2024
Engineered mRNA–ribosome fusions for facile biosynthesis of selenoproteins
Thaenert A, Sevostyanova A, Chung C, Vargas-Rodriguez O, Melnikov S, Söll D. Engineered mRNA–ribosome fusions for facile biosynthesis of selenoproteins. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2321700121. PMID: 38442159, PMCID: PMC10945757, DOI: 10.1073/pnas.2321700121.Peer-Reviewed Original ResearchConceptsSelenocysteine insertion sequenceRibosomal RNARibosome engineeringMessenger RNARegulatory RNA elementsMachinery of protein synthesisInsertion of SecSec-containing proteinsSite-specific insertionBiosynthesis of selenoproteinsNatural messenger RNALive bacterial cellsRNA elementsUAG codonInsertion sequenceRibosome structureUGA codonSec codonInsert SecStop codonSynthetic biologyDesigning proteinsRibosomePolypeptide chainBacterial cells
2022
Uncovering translation roadblocks during the development of a synthetic tRNA
Prabhakar A, Krahn N, Zhang J, Vargas-Rodriguez O, Krupkin M, Fu Z, Acosta-Reyes FJ, Ge X, Choi J, Crnković A, Ehrenberg M, Puglisi EV, Söll D, Puglisi J. Uncovering translation roadblocks during the development of a synthetic tRNA. Nucleic Acids Research 2022, 50: 10201-10211. PMID: 35882385, PMCID: PMC9561287, DOI: 10.1093/nar/gkac576.Peer-Reviewed Original ResearchConceptsOrthogonal translation systemGenetic code expansionCode expansionTertiary interactionsNon-canonical amino acidsAminoacyl-tRNA substratesDomains of lifeAminoacyl-tRNA synthetaseTranslation systemSingle nucleotide mutationsSingle-molecule fluorescenceDistinct tRNAsNon-canonical structuresSelenocysteine insertionRibosomal translationTRNARibosomesSynthetic tRNANucleotide mutationsAmino acidsSame organismP siteOrganismsTranslocationTranslation
1971
Purification of Five Leucine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Heterologous Leucyl-Transfer Ribonucleic Acid Synthetase
Blank H, Söll D. Purification of Five Leucine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Heterologous Leucyl-Transfer Ribonucleic Acid Synthetase. Journal Of Biological Chemistry 1971, 246: 4947-4950. PMID: 4936719, DOI: 10.1016/s0021-9258(18)61954-4.Peer-Reviewed Original ResearchMeSH KeywordsAcylationBase SequenceBenzoatesCarbon IsotopesCarcinomaCell LineChromatography, DEAE-CelluloseChromatography, GelDrug StabilityEscherichia coliGenetic CodeHot TemperatureKineticsLeucineLigasesMouth NeoplasmsNucleic Acid DenaturationPolynucleotidesRibosomesRNA, BacterialRNA, TransferTemplates, GeneticYeasts
1970
Purification of Five Serine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Homologous and Heterologous Seryl Transfer Ribonucleic Acid Synthetases
Roy K, Söll D. Purification of Five Serine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Homologous and Heterologous Seryl Transfer Ribonucleic Acid Synthetases. Journal Of Biological Chemistry 1970, 245: 1394-1400. PMID: 4910052, DOI: 10.1016/s0021-9258(18)63249-1.Peer-Reviewed Original Research
1969
Mechanism of protein biosynthesis.
Lengyel P, Söll D. Mechanism of protein biosynthesis. Microbiology And Molecular Biology Reviews 1969, 33: 264-301. PMID: 4896351, PMCID: PMC378322, DOI: 10.1128/br.33.2.264-301.1969.Peer-Reviewed Original Research
1968
Structure and function of Escherichia coli ribosomes II. Translational fidelity and efficiency in protein synthesis of a protein-deficient subribosomal particle
Traub P, Söll D, Nomura M. Structure and function of Escherichia coli ribosomes II. Translational fidelity and efficiency in protein synthesis of a protein-deficient subribosomal particle. Journal Of Molecular Biology 1968, 34: 595-608. PMID: 4938559, DOI: 10.1016/0022-2836(68)90183-6.Peer-Reviewed Original ResearchStudies on polynucleotides LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression
Söll D. Studies on polynucleotides LXXXV. Partial purification of an amber supressor tRNA and studies on in vitro suppression. Journal Of Molecular Biology 1968, 34: 175-187. PMID: 4938541, DOI: 10.1016/0022-2836(68)90243-x.Peer-Reviewed Original ResearchConceptsSuppressor tRNAColi cellsAmber suppressor genesAmber suppressor tRNAProtein synthesis experimentsEscherichia coli cellsE. coli cellsAmber mutantsTRNASuppressor geneProtein synthesisCrude tRNAGenesRNAPartial purificationBacteriophage f2CellsMutantsRibosomesUAGSpeciesMessengerSuppressionChain terminationBindingBiosynthesis of the Peptidoglycan of Bacterial Cell Walls X. Further Study of the Glycyl Transfer Ribonucleic Acids Active in Peptidoglycan Synthesis in Staphylococcus Aureus
Bumsted R, Dahl J, Söll D, Strominger J. Biosynthesis of the Peptidoglycan of Bacterial Cell Walls X. Further Study of the Glycyl Transfer Ribonucleic Acids Active in Peptidoglycan Synthesis in Staphylococcus Aureus. Journal Of Biological Chemistry 1968, 243: 779-782. PMID: 4867040, DOI: 10.1016/s0021-9258(19)81733-7.Peer-Reviewed Original Research
1967
Studies on polynucleotides LXXV. Specificity of tRNA for codon recognition as studied by the ribosomal binding technique
Söll D, Cherayil J, Bock R. Studies on polynucleotides LXXV. Specificity of tRNA for codon recognition as studied by the ribosomal binding technique. Journal Of Molecular Biology 1967, 29: 97-112. PMID: 4861614, DOI: 10.1016/0022-2836(67)90183-0.Peer-Reviewed Original ResearchConceptsTransfer RNAAmino acidsE. coliIndividual amino acidsCodon recognitionMultiple codonsMultiple speciesRespective amino acidsWobble hypothesisYeast transfer RNAEscherichia coliCodonRNAColiYeastSpeciesBindingRecognition patternsTRNARibosomesThird letterStimulation of bindingTrinucleotideAcidInteresting differencesStudies on polynucleotides LXVII. Initiation of protein synthesis in vitro as studied by using ribopolynucleotides with repeating nucleotide sequences as messengers
Ghosh H, Söll D, Khorana H. Studies on polynucleotides LXVII. Initiation of protein synthesis in vitro as studied by using ribopolynucleotides with repeating nucleotide sequences as messengers. Journal Of Molecular Biology 1967, 25: 275-298. PMID: 5340533, DOI: 10.1016/0022-2836(67)90142-8.Peer-Reviewed Original ResearchAn analysis of arginine codon multiplicity in rabbit hemoglobin
Weisblum B, Cherayil J, Bock R, Söll D. An analysis of arginine codon multiplicity in rabbit hemoglobin. Journal Of Molecular Biology 1967, 28: 275-280. PMID: 4861175, DOI: 10.1016/s0022-2836(67)80009-3.Peer-Reviewed Original Research
1966
Specificity of sRNA for recognition of codons as studied by the ribosomal binding technique
Söll D, Jones D, Ohtsuka E, Faulkner R, Lohrmann R, Hayatsu H, Khorana H, Cherayil J, Hampel A, Bock R. Specificity of sRNA for recognition of codons as studied by the ribosomal binding technique. Journal Of Molecular Biology 1966, 19: 556-573. PMID: 5338858, DOI: 10.1016/s0022-2836(66)80023-2.Peer-Reviewed Original ResearchsRNA specificity for codon recognition as studied by the ribosomal binding technique.
Söll D, Cherayil J, Jones D, Faulkner R, Hapel A, Bock R, Khorana H. sRNA specificity for codon recognition as studied by the ribosomal binding technique. Cold Spring Harbor Symposia On Quantitative Biology 1966, 31: 51-61. PMID: 4866399, DOI: 10.1101/sqb.1966.031.01.011.Peer-Reviewed Original Research
1965
Studies on polynucleotides, XLIX. Stimulation of the binding of aminoacyl-sRNA's to ribosomes by ribotrinucleotides and a survey of codon assignments for 20 amino acids.
Söll D, Ohtsuka E, Jones D, Lohrmann R, Hayatsu H, Nishimura S, Khorana H. Studies on polynucleotides, XLIX. Stimulation of the binding of aminoacyl-sRNA's to ribosomes by ribotrinucleotides and a survey of codon assignments for 20 amino acids. Proceedings Of The National Academy Of Sciences Of The United States Of America 1965, 54: 1378-1385. PMID: 5325653, PMCID: PMC219908, DOI: 10.1073/pnas.54.5.1378.Peer-Reviewed Original Research