2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
1993
Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells.
Korbmacher C, Segal A, Fejes-Tóth G, Giebisch G, Boulpaep E. Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells. The Journal Of General Physiology 1993, 102: 761-793. PMID: 8270912, PMCID: PMC2229168, DOI: 10.1085/jgp.102.4.761.Peer-Reviewed Original ResearchConceptsWhole-cell currentsWhole-cell conductanceConfluent cellsWhole-cell membrane currentsBasolateral K channelsCells 24 hK conductancePA/cellMicroM glibenclamideAmiloride-sensitive conductanceTransgenic miceReabsorb sodiumOutward currentsM amilorideGlibenclamide inhibitionDuct cellsPrincipal cellsMembrane currentsBath KLarge depolarizationChloride conductanceK channelsLipophilic drugsPermeable supportsMice