2019
Human Physiology
Boulpaep E, Boron W. Human Physiology. 2019 DOI: 10.1016/b978-0-12-801238-3.62186-4.Peer-Reviewed Original Research
2003
Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells
Basavappa S, Mangel A, Boulpaep E. Calcium-dependent, swelling-activated K+ conductance in human neuroblastoma cells. Biochemical And Biophysical Research Communications 2003, 308: 759-763. PMID: 12927783, DOI: 10.1016/s0006-291x(03)01481-5.Peer-Reviewed Original ResearchConceptsHuman neuroblastoma cell line CHP-100CHP-100Regulatory volume decreaseCell-attached patch-clamp studiesFree extracellular solutionHuman neuroblastoma cellsSwelling-activated ClPatch-clamp studiesFura-2Extracellular osmolarityFree extracellularNeuroblastoma cellsMOsm/Hypoosmotic solutionCell swellingExtracellular solutionCl- channelsHypoosmotic stressPresent studyMost mammalian cellsEGTACellsVolume decreaseEffluxPrevious studies
2000
An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel
Choi I, Aalkjaer C, Boulpaep E, Boron W. An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel. Nature 2000, 405: 571-575. PMID: 10850716, DOI: 10.1038/35014615.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic AcidAnimalsBicarbonatesBiological TransportCarrier ProteinsChloridesCloning, OrganismDNA, ComplementaryElectrophysiologyHumansMolecular Sequence DataRatsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, ComplementarySequence Homology, Amino AcidSodiumSodium ChannelsSodium-Bicarbonate SymportersXenopus
1993
Endothelin increases [Ca2+]i in M-1 mouse cortical collecting duct cells by a dual mechanism
Korbmacher C, Boulpaep E, Giebisch G, Geibel J. Endothelin increases [Ca2+]i in M-1 mouse cortical collecting duct cells by a dual mechanism. American Journal Of Physiology 1993, 265: c349-c357. PMID: 8368264, DOI: 10.1152/ajpcell.1993.265.2.c349.Peer-Reviewed Original ResearchConceptsEndothelin-1Intracellular storesDuct cellsNifedipine-sensitive Ca2Intracellular calcium concentrationParticipation of Ca2Endothelin increaseFura-2Extracellular calciumExtracellular Ca2Arginine vasopressinIntracellular Ca2Calcium concentrationInitial Ca2Fluorescent imaging systemInitial exposurePlateau phaseMiceEntry mechanismDual mechanismCa2Second Ca2CellsNifedipineVasopressin
1992
Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel.
Ahmad I, Korbmacher C, Segal A, Cheung P, Boulpaep E, Barnstable C. Mouse cortical collecting duct cells show nonselective cation channel activity and express a gene related to the cGMP-gated rod photoreceptor channel. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 10262-10266. PMID: 1279673, PMCID: PMC50318, DOI: 10.1073/pnas.89.21.10262.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, NorthernCell LineCyclic GMPDNAIon Channel GatingIon ChannelsKidney CortexKidney Tubules, CollectingMembrane PotentialsMiceMolecular Sequence DataOligodeoxyribonucleotidesPhotoreceptor CellsPoly APolymerase Chain ReactionRatsRNARNA, MessengerSequence Homology, Nucleic AcidConceptsNonselective cation channelsPhotoreceptor channelDuct cellsCation channel probeCation channelsChannel activityPolymerase chain reactionNonselective cation channel activityCGMP decreaseCation channel activityChannel subunitsCytoplasmic applicationChain reactionRod photoreceptorsNorthern blot analysisRetinal cGMPBlot analysisCGMPRat kidney cDNA libraryChannel genesMiceSingle-channel conductanceOpen probabilityCellsCalcium removalDifferentiated Transport Properties of Primary Cultured Distal Nephron
White S, Boulpaep E, Giebisch G. Differentiated Transport Properties of Primary Cultured Distal Nephron. Cellular Physiology And Biochemistry 1992, 2: 323-335. DOI: 10.1159/000154655.Peer-Reviewed Original ResearchAcid secretionNet acid secretionEquivalent short-circuit currentIncubation of monolayersRabbit kidney cortexCollagen-coated permeable supportsDistal nephronPositive cellsKidney cortexPercoll density centrifugationNephron fragmentsBasolateral fluidInsensitive componentsSecretionTransport of sodiumBasolateral solutionPermeable supportsH incubationDensity centrifugationCellsAldosteroneCulture mediumCortexRich cellsNephron
1983
Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. The Journal Of General Physiology 1983, 81: 29-52. PMID: 6833996, PMCID: PMC2215563, DOI: 10.1085/jgp.81.1.29.Peer-Reviewed Original ResearchConceptsProximal tubulesProximal tubule cellsRenal proximal tubulesTiger salamander Ambystoma tigrinumPH-sensitive microelectrodesFree RingerMaximal effectTubule cellsTransient riseDiuretic amilorideBasolateral sideBasolateral solutionExponential time courseSalamander Ambystoma tigrinumTime courseCl removalMaximal rateBasolateral membraneNormal rateTubulesAmbystoma tigrinumPH regulationCellsActive transportRecovery
1971
Electrolyte transport in kidney tubule cells
Giebisch G, Boulpaep E, Whittembury G. Electrolyte transport in kidney tubule cells. Philosophical Transactions Of The Royal Society B Biological Sciences 1971, 262: 175-196. PMID: 4399217, DOI: 10.1098/rstb.1971.0088.Peer-Reviewed Original ResearchConceptsTubule cellsTubular levelKidney tubule cellsNet Na transportActive K uptakePotassium secretionNa-K exchange pumpK secretionProximal tubular levelLuminal cell membraneElectrolyte transportNa pumpLines of evidenceTranstubular movementNa transportNet NaCell compartmentExchange pumpSecretionInterstitial fluidCellsLumenPassive leakIonic pumpsUptake