1990
Yeast suppressor mutations and transfer RNA processing
Nichols M, Willis I, Söll D. Yeast suppressor mutations and transfer RNA processing. Methods In Enzymology 1990, 181: 377-394. PMID: 2199758, DOI: 10.1016/0076-6879(90)81137-j.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBlotting, NorthernChromosomes, FungalGenes, FungalIndicators and ReagentsMolecular Sequence DataMutationNucleic Acid ConformationNucleic Acid HybridizationRNA Polymerase IIIRNA Processing, Post-TranscriptionalRNA, TransferRNA, Transfer, SerSaccharomyces cerevisiaeSuppression, GeneticTranscription FactorsTranscription, GeneticConceptsTRNA genesMature-sized tRNAsRNA processing reactionsPrimer-directed mutagenesisAminoacyl-tRNA synthetaseTransfer RNA moleculesCognate aminoacyl-tRNA synthetaseRNA processingSuppressor mutationsTRNA locusElongation factorProtein biosynthesisRibosomal interactionsRNA moleculesMutant strainStructural proteinsPink coloniesTranscription efficiencyProcessing reactionsProtein synthesisSuppressor functionTRNALow template concentrationsGenesLoci
1985
Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe
Pearson D, Willis I, Hottinger H, Bell J, Kumar A, Leupold U, Söll D. Mutations Preventing Expression of sup3 tRNASer Nonsense Suppressors of Schizosaccharomyces pombe. Molecular And Cellular Biology 1985, 5: 808-815. DOI: 10.1128/mcb.5.4.808-815.1985.Peer-Reviewed Original ResearchSup3-e geneTRNA genesInternal transcriptional control regionSuppression of nonsense codonsGenomic clone bankEucaryotic tRNA genesSaccharomyces cerevisiae extractsTranscriptional control regionIdentification of mutationsSchizosaccharomyces pombeColony hybridizationRevertant allelesTRNA precursorsClone bankNonsense codonSuppressor locusGenetic evidenceSequence analysisControl regionMutational hotspotsTranscription efficiencyPoint mutationsGenesMutationsSup3
1984
Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster
Sharp S, Garcia A, Cooley L, Söll D. Transcriptionally active and inactive gene repeats within the D. meianogaster 5S RNA gene cluster. Nucleic Acids Research 1984, 12: 7617-7632. PMID: 6093044, PMCID: PMC320189, DOI: 10.1093/nar/12.20.7617.Peer-Reviewed Original ResearchConceptsEfficiency of transcriptionRRNA gene copiesHigh transcription efficiencyTwo-nucleotide deletionD. melanogasterGene repeatRNA genesGene clusterPrimary transcriptGene copiesTranscription functionTranscription efficiencyTemplate activityCell extractsTranscriptionDNAPosition 28Position 86DNA typesRepeat unitsDeletionSame sequenceMelanogasterRRNAGenes
1983
Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions.
Dingermann T, Sharp S, Schaack J, Söll D. Stable transcription complex formation of eukaryotic tRNA genes is dependent on a limited separation of the two intragenic control regions. Journal Of Biological Chemistry 1983, 258: 10395-10402. PMID: 6309803, DOI: 10.1016/s0021-9258(17)44470-x.Peer-Reviewed Original ResearchConceptsIntragenic control regionControl regionTRNA genesTRNAArg geneDrosophila Kc cell extractStable transcription complex formationTranscription efficiencyDrosophila tRNAArg geneT-control regionMutant tRNA genesEukaryotic tRNA genesTranscription complex formationStable transcription complexesWild-type geneXhoI linkerTranscriptional roleTranscription initiationTranscription complexDNA regionsType genesTranscription factorsGene transcriptionTermination sitesDNA fragmentsCell extracts
1982
The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract.
Dingermann T, Burke D, Sharp S, Schaack J, Söll D. The 5- flanking sequences of Drosophila tRNAArg genes control their in vitro transcription in a Drosophila cell extract. Journal Of Biological Chemistry 1982, 257: 14738-14744. PMID: 6924656, DOI: 10.1016/s0021-9258(18)33342-8.Peer-Reviewed Original ResearchConceptsDrosophila tRNAArg geneTRNAArg geneDrosophila Kc cell extractFlanking sequencesTranscription efficiencyCell extractsDrosophila cell extractsEfficiency of transcriptionRegion 42ATranscriptional componentsGene clusterDrosophila extractsEfficient transcriptionTranscription factorsChromosome 2HeLa extractsTranscriptionGenesHeLa cellsSpecific sequencesHomologous extractsSequenceArgDeletionExtract