1985
[8] Glutaminyl-tRNA synthetase of Escherichia coli
Hoben P, Söll D. [8] Glutaminyl-tRNA synthetase of Escherichia coli. Methods In Enzymology 1985, 113: 55-59. PMID: 3911010, DOI: 10.1016/s0076-6879(85)13011-9.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseStructural geneSpecific aminoacyl-tRNA synthetaseE. coli chromosomeAmino acidsCognate amino acidTemperature-sensitive phenotypeGlutamyl-tRNA synthetaseAminoacyl-tRNA synthetaseColi chromosomeGln-tRNAGlnDNA fragmentsProtein synthesisEscherichia coliThermolabile enzymeCellular levelGenesGln mutationSynthetaseGlnRE. coliSeparate enzymesMultistep processNegative bacteriaEnzyme
1974
Covalent attachment of fluorescent groups to transfer ribonucleic acid. Reactions with 4-bromomethyl-7-methoxy-2-oxo-2H-benzopyran.
Yang C, Soell D. Covalent attachment of fluorescent groups to transfer ribonucleic acid. Reactions with 4-bromomethyl-7-methoxy-2-oxo-2H-benzopyran. Biochemistry 1974, 13: 3615-21. PMID: 4367729, DOI: 10.1021/bi00714a033.Peer-Reviewed Original ResearchAlkaline PhosphataseAmino Acyl-tRNA SynthetasesBenzopyransBromineCarbon RadioisotopesEscherichia coliFluorescenceFormatesGlutamatesMethionineMethyl EthersModels, ChemicalNucleosidesPhosphoric Diester HydrolasesPseudouridineRibonucleasesRNA, BacterialRNA, TransferSpectrometry, FluorescenceStructure-Activity RelationshipTransfer RNA AminoacylationUridineInvolvement of the anticodon region of Escherichia coli tRNAGln and tRNAGlu in the specific interaction with cognate aminoacyl-tRNA synthetase Alteration of the 2-thiouridine derivatives located in the anticodon of the tRNAs by BrCN or sulfur deprivation
Seno T, Agris P, Söll D. Involvement of the anticodon region of Escherichia coli tRNAGln and tRNAGlu in the specific interaction with cognate aminoacyl-tRNA synthetase Alteration of the 2-thiouridine derivatives located in the anticodon of the tRNAs by BrCN or sulfur deprivation. Biochimica Et Biophysica Acta 1974, 349: 328-338. PMID: 4366808, DOI: 10.1016/0005-2787(74)90120-8.Peer-Reviewed Original ResearchAdenosine TriphosphateAmino Acyl-tRNA SynthetasesCarbon RadioisotopesChromatography, Ion ExchangeCyanogen BromideDiphosphatesEscherichia coliGlutamatesGlutamineKineticsPhosphorus RadioisotopesProtein BiosynthesisRNA, BacterialRNA, TransferSpectrophotometry, UltravioletThiouridineTransfer RNA AminoacylationN‐(purin‐6‐ylcarbamoyl)threonine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli
Körner A, Söll D. N‐(purin‐6‐ylcarbamoyl)threonine: Biosynthesis in vitro in transfer RNA by an enzyme purified from Escherichia coli. FEBS Letters 1974, 39: 301-306. PMID: 4604806, DOI: 10.1016/0014-5793(74)80135-3.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAmino Acyl-tRNA SynthetasesCarbamatesCarbon RadioisotopesChromatography, DEAE-CelluloseChromatography, Ion ExchangeChromatography, PaperChromatography, Thin LayerElectrophoresis, PaperEscherichia coliIsotope LabelingMutationNitrosoguanidinesPhosphorus RadioisotopesPurine NucleosidesRibonucleasesRNA, BacterialRNA, TransferSpectrophotometry, UltravioletThreonineTime FactorsTritium
1973
Biological function of 2-thiouridine in Escherichia coli glutamic acid transfer ribonucleic acid.
Agris P, Soell D, Seno T. Biological function of 2-thiouridine in Escherichia coli glutamic acid transfer ribonucleic acid. Biochemistry 1973, 12: 4331-7. PMID: 4584321, DOI: 10.1021/bi00746a005.Peer-Reviewed Original ResearchAmino Acyl-tRNA SynthetasesCarbon RadioisotopesChromatography, Ion ExchangeChromatography, Thin LayerCyanogen BromideElectrophoresis, DiscElectrophoresis, Polyacrylamide GelEscherichia coliGlutamatesKineticsMutationProtein BiosynthesisRNA, BacterialRNA, TransferSpectrophotometry, UltravioletSulfurSulfur RadioisotopesThiouridineTritium