2002
In vitrocorrection of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique
Sangiuolo F, Bruscia E, Serafino A, Nardone AM, Bonifazi E, Lais M, Gruenert DC, Novelli G. In vitrocorrection of cystic fibrosis epithelial cell lines by small fragment homologous replacement (SFHR) technique. BMC Medical Genomics 2002, 3: 8. PMID: 12243649, PMCID: PMC130050, DOI: 10.1186/1471-2350-3-8.Peer-Reviewed Original ResearchCell linesMembrane fusion pathwayCystic fibrosis epithelial cell lineDNA ratioSequence-based analysisCultured cell linesLipid/DNA ratioNormal airway epithelial cellsHomologous replacementMammalian cellsEndocytic pathwayLipid/DNA complexesEpithelial cell lineGene targetingFusion pathwayCellular factorsGenetic targetsCellular DNASmall fragmentsSpecific cell linesDNA complexesCellular processingSpecific mutationsDNASFHR
1999
Gene transfection efficiency of tracheal epithelial cells by DC-Chol–DOPE/DNA complexes
Colosimo A, Serafino A, Sangiuolo F, Di Sario S, Bruscia E, Amicucci P, Novelli G, Dallapiccola B, Mossa G. Gene transfection efficiency of tracheal epithelial cells by DC-Chol–DOPE/DNA complexes. Biochimica Et Biophysica Acta 1999, 1419: 186-194. PMID: 10407070, DOI: 10.1016/s0005-2736(99)00067-x.Peer-Reviewed Original ResearchConceptsTransmission electron microscopyTransfection efficiencyDC-CholConfocal laser scanning microscopyCationic liposome/plasmid DNA complexesDNA complexesGene transfection efficiencyVivo gene therapyVitro gene transferPlasmid DNA complexesGene therapyDOPE liposomesElectron microscopyLiposome formulationsTracheal cellsGene transferLaser scanning microscopyCell linesMicroscopyTracheal epithelial cellsEpithelial cellsDramatic correlationScanning microscopyEfficiencyComplexes