2021
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
Kroll F, Powell GT, Ghosh M, Gestri G, Antinucci P, Hearn TJ, Tunbak H, Lim S, Dennis HW, Fernandez JM, Whitmore D, Dreosti E, Wilson SW, Hoffman EJ, Rihel J. A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes. ELife 2021, 10: e59683. PMID: 33416493, PMCID: PMC7793621, DOI: 10.7554/elife.59683.Peer-Reviewed Original ResearchConceptsKnockout methodComplex mutant phenotypesLarge genetic screenRapid genetic screeningGenetic screenMutant phenotypeEffective CRISPRBiallelic knockoutMolecular rhythmsHuman genesInjected embryosCircadian clockLarval zebrafishBehavioral phenotypesMultiple genesComplex phenotypesCurrent CRISPRTriple knockoutAttractive modelGenesGenetic contributionZebrafishPhenotypeKnockoutCRISPR
2016
Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2
Hoffman EJ, Turner KJ, Fernandez JM, Cifuentes D, Ghosh M, Ijaz S, Jain RA, Kubo F, Bill BR, Baier H, Granato M, Barresi MJ, Wilson SW, Rihel J, State MW, Giraldez AJ. Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2. Neuron 2016, 89: 725-733. PMID: 26833134, PMCID: PMC4766582, DOI: 10.1016/j.neuron.2015.12.039.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAutistic DisorderDisease Models, AnimalEstrogensGene Expression RegulationGenisteinGreen Fluorescent ProteinsHumansLarvaLuminescent ProteinsMembrane ProteinsMotor ActivityMutationNerve Tissue ProteinsPhenotypePhytoestrogensPsychotropic DrugsSeizuresSleep-Wake Transition DisordersVesicular Glutamate Transport Protein 2Zebrafish