1988
Site-directed mutagenesis to fine-tune enzyme specificity
Uemura H, Rogers M, Swanson R, Watson L, Söll D. Site-directed mutagenesis to fine-tune enzyme specificity. Protein Engineering Design And Selection 1988, 2: 293-296. PMID: 3150543, DOI: 10.1093/protein/2.4.293.Peer-Reviewed Original ResearchConceptsOligonucleotide-directed mutagenesisEscherichia coli glutaminyl-tRNA synthetaseGenetic selectionGlutaminyl-tRNA synthetaseAmino acid replacementsSite-directed mutagenesisAcid replacementsEnzyme specificitySingle residueMutagenesisSide chainsRepulsive charge-charge interactionsSpecific recognitionCharge-charge interactionsNucleic acidsMutantsProteinSupFSynthetaseResiduesGlutamineSelection
1987
Allele-specific complementation of an Escherichia coli leuB mutation by a Lactobacillus bulgaricus tRNA gene
Hottinger H, Ohgi T, Zwahlen M, Dhamija S, Söll D. Allele-specific complementation of an Escherichia coli leuB mutation by a Lactobacillus bulgaricus tRNA gene. Gene 1987, 60: 75-83. PMID: 3326787, DOI: 10.1016/0378-1119(87)90215-0.Peer-Reviewed Original ResearchConceptsTRNA genesCopy numberWild-type formE. coli promotersHigh copy numberGene copy numberLeuB mutationClone bankLeucine prototrophyLow copy numberMissense suppressionNucleotide sequenceSerine tRNATerminator elementsGenesRestoration of activityTRNAEscherichia coliAmino acidsGene allelesExtra armE. coli
1985
Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene
Thorbjarnardóttir S, Dingermann T, Rafnar T, Andrésson O, Söll D, Eggertsson G. Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. Journal Of Bacteriology 1985, 161: 219-222. PMID: 2981802, PMCID: PMC214859, DOI: 10.1128/jb.161.1.219-222.1985.Peer-Reviewed Original ResearchConceptsSuppressor allelesLeuX geneAmber suppressor allelesMature coding sequenceLeucyl-tRNA synthetaseSingle base changeTRNA familiesCAA anticodonBox sequenceTermination signalDNA sequencesNucleotide sequenceBacteriophage T4Coding sequenceAminoacyl stemSuppressor geneLoop regionTRNABase changesEscherichia coliGenesE. coliSequenceColiAlleles
1969
Mechanism of protein biosynthesis.
Lengyel P, Söll D. Mechanism of protein biosynthesis. Microbiology And Molecular Biology Reviews 1969, 33: 264-301. PMID: 4896351, PMCID: PMC378322, DOI: 10.1128/br.33.2.264-301.1969.Peer-Reviewed Original Research