2006
MyD88 Deficiency Enhances Acquisition and Transmission of Borrelia burgdorferi by Ixodes scapularis Ticks
Bockenstedt LK, Liu N, Schwartz I, Fish D. MyD88 Deficiency Enhances Acquisition and Transmission of Borrelia burgdorferi by Ixodes scapularis Ticks. Infection And Immunity 2006, 74: 2154-2160. PMID: 16552045, PMCID: PMC1418887, DOI: 10.1128/iai.74.4.2154-2160.2006.Peer-Reviewed Original ResearchConceptsMyD88-/- miceToll-like receptorsWT miceB. burgdorferiB. burgdorferi strainsInnate immune cellsBurgdorferi strainsSkin inoculation siteB. burgdorferi DNAHigh virulence strainDegree of infectivityIxodes scapularis ticksMyD88 deficiencyMolecule MyD88Immune cellsMore spirochetesTransmission of BorreliaEffector functionsHost immunityBorrelia burgdorferi strainsClinical isolatesPathogen burdenMiceBlood meal hostsScapularis ticks
2003
Real-Time PCR for Simultaneous Detection and Quantification of Borrelia burgdorferi in Field-Collected Ixodes scapularis Ticks from the Northeastern United States
Wang G, Liveris D, Brei B, Wu H, Falco R, Fish D, Schwartz I. Real-Time PCR for Simultaneous Detection and Quantification of Borrelia burgdorferi in Field-Collected Ixodes scapularis Ticks from the Northeastern United States. Applied And Environmental Microbiology 2003, 69: 4561-4565. PMID: 12902243, PMCID: PMC169074, DOI: 10.1128/aem.69.8.4561-4565.2003.Peer-Reviewed Original Research
1997
Inhibition of efficient polymerase chain reaction amplification of Borrelia burgdorferi DNA in blood-fed ticks.
Schwartz I, Varde S, Nadelman R, Wormser G, Fish D. Inhibition of efficient polymerase chain reaction amplification of Borrelia burgdorferi DNA in blood-fed ticks. American Journal Of Tropical Medicine And Hygiene 1997, 56: 339-42. PMID: 9129540, DOI: 10.4269/ajtmh.1997.56.339.Peer-Reviewed Original ResearchConceptsB. burgdorferi DNABorrelia burgdorferi DNAB. burgdorferiInfection rateB. burgdorferi infectionPolymerase chain reaction analysisUtility of PCRInhibitory substancesBurgdorferi infectionChain reaction analysisPCR analysisPositive PCR signalUnfed ticksPolymerase chain reaction amplificationBlood-feeding ticksChain reaction amplificationTick lysatesEngorged ticksPCR amplificationBlood mealSuch inhibitionBurgdorferiEquivalent cohortsReaction amplificationInhibitors