2013
Targeting the Intracellular Environment in Cystic Fibrosis: Restoring Autophagy as a Novel Strategy to Circumvent the CFTR Defect
Villella VR, Esposito S, Bruscia EM, Maiuri MC, Raia V, Kroemer G, Maiuri L. Targeting the Intracellular Environment in Cystic Fibrosis: Restoring Autophagy as a Novel Strategy to Circumvent the CFTR Defect. Frontiers In Pharmacology 2013, 4: 1. PMID: 23346057, PMCID: PMC3549520, DOI: 10.3389/fphar.2013.00001.Peer-Reviewed Original ResearchCF transmembrane conductance regulatorIntracellular environmentMutant CF transmembrane conductance regulatorF508del-CFTR proteinBeclin-1CFTR channel activityTransmembrane conductance regulatorDefective CF transmembrane conductance regulatorDirect gene transferGeneral proteostasisProteostasis regulatorsConformational diseasesDisabled autophagyCFTR mutantsAutophagosome formationPlasma membraneConductance regulatorF508del-CFTRCommon deletion mutationsDeletion mutationsRecent pre-clinical evidenceIntracellular retentionGene transferCFTR defectProteostasis
1999
Gene transfection efficiency of tracheal epithelial cells by DC-Chol–DOPE/DNA complexes
Colosimo A, Serafino A, Sangiuolo F, Di Sario S, Bruscia E, Amicucci P, Novelli G, Dallapiccola B, Mossa G. Gene transfection efficiency of tracheal epithelial cells by DC-Chol–DOPE/DNA complexes. Biochimica Et Biophysica Acta 1999, 1419: 186-194. PMID: 10407070, DOI: 10.1016/s0005-2736(99)00067-x.Peer-Reviewed Original ResearchConceptsTransmission electron microscopyTransfection efficiencyDC-CholConfocal laser scanning microscopyCationic liposome/plasmid DNA complexesDNA complexesGene transfection efficiencyVivo gene therapyVitro gene transferPlasmid DNA complexesGene therapyDOPE liposomesElectron microscopyLiposome formulationsTracheal cellsGene transferLaser scanning microscopyCell linesMicroscopyTracheal epithelial cellsEpithelial cellsDramatic correlationScanning microscopyEfficiencyComplexes