2010
Substrate specificity of Rhbg: ammonium and methyl ammonium transport
Nakhoul N, Abdulnour-Nakhoul S, Boulpaep E, Rabon E, Schmidt E, Hamm L. Substrate specificity of Rhbg: ammonium and methyl ammonium transport. American Journal Of Physiology - Cell Physiology 2010, 299: c695-c705. PMID: 20592240, PMCID: PMC2944323, DOI: 10.1152/ajpcell.00019.2010.Peer-Reviewed Original Research
2004
Characteristics of renal Rhbg as an NH4+ transporter
Nakhoul N, DeJong H, Abdulnour-Nakhoul S, Boulpaep E, Hering-Smith K, Hamm L. Characteristics of renal Rhbg as an NH4+ transporter. American Journal Of Physiology. Renal Physiology 2004, 288: f170-f181. PMID: 15353405, DOI: 10.1152/ajprenal.00419.2003.Peer-Reviewed Original Research
1998
Transcellular Chloride Pathways in Ambystoma Proximal Tubule
Abdulnour-Nakhoul S, Boulpaep E. Transcellular Chloride Pathways in Ambystoma Proximal Tubule. The Journal Of Membrane Biology 1998, 166: 15-35. PMID: 9784583, DOI: 10.1007/s002329900444.Peer-Reviewed Original ResearchConceptsProximal tubulesChloride channel blockerContribution of transportersAmbystoma proximal tubuleTransepithelial potential differenceDisulfonic stilbeneCl-HCO3 exchangeFree RingerLines of evidenceTubulesBasolateral cell membraneLumenNa-independent ClMembrane potentialBasolateral membraneApical membrane potentialAiClBlockersProperties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Properties of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 139-160. PMID: 9417141, PMCID: PMC1887768, DOI: 10.1085/jgp.111.1.139.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBariumBiological TransportCationsCells, CulturedCytidine TriphosphateDiazoxideDiureticsElectric ConductivityGlyburideGuanosine TriphosphateHypoglycemic AgentsInosine TriphosphateIon Channel GatingKidney Tubules, ProximalKineticsMicrovilliPatch-Clamp TechniquesPotassium ChannelsSodium Chloride Symporter InhibitorsSodium-Potassium-Exchanging ATPaseThalliumThymine NucleotidesUridine TriphosphateConceptsProximal tubule cellsProximal tubulesTubule cellsBasolateral membraneChannel opener diazoxideChannel activityPump-leak couplingRenal proximal tubulesPivotal physiological rolesOpener diazoxideMillimolar dosesMM ATPHigh dosesPotassium conductanceCell membrane potentialInternal Mg2Cytosolic additionATPase pumpRegulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule
Mauerer U, Boulpaep E, Segal A. Regulation of an Inwardly Rectifying ATP-sensitive K+ Channel in the Basolateral Membrane of Renal Proximal Tubule. The Journal Of General Physiology 1998, 111: 161-180. PMID: 9417142, PMCID: PMC1887764, DOI: 10.1085/jgp.111.1.161.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmbystomaAnimalsBiological TransportCalciumCell MembraneColforsinCyclic AMP-Dependent Protein KinasesEnzyme ActivationEpithelial CellsHydrogen-Ion ConcentrationIon Channel GatingKidney Tubules, ProximalPatch-Clamp TechniquesPhosphorylationPotassium ChannelsProtein Kinase CSodium-Potassium-Exchanging ATPaseConceptsBasolateral membraneProtein kinase AActin cytoskeletonATPase pumpOuter membraneProtein kinaseKinase ACellular metabolismKATP channelsSecond messengerStimulation of transportTransport activityPhysiological importanceMembrane stretchPhorbol esterChannel activityMembrane patchesRegulationIntracellularO patchesFunctional couplingHypotonic swellingCell-attached patchesCell depolarizationRenal proximal tubules
1997
Tubule electrophysiology: from single channels back to the renal epithelium.
Boulpaep E. Tubule electrophysiology: from single channels back to the renal epithelium. Wiener Klinische Wochenschrift 1997, 109: 489-92. PMID: 9261991.Peer-Reviewed Original ResearchConceptsMacroscopic membrane propertiesMicroscopic parametersSingle channel proteinsElectrical propertiesIon flowMacroscopic referenceSingle barrierMembrane patchesRenal epitheliumSingle channelRenal ion transportRenal ion channelsRegulatory domainExperimental errorChannel proteinsPore conductivityIon channelsWhole-cell conductancePatch-clamp technologyBasolateral membraneRenal tubule cellsCell membrane parametersIon selectivityIon transportOpen probabilityThe electrogenic Na/HCO3 cotransporter.
Boron W, Fong P, Hediger M, Boulpaep E, Romero M. The electrogenic Na/HCO3 cotransporter. Wiener Klinische Wochenschrift 1997, 109: 445-56. PMID: 9261985.Peer-Reviewed Original ResearchConceptsElectrogenic Na/HCO3 cotransporterProximal tubulesHCO3- cotransporterRenal proximal tubulesCO2/10 mM HCO3HCO3- transportersRegulation of intracellularBasolateral membraneNumber of tissuesNative oocytesRapid hyperpolarizationXenopus laevis oocytesExtracellular ClBicarbonate cotransporterOocyte expressionCotransporterMM HCO3OocytesCell typesMost cellsMembrane potentialAmino acid homologySingle cloneWater-injected controlsTubules
1996
A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension
Segal A, Boulpaep E, Maunsbach A. A novel preparation of dissociated renal proximal tubule cells that maintain epithelial polarity in suspension. American Journal Of Physiology 1996, 270: c1843-c1863. PMID: 8764170, DOI: 10.1152/ajpcell.1996.270.6.c1843.Peer-Reviewed Original ResearchConceptsEpithelial polarityFunctional polaritySingle cellsMembrane trafficking pathwaysCell surface polarityBasolateral membrane domainsIon channel proteinsBasolateral membraneApical brush borderCell polarityTrafficking pathwaysActin cytoskeletonMembrane domainsProximal tubule cellsMost epithelial cellsChannel proteinsZO-1 proteinTubule cellsJunctional complexesTight junctional complexesPolarized structureSingle cell modelEpithelial cellsRenal proximal tubule cellsZO-1
1984
Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus
Maunsbach A, Boulpaep E. Quantitative ultrastructure and functional correlates in proximal tubule of Ambystoma and Necturus. American Journal Of Physiology 1984, 246: f710-f724. PMID: 6720973, DOI: 10.1152/ajprenal.1984.246.5.f710.Peer-Reviewed Original ResearchConceptsBasal cell membraneCell membraneStructure-function correlationsIntercellular spacesBasal cell surfaceLateral cell membranesBasal extracellular labyrinthMorphometric comparative analysisVolume of cellsInterspecies variabilityCell surfaceAmbystomaQuantitative ultrastructureElaborate organizationBasolateral membraneLateral intercellular spacesSpeciesSalamander Ambystoma tigrinumExtracellular channelsMembraneUltrastructureAmbystoma tigrinumFunctional propertiesProximal tubules
1983
Rheogenic transport in the renal proximal tubule.
Sackin H, Boulpaep E. Rheogenic transport in the renal proximal tubule. The Journal Of General Physiology 1983, 82: 819-851. PMID: 6319539, PMCID: PMC2228722, DOI: 10.1085/jgp.82.6.819.Peer-Reviewed Original ResearchIntracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Basolateral HCO3- transport. The Journal Of General Physiology 1983, 81: 53-94. PMID: 6833997, PMCID: PMC2215562, DOI: 10.1085/jgp.81.1.53.Peer-Reviewed Original ResearchConceptsProximal tubulesRenal proximal tubulesRapid fallBasolateral HCO3- transportBasolateral membraneTiger salamander Ambystoma tigrinumReversible fallNominal absencePresence of HCO3Removal of ClTransient depolarizationSimilar reductionBasolateral membrane potentialSalamander Ambystoma tigrinumHCO3- transportHCO3- transportersMembrane potentialOnly minor effectsAforementioned changesTubulesCl-sensitive microelectrodesDepolarizationConstant PCO2Opposite effectAmbystoma tigrinumIntracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange.
Boron W, Boulpaep E. Intracellular pH regulation in the renal proximal tubule of the salamander. Na-H exchange. The Journal Of General Physiology 1983, 81: 29-52. PMID: 6833996, PMCID: PMC2215563, DOI: 10.1085/jgp.81.1.29.Peer-Reviewed Original ResearchConceptsProximal tubulesProximal tubule cellsRenal proximal tubulesTiger salamander Ambystoma tigrinumPH-sensitive microelectrodesFree RingerMaximal effectTubule cellsTransient riseDiuretic amilorideBasolateral sideBasolateral solutionExponential time courseSalamander Ambystoma tigrinumTime courseCl removalMaximal rateBasolateral membraneNormal rateTubulesAmbystoma tigrinumPH regulationCellsActive transportRecovery
1981
Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport
Sackin H, Boulpaep E. Isolated perfused salamander proximal tubule. II. Monovalent ion replacement and rheogenic transport. American Journal Of Physiology 1981, 241: f540-f555. PMID: 7304748, DOI: 10.1152/ajprenal.1981.241.5.f540.Peer-Reviewed Original ResearchConceptsProximal tubulesEarly proximal tubuleTransepithelial resistanceSalamander proximal tubuleRenal epitheliumLumenBasolateral membrane