2006
Structure of the unusual seryl‐tRNA synthetase reveals a distinct zinc‐dependent mode of substrate recognition
Bilokapic S, Maier T, Ahel D, Gruic‐Sovulj I, Söll D, Weygand‐Durasevic I, Ban N. Structure of the unusual seryl‐tRNA synthetase reveals a distinct zinc‐dependent mode of substrate recognition. The EMBO Journal 2006, 25: 2498-2509. PMID: 16675947, PMCID: PMC1478180, DOI: 10.1038/sj.emboj.7601129.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceAnimalsArchaeal ProteinsBinding SitesCrystallography, X-RayDimerizationEnzyme ActivationHumansMethanosarcina barkeriModels, MolecularMolecular Sequence DataMolecular StructureProtein Structure, QuaternarySequence AlignmentSequence Homology, Amino AcidSerineSerine-tRNA LigaseSubstrate SpecificityThreonineConceptsSeryl-tRNA synthetaseTRNA-binding domainMinimal sequence similarityResolution crystal structureAmino acid substratesActive site zinc ionSequence similaritySubstrate recognitionSerRSsSerine substrateMotif 1Methanogenic archaeaMutational analysisProtein ligandsEnzymatic activityArchaeaAminoacyl-tRNA synthetase systemsDistinct mechanismsAbsolute requirementRecognition mechanismSynthetase systemSynthetaseIon ligandsZinc ionsEucaryotes
2004
The unusual methanogenic seryl‐tRNA synthetase recognizes tRNASer species from all three kingdoms of life
Bilokapic S, Korencic D, Söll D, Weygand‐Durasevic I. The unusual methanogenic seryl‐tRNA synthetase recognizes tRNASer species from all three kingdoms of life. The FEBS Journal 2004, 271: 694-702. PMID: 14764085, DOI: 10.1111/j.1432-1033.2003.03971.x.Peer-Reviewed Original ResearchMeSH KeywordsAnticodonBase SequenceChromatography, GelDimerizationElectrophoretic Mobility Shift AssayEscherichia coliIsoelectric FocusingMethanococcusMolecular Sequence DataNucleic Acid ConformationProtein BindingRNA, Transfer, Amino AcylRNA, Transfer, SerSerineSerine-tRNA LigaseSubstrate SpecificityTranscription, GeneticYeastsConceptsSeryl-tRNA synthetaseGel mobility shift assaysKingdoms of lifeMobility shift assaysMethanococcus jannaschiiM. maripaludisTRNA recognitionShift assaysTRNARenaturation conditionsGel filtration chromatographyConformation of tRNAComplex formationSpeciesFiltration chromatographySynthetaseDimerizationSerRSsJannaschiiTRNASerIsoacceptorsHomologuesComplementary oligonucleotidesAminoacylationRenaturation
2002
tRNA‐dependent amino acid discrimination by yeast seryl‐tRNA synthetase
Gruic‐Sovulj I, Landeka I, Söll D, Weygand‐Durasevic I. tRNA‐dependent amino acid discrimination by yeast seryl‐tRNA synthetase. The FEBS Journal 2002, 269: 5271-5279. PMID: 12392560, DOI: 10.1046/j.1432-1033.2002.03241.x.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseYeast seryl-tRNA synthetaseCognate tRNA moleculesAmino acid discriminationAminoacyl-tRNA synthetasesAmino acid substratesSimilar amino acidsAmino acid serineGenetic codeEnzyme active siteTRNA moleculesActive siteYeast SerRSConformational changesAcid substratesAmino acidsSerineSynthetaseStoichiometric analysisDifferent affinitiesEnzymeAccurate translationTRNASerSynthetasesSaccharomyces
1993
Yeast seryl‐tRNA synthetase expressed in Escherichia coli recognizes bacterial serine‐specific tRNAs in vivo
WEYGAND‐DURAŠEVIĆ I, Nenad B, Dieter J, Dieter S. Yeast seryl‐tRNA synthetase expressed in Escherichia coli recognizes bacterial serine‐specific tRNAs in vivo. The FEBS Journal 1993, 214: 869-877. PMID: 7686490, DOI: 10.1111/j.1432-1033.1993.tb17990.x.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseYeast SerRSYeast seryl-tRNA synthetaseEscherichia coliE. coli tRNAVivo complementationProkaryotic hostsTwo-step purificationSer geneHomologous tRNAsNonpermissive temperatureSer mutantE. coli strainsTRNAE. coliColi strainsColiSynthetaseSerRSVivoComplementationMutantsSaccharomycesGenesPromoter
1985
Dimeric tRNA gene arrangement in Schizosaccharomyces pombe allows increased expression of the downstream gene
Hottinger-Werlen A, Schaack J, Lapointe J, Mao J, Nichols M, Söll D. Dimeric tRNA gene arrangement in Schizosaccharomyces pombe allows increased expression of the downstream gene. Nucleic Acids Research 1985, 13: 8739-8747. PMID: 3936021, PMCID: PMC318948, DOI: 10.1093/nar/13.24.8739.Peer-Reviewed Original ResearchConceptsTRNASer geneS. pombe genesDimeric arrangementPombe geneTRNA genesGene arrangementSchizosaccharomyces pombeSpecies genesMinor genesTranscription factorsDownstream genesTranscriptional efficiencyCompetitive abilityGenesMinor speciesMajor speciesSpeciesDimeric structureEfficient productionExpressionSchizosaccharomycesPombeTRNASerSaccharomycesSequenceMutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe.
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations preventing expression of sup3 tRNASer nonsense suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. PMID: 3921825, PMCID: PMC366785, DOI: 10.1128/mcb.5.4.808.Peer-Reviewed Original ResearchConceptsTRNA genesSchizosaccharomyces pombeGenomic clone bankEucaryotic tRNA genesTranscription control regionsIdentification of mutationsClone bankTRNA precursorsControl regionNonsense codonGenetic evidenceNonsense suppressorsRevertant allelesTranscriptional efficiencySaccharomyces cerevisiae extractSequence analysisSuppressor locusColony hybridizationMutational hotspotsPoint mutationsCerevisiae extractGenesPombeMutationsSplicing
1984
Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor.
Willis I, Hottinger H, Pearson D, Chisholm V, Leupold U, Söll D. Mutations affecting excision of the intron from a eukaryotic dimeric tRNA precursor. The EMBO Journal 1984, 3: 1573-1580. PMID: 6430697, PMCID: PMC557561, DOI: 10.1002/j.1460-2075.1984.tb02013.x.Peer-Reviewed Original ResearchConceptsTRNA precursorsDimeric tRNA precursorSerine tRNA geneEfficiency of splicingPrecursor tRNA processingSingle base changeTRNA genesTRNASer geneTRNA processingGene transcriptionNucleotide sequenceUGA mutationsD-loopMutant geneGenesBase changesExtra armMutationsIntronsTranscriptionVivo systemDimeric precursorSequenceTRNASerSplicing
1982
Nonsense suppression in Schizosaccharomyces pombe: The S. pombe Sup3-e tRNASerUGA gene is active in S. cerevisiae
Hottinger H, Pearson D, Yamao F, Gamulin V, Colley L, Cooper T, Söll D. Nonsense suppression in Schizosaccharomyces pombe: The S. pombe Sup3-e tRNASerUGA gene is active in S. cerevisiae. Molecular Genetics And Genomics 1982, 188: 219-224. PMID: 6818425, DOI: 10.1007/bf00332678.Peer-Reviewed Original Research
1979
Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs.
Kohli J, Kwong T, Altruda F, Söll D, Wahl G. Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs. Journal Of Biological Chemistry 1979, 254: 1546-1551. PMID: 762155, DOI: 10.1016/s0021-9258(17)37806-7.Peer-Reviewed Original ResearchConceptsOpal suppressor tRNASuppressor tRNAReadthrough productSchizosaccharomyces pombeFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeOchre suppressor tRNAUGA termination codonBeta-globin mRNARabbit beta-globin mRNARabbit globin mRNAWheat germ extractSuppressor mutantsS. pombeNonsense suppressionPure tRNAsAlpha-globinSerine tRNATermination codonGlobin mRNATRNAPombeGerm extractSerineAssay systemThe nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe
Rafalski A, Kohli J, Agris P, Söll D. The nucleotide sequence of a UGA suppressor serine tRNA from Schizosaccharomyces pombe. Nucleic Acids Research 1979, 6: 2683-2695. PMID: 461200, PMCID: PMC327885, DOI: 10.1093/nar/6.8.2683.Peer-Reviewed Original Research
1971
Isolation and Partial Characterization of Temperature-Sensitive Escherichia coli Mutants with Altered Leucyl- and Seryl-Transfer Ribonucleic Acid Synthetases
Low B, Gates F, Goldstein T, Söll D. Isolation and Partial Characterization of Temperature-Sensitive Escherichia coli Mutants with Altered Leucyl- and Seryl-Transfer Ribonucleic Acid Synthetases. Journal Of Bacteriology 1971, 108: 742-750. PMID: 4942762, PMCID: PMC247134, DOI: 10.1128/jb.108.2.742-750.1971.Peer-Reviewed Original ResearchConceptsLeucyl-tRNA synthetaseTemperature-sensitive Escherichia coli mutantsCorresponding genetic lociEscherichia coli mutantsSeryl-tRNA synthetaseTemperature-sensitive mutantColi mutantsGenetic lociBranched-chain amino acidsEscherichia coliAmino acidsConditional growthSynthetaseMutantsPartial characterizationEnzymeA Comparative Study of the Interactions of Escherichia coli Leucyl-, Seryl-, and Valyl-Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids
Myers G, Blank H, Söll D. A Comparative Study of the Interactions of Escherichia coli Leucyl-, Seryl-, and Valyl-Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids. Journal Of Biological Chemistry 1971, 246: 4955-4964. PMID: 4936720, DOI: 10.1016/s0021-9258(18)61956-8.Peer-Reviewed Original ResearchConceptsEscherichia coli KSeryl-tRNA synthetaseLeucyl-tRNA synthetaseRibonucleic acidTransfer ribonucleic acidValyl-tRNA synthetaseTRNA recognitionColi KSynthetase-tRNA complexIsoacceptorsAmino acidsEquilibrium binding studiesPing-pong typeTRNASynthetaseEnzymeKm valuesSubstrate inhibitionBasic similaritiesBinding studiesSerylAcidATPSame bufferSequenceEnzymatic Modification of Transfer RNA
Söll D. Enzymatic Modification of Transfer RNA. Science 1971, 173: 293-299. PMID: 4934576, DOI: 10.1126/science.173.3994.293.Peer-Reviewed Original ResearchConceptsTRNA-modifying enzymesTRNA moleculesTRNA genesTransfer RNAAmino acid biosynthesisParticular tRNA speciesNucleic acid-protein interactionsAminoacyl-tRNA synthetaseSimple selection procedureAmino acid acceptorPrecursor tRNAsActive tRNAMature tRNACellular processesTRNA speciesRegulatory mutantsAcid biosynthesisRNA precursorsProtein factorsIsoacceptor tRNAsDifferent amino acidsNucleotide sequencePolynucleotide levelBiological functionsTRNA
1970
The Interaction of Seryl and of Leucyl Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids
Knowles J, Katze J, Konigsberg W, Söll D. The Interaction of Seryl and of Leucyl Transfer Ribonucleic Acid Synthetases with Their Cognate Transfer Ribonucleic Acids. Journal Of Biological Chemistry 1970, 245: 1407-1415. PMID: 4910800, DOI: 10.1016/s0021-9258(18)63251-x.Peer-Reviewed Original ResearchConceptsSeryl-tRNA synthetaseTransfer ribonucleic acidComplex formationTransfer RNA speciesLeucyl-tRNA synthetaseRibonucleic acidRNA speciesCognate tRNAEscherichia coliSynthetaseDensity gradient centrifugationTRNAStable complexesHigh saltGradient centrifugationSpeciesGel filtrationComplexesSerylColiATPEnzymeAcidSerFormationPurification of Five Serine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Homologous and Heterologous Seryl Transfer Ribonucleic Acid Synthetases
Roy K, Söll D. Purification of Five Serine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Homologous and Heterologous Seryl Transfer Ribonucleic Acid Synthetases. Journal Of Biological Chemistry 1970, 245: 1394-1400. PMID: 4910052, DOI: 10.1016/s0021-9258(18)63249-1.Peer-Reviewed Original Research
1968
On the recognition of serine transfer RNA's specific for unrelated codons by the same seryl-transfer RNA synthetase.
Sundharadas G, Katze J, Söll D, Konigsberg W, Lengyel P. On the recognition of serine transfer RNA's specific for unrelated codons by the same seryl-transfer RNA synthetase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1968, 61: 693-700. PMID: 4879401, PMCID: PMC225215, DOI: 10.1073/pnas.61.2.693.Peer-Reviewed Original ResearchStudies on polynucleotides LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression
Söll D. Studies on polynucleotides LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression. Journal Of Molecular Biology 1968, 34: 175-187. PMID: 4938541, DOI: 10.1016/0022-2836(68)90243-x.Peer-Reviewed Original ResearchConceptsSuppressor tRNAColi cellsAmber suppressor genesAmber suppressor tRNAProtein synthesis experimentsEscherichia coli cellsE. coli cellsAmber mutantsTRNASuppressor geneProtein synthesisCrude tRNAGenesRNAPartial purificationBacteriophage f2CellsMutantsRibosomesUAGSpeciesMessengerSuppressionChain terminationBindingBiosynthesis of the Peptidoglycan of Bacterial Cell Walls VII. Incorporation of Serine and Glycine into Interpeptide Bridges in Staphylococcus Epidermidis
Petit J, Strominger J, Söll D. Biosynthesis of the Peptidoglycan of Bacterial Cell Walls VII. Incorporation of Serine and Glycine into Interpeptide Bridges in Staphylococcus Epidermidis. Journal Of Biological Chemistry 1968, 243: 757-767. PMID: 5638592, DOI: 10.1016/s0021-9258(19)81730-1.Peer-Reviewed Original ResearchMeSH KeywordsAlcoholsBacitracinCarbon IsotopesCell WallChloramphenicolChlortetracyclineChromatography, Ion ExchangeCycloserineErythromycinGlycoproteinsLincomycinNovobiocinPenicillinsPeptide BiosynthesisPolysaccharides, BacterialPuromycinRistocetinSerineStaphylococcusStreptomycinSurface-Active AgentsTritiumVancomycinConceptsPeptidoglycan synthesisFractionation of Escherichia coli transfer RNA on benzoylated DEAE-cellulose
Roy K, Söll D. Fractionation of Escherichia coli transfer RNA on benzoylated DEAE-cellulose. Biochimica Et Biophysica Acta 1968, 161: 572-574. PMID: 4875424, DOI: 10.1016/0005-2787(68)90137-8.Peer-Reviewed Original Research
1966
sRNA specificity for codon recognition as studied by the ribosomal binding technique.
Söll D, Cherayil J, Jones D, Faulkner R, Hapel A, Bock R, Khorana H. sRNA specificity for codon recognition as studied by the ribosomal binding technique. Cold Spring Harbor Symposia On Quantitative Biology 1966, 31: 51-61. PMID: 4866399, DOI: 10.1101/sqb.1966.031.01.011.Peer-Reviewed Original Research