1993
Identification of a 100‐kDa protein associated with nuclear ribonuclease P activity in Schizosaccharomyces pombe
ZIMMERLY S, DRAINAS D, SYLVERS L, Dieter S. Identification of a 100‐kDa protein associated with nuclear ribonuclease P activity in Schizosaccharomyces pombe. The FEBS Journal 1993, 217: 501-507. PMID: 8223594, DOI: 10.1111/j.1432-1033.1993.tb18270.x.Peer-Reviewed Original ResearchConceptsFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeGlycerol gradient fractionationCross-linking experimentsPrecursor tRNAsSchizosaccharomyces pombeRibonuclease PProtein interactsRNA componentProtein componentsP activityRibonuclease P activityApparent homogeneityDEAE-cellulose chromatographyPhosphocellulose chromatographySpecific fashionProtein
1990
Purification of the glutamyl-tRNA reductase from Chlamydomonas reinhardtii involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis.
Chen M, Jahn D, O'Neill G, Söll D. Purification of the glutamyl-tRNA reductase from Chlamydomonas reinhardtii involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis. Journal Of Biological Chemistry 1990, 265: 4058-4063. PMID: 2303495, DOI: 10.1016/s0021-9258(19)39702-9.Peer-Reviewed Original ResearchConceptsGlu-tRNA reductaseGlutamyl-tRNA reductaseGlu-tRNAChlamydomonas reinhardtiiTRNA-dependent transformationChloroplasts of plantsDelta-aminolevulinic acid formationApparent molecular massChlorophyll biosynthesisGlutamyl-tRNAHomologous tRNAsSecond enzymeActive enzymeMolecular massNondenaturing conditionsDifferent chromatographic separationsCertain bacteriaReductaseDelta-aminolevulinic acidReinhardtiiPorphyrin biosynthesisBiosynthesisStable complexesChromatographic separationCarboxyl groupsEnzymatic addition of guanylate to histidine transfer RNA
Williams J, Cooley L, Söll D. Enzymatic addition of guanylate to histidine transfer RNA. Methods In Enzymology 1990, 181: 451-462. PMID: 2166216, DOI: 10.1016/0076-6879(90)81143-i.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChromatography, AffinityChromatography, DEAE-CelluloseChromatography, Ion ExchangeDrosophilaElectrophoresis, Polyacrylamide GelGuanosine TriphosphateKineticsNucleotidyltransferasesPhosphorus RadioisotopesRadioisotope Dilution TechniqueRNA, Transfer, Amino Acid-SpecificRNA, Transfer, HisSaccharomyces cerevisiaeSubstrate SpecificityConceptsHistidine tRNATransfer RNABacteriophage T5Yeast enzymeEnzyme migratesUridine residuesExtra nucleotidesLigase mechanismAdditional nucleotidesEnzymatic additionGel filtration chromatographyEnzyme intermediateTRNAAbsolute requirementEnzymeMolecular weightNucleotidesUltrogel AcA 34Filtration chromatographyATPDrosophilaAcA 34Molecular weight markersYeastTitration experiments
1986
The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA
Schön A, Krupp G, Gough S, Berry-Lowe S, Kannangara C, Söll D. The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA. Nature 1986, 322: 281-284. PMID: 3637637, DOI: 10.1038/322281a0.Peer-Reviewed Original ResearchConceptsΔ-aminolevulinatePeptide bond synthesisCognate amino acidMolecules of chlorophyllLow relative molecular massNucleotide sequence analysisRelative molecular massBond synthesisSubsequent reactionChlorophyll biosynthesisTransfer RNAUniversal precursorGlutamate tRNAAminoacyl bondSequence analysisNovel roleSerial affinity chromatographyMolecular massRNAAmino acidsComplete reactionBlue SepharoseAcceptor RNAReduction of glutamateReaction
1977
Isolation of Escherichia coli precursor tRNAs containing modified nucleoside Q.
Vögeli G, Stewart T, McCutchan T, Söll D. Isolation of Escherichia coli precursor tRNAs containing modified nucleoside Q. Journal Of Biological Chemistry 1977, 252: 2311-2318. PMID: 321455, DOI: 10.1016/s0021-9258(17)40556-4.Peer-Reviewed Original ResearchA rapid method for preparation of calf spleen exonuclease *
Silverman S, Sōll D. A rapid method for preparation of calf spleen exonuclease *. Nucleic Acids Research 1977, 4: 3511-3517. PMID: 200894, PMCID: PMC342668, DOI: 10.1093/nar/4.10.3511.Peer-Reviewed Original Research
1975
A method for the isolation of specific tRNA precursors.
Vögeli G, Grosjean H, Söll D. A method for the isolation of specific tRNA precursors. Proceedings Of The National Academy Of Sciences Of The United States Of America 1975, 72: 4790-4794. PMID: 1108001, PMCID: PMC388817, DOI: 10.1073/pnas.72.12.4790.Peer-Reviewed Original ResearchBase SequenceChromatography, AffinityEscherichia coliGlutamatesOligoribonucleotidesPancreasRibonucleasesRNA, TransferIsolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases
Marinus M, Morris N, Söll D, Kwong T. Isolation and partial characterization of three Escherichia coli mutants with altered transfer ribonucleic acid methylases. Journal Of Bacteriology 1975, 122: 257-265. PMID: 1091626, PMCID: PMC235665, DOI: 10.1128/jb.122.1.257-265.1975.Peer-Reviewed Original ResearchConceptsE. coli genomeEscherichia coli mutantsWild-type cellsEscherichia coli KAbility of RNAColi genomeColi mutantsAmber mutationGenetic lociMutagenized cellsColi KMutant strainMutantsLambda phageTRNAS-adenosylmethioninePartial characterizationGenomeCellsCrude enzymeBiosynthesisMethylasesMethylaseLociRNA