2023
194 Investigating the role of bromodomain-containing 8 isoforms in the innate immune response of human airway epithelial cells
Browne J, Bruscia E, Garrison A, Harris A, Egan M. 194 Investigating the role of bromodomain-containing 8 isoforms in the innate immune response of human airway epithelial cells. Journal Of Cystic Fibrosis 2023, 22: s101. DOI: 10.1016/s1569-1993(23)01124-4.Peer-Reviewed Original ResearchHuman airway epithelial cellsAirway epithelial cellsInnate immune responseImmune responseEpithelial cells
2012
Nebulized Hyaluronan Ameliorates lung inflammation in cystic fibrosis mice
Gavina M, Luciani A, Villella VR, Esposito S, Ferrari E, Bressani I, Casale A, Bruscia EM, Maiuri L, Raia V. Nebulized Hyaluronan Ameliorates lung inflammation in cystic fibrosis mice. Pediatric Pulmonology 2012, 48: 761-771. PMID: 22825912, DOI: 10.1002/ppul.22637.Peer-Reviewed Original ResearchConceptsLung inflammationCystic fibrosisLung tissueReactive oxygen speciesScnn1b-Tg miceHuman airway epithelial cellsSaline-treated miceChronic lung inflammationInflammatory protein-2Chronic respiratory diseasesPeroxisome Proliferator-Activated Receptor GammaPotential anti-inflammatory drugsAnti-inflammatory drugsAirway epithelial cellsCystic fibrosis miceIB3-1Myeloperoxidase levelsMIP-2MPO activityMacrophage infiltrationFibrosis miceTumor necrosisExogenous administrationTNFα expressionCF airways
2002
Isolation of CF cell lines corrected at ΔF508-CFTR locus by SFHR-mediated targeting
Bruscia E, Sangiuolo F, Sinibaldi P, Goncz KK, Novelli G, Gruenert DC. Isolation of CF cell lines corrected at ΔF508-CFTR locus by SFHR-mediated targeting. Gene Therapy 2002, 9: 683-685. PMID: 12032687, DOI: 10.1038/sj.gt.3301741.Peer-Reviewed Original ResearchConceptsSmall fragment homologous replacementAllele-specific PCRPrimer extension productsCell linesCFTR mRNAHuman airway epithelial cellsAllele-specific PCR amplificationΔF508 mutationCF transmembrane conductance regulator (CFTR) proteinExon 10CF cell lineMutant cell linesPCR amplificationAirway epithelial cellsEpithelial cellsGenomic DNA fingerprintingEndogenous CFTRHomologous replacementRegulator proteinDNA fingerprint analysisGenomic DNAWT mRNADNA fragmentsΔF508 alleleSequence analysis