ResearchConceptsWhole-cell currentsWhole-cell conductanceConfluent cellsWhole-cell membrane currentsBasolateral K channelsCells 24 hK conductancePA/cellMicroM glibenclamideAmiloride-sensitive conductanceTransgenic miceReabsorb sodiumOutward currentsM amilorideGlibenclamide inhibitionDuct cellsPrincipal cellsMembrane currentsBath KLarge depolarizationChloride conductanceK channelsLipophilic drugsPermeable supportsMice
1990
Bicarbonate transport mechanisms in the Ambystoma kidney proximal tubule: transepithelial potential measurements.
Bock J, Boulpaep E. Bicarbonate transport mechanisms in the Ambystoma kidney proximal tubule: transepithelial potential measurements. The Yale Journal Of Biology And Medicine 1990, 63: 529-47. PMID: 2092412, PMCID: PMC2589409.Peer-Reviewed Original Research
1989
MECHANISMS OF WATER TRANSPORT BY EPITHELIAL CELLS
Tripathi S, Boulpaep E. MECHANISMS OF WATER TRANSPORT BY EPITHELIAL CELLS. Quarterly Journal Of Experimental Physiology And Cognate Medical Sciences 1989, 74: 385-417. PMID: 2678220, DOI: 10.1113/expphysiol.1989.sp003288.Peer-Reviewed Original Research
1987
Ultrastructural changes in isolated perfused proximal tubules during osmotic water flow
Maunsbach A, Tripathi S, Boulpaep E. Ultrastructural changes in isolated perfused proximal tubules during osmotic water flow. American Journal Of Physiology 1987, 253: f1091-f1104. PMID: 3425719, DOI: 10.1152/ajprenal.1987.253.6.f1091.Peer-Reviewed Original Research
1984
Role of aldosterone in the mechanism of potassium adaptation in the initial collecting tubule
Hirsch D, Kashgarian M, Boulpaep E, Hayslett J. Role of aldosterone in the mechanism of potassium adaptation in the initial collecting tubule. Kidney International 1984, 26: 798-807. PMID: 6099857, DOI: 10.1038/ki.1984.221.Peer-Reviewed Original ResearchConceptsInitial collecting tubuleCollecting tubuleDistal convolutionAldosterone levelsPotassium adaptationChronic potassium loadingMineralocorticoid-sensitive tissuesRole of aldosteronePlasma aldosterone levelsAction of aldosteroneIntact adrenal glandsPotassium loadingPhysiological plasma levelsChronic hyperaldosteronismTransepithelial potential differenceChronic administrationEpithelial changesAdrenal glandPlasma levelsDietary potassium loadingBasal valuesAldosteroneTubule cellsNephron segmentsTarget tissuesQuantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus
Maunsbach A, Boulpaep E. Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus. American Journal Of Physiology 1984, 246: f710-f724. PMID: 6720973, DOI: 10.1152/ajprenal.1984.246.5.f710.Peer-Reviewed Original ResearchMeSH KeywordsAmbystomaAnimalsCell MembraneEpitheliumFemaleKidney Tubules, ProximalMitochondriaNecturusConceptsBasal cell membraneCell membraneStructure-function correlationsIntercellular spacesBasal cell surfaceLateral cell membranesBasal extracellular labyrinthMorphometric comparative analysisVolume of cellsInterspecies variabilityCell surfaceAmbystomaQuantitative ultrastructureElaborate organizationBasolateral membraneLateral intercellular spacesSpeciesSalamander Ambystoma tigrinumExtracellular channelsMembraneUltrastructureAmbystoma tigrinumFunctional propertiesProximal tubules
1982
Cellular and paracellular resistances of theNecturus proximal tubule
Guggino W, Windhager E, Boulpaep E, Giebisch G. Cellular and paracellular resistances of theNecturus proximal tubule. The Journal Of Membrane Biology 1982, 67: 143-154. PMID: 7097759, DOI: 10.1007/bf01868657.Peer-Reviewed Original ResearchIonic conductive properties and electrophysiology of the rabbit cortical collecting tubule
O'Neil R, Boulpaep E. Ionic conductive properties and electrophysiology of the rabbit cortical collecting tubule. American Journal Of Physiology 1982, 243: f81-f95. PMID: 7091368, DOI: 10.1152/ajprenal.1982.243.1.f81.Peer-Reviewed Original Research
1981
Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport
Sackin H, Boulpaep E. Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport. American Journal Of Physiology 1981, 241: f540-f555. PMID: 7304748, DOI: 10.1152/ajprenal.1981.241.5.f540.Peer-Reviewed Original ResearchThe rheogenic nature of sodium transport in leaky epithelia studied by equivalent circuit analysis.
Sackin H, Boulpaep E. The rheogenic nature of sodium transport in leaky epithelia studied by equivalent circuit analysis. Progress In Clinical And Biological Research 1981, 73: 57-66. PMID: 6275400.Peer-Reviewed Original Research
1979
Modulation of cell membrane area in renal collecting tubules by corticosteroid hormones.
Wade J, O'Neil R, Pryor J, Boulpaep E. Modulation of cell membrane area in renal collecting tubules by corticosteroid hormones. Journal Of Cell Biology 1979, 81: 439-445. PMID: 468913, PMCID: PMC2110313, DOI: 10.1083/jcb.81.2.439.Peer-Reviewed Original ResearchEquivalent electrical circuit analysis and rheogenic pumps in epithelia.
Boulpaep E, Sackin H. Equivalent electrical circuit analysis and rheogenic pumps in epithelia. The FASEB Journal 1979, 38: 2030-6. PMID: 437145.Peer-Reviewed Original Research
1978
Factors influencing transepithelial potential difference in mammalian distal tubule
Hayslett J, Boulpaep E, Giebisch G. Factors influencing transepithelial potential difference in mammalian distal tubule. American Journal Of Physiology 1978, 234: f182-f191. PMID: 629352, DOI: 10.1152/ajprenal.1978.234.3.f182.Peer-Reviewed Original Research
1977
Role of the paracellular pathway in isotonic fluid movement across the renal tubule.
Boulpaep E, Sackin H. Role of the paracellular pathway in isotonic fluid movement across the renal tubule. The Yale Journal Of Biology And Medicine 1977, 50: 115-31. PMID: 331692, PMCID: PMC2595392.Peer-Reviewed Original Research
1976
Electrical phenomena in the nephron
Boulpaep E. Electrical phenomena in the nephron. Kidney International 1976, 9: 88-102. PMID: 940266, DOI: 10.1038/ki.1976.14.Peer-Reviewed Original ResearchConceptsSingle barrierAvailable experimental dataIon movementElectrical conductanceElectrical phenomenaSingle cell membraneSingle membrane levelElectric currentElectrical propertiesPotential differenceExperimental dataDiffusion barrierDistinctive physical propertiesElectrical characteristicsPhysical propertiesIndividual cell membranesDiffusion boundariesElectrical behaviorPotential gradientMembrane approachConjugate forcesBiological membranesElectrochemical potential gradientTransport processesMicroscopic level
1971
Electrolyte transport in kidney tubule cells
Giebisch G, Boulpaep E, Whittembury G. Electrolyte transport in kidney tubule cells. Philosophical Transactions Of The Royal Society B Biological Sciences 1971, 262: 175-196. PMID: 4399217, DOI: 10.1098/rstb.1971.0088.Peer-Reviewed Original ResearchConceptsTubule cellsTubular levelKidney tubule cellsNet Na transportActive K uptakePotassium secretionNa-K exchange pumpK secretionProximal tubular levelLuminal cell membraneElectrolyte transportNa pumpLines of evidenceTranstubular movementNa transportNet NaCell compartmentExchange pumpSecretionInterstitial fluidCellsLumenPassive leakIonic pumpsUptake