2007
Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells
Solbrig CM, Saucier-Sawyer JK, Cody V, Saltzman WM, Hanlon DJ. Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells. Molecular Pharmaceutics 2007, 4: 47-57. PMID: 17217312, DOI: 10.1021/mp060107e.Peer-Reviewed Original ResearchConceptsPolymer nanoparticlesMixture of proteinsMolecular weight polymersRate of nanoparticlesProtein loadingEfficiency of encapsulationWeight polymersDissolved nanoparticlesNanoparticle formulationDifferent particle loadingsRelease propertiesNanoparticlesPreparation methodEncapsulation efficiencyComplex mixturesMolecular weightSDS-acrylamide gel electrophoresisSolvent extractionProtein assaysAgEncapsulationParticle loadingMixtureSpectrum of proteinsPromising approach
2005
Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles
Shen H, Ackerman AL, Cody V, Giodini A, Hinson ER, Cresswell P, Edelson RL, Saltzman WM, Hanlon DJ. Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles. Immunology 2005, 117: 78-88. PMID: 16423043, PMCID: PMC1782199, DOI: 10.1111/j.1365-2567.2005.02268.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationBiocompatible MaterialsBiodegradation, EnvironmentalB-LymphocytesCell LineCross-PrimingDendritic CellsEndosomesHistocompatibility Antigens Class IIHumansLactic AcidLymphocyte ActivationMiceMice, Inbred C57BLNanostructuresOvalbuminPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersSerum Albumin, BovineT-LymphocytesConceptsBone marrow-derived dendritic cellsMHC class I presentationAntigen-presenting cellsClass I presentationMHC class IExogenous antigensDendritic cellsClass IAntigen deliveryPrimary mouse bone marrow-derived dendritic cellsSoluble antigenMouse bone marrow-derived dendritic cellsMarrow-derived dendritic cellsProfessional antigen-presenting cellsMajor histocompatibility complex class IProtein-based vaccinationT cell responsesClassic MHC class IExogenous antigen presentationHistocompatibility complex class IAntigen-coated latex beadsCell-associated antigensInterleukin-2 secretionComplex class IEfficiency of presentation