2001
Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis
Stathopoulos C, Kim W, Li T, Anderson I, Deutsch B, Palioura S, Whitman W, Söll D. Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 14292-14297. PMID: 11717392, PMCID: PMC64675, DOI: 10.1073/pnas.201540498.Peer-Reviewed Original ResearchConceptsCysteinyl-tRNA synthetaseMethanococcus maripaludisArchaeon Methanococcus maripaludisLateral gene transferNormal growth conditionsAminoacyl-tRNA synthetasesAminoacyl-tRNA synthetaseArchaea Methanocaldococcus jannaschiiProlyl-tRNA synthetaseCysS genesCys-tRNAMethanocaldococcus jannaschiiM. maripaludisSynthetase geneIntriguing enzymeMethanothermobacter thermautotrophicusCysteinyl-tRNAKnockout strainProlyl-tRNAGene transferSynthetaseBiochemical analysisVivo translationGrowth conditionsCysRS
1994
Thiobacillus ferrooxidans tyrosyl-tRNA synthetase functions in vivo in Escherichia coli
Salazar O, Sagredo B, Jedlicki E, Söll D, Weygand-Durasevic I, Orellana O. Thiobacillus ferrooxidans tyrosyl-tRNA synthetase functions in vivo in Escherichia coli. Journal Of Bacteriology 1994, 176: 4409-4415. PMID: 7517395, PMCID: PMC205654, DOI: 10.1128/jb.176.14.4409-4415.1994.Peer-Reviewed Original ResearchMeSH KeywordsAcidithiobacillus thiooxidansAmino Acid SequenceBase SequenceGene Expression Regulation, BacterialGenes, BacterialGenetic Complementation TestMolecular Sequence DataMutationNucleic Acid HybridizationOperonPromoter Regions, GeneticRNA, BacterialRNA, RibosomalRNA, Transfer, TyrSequence Analysis, DNATyrosine-tRNA LigaseConceptsOverall identityTyrosyl-tRNA synthetase geneRho-independent transcription terminatorEscherichia coli TyrRSClass I aminoacyl-tRNA synthetasesRibosomal RNA operonSingle-copy geneAminoacyl-tRNA synthetasesTyrosyl-tRNA synthetasesSouthern blot analysisRNA operonBioleaching of mineralsThermosensitive mutationTranscription unitTranscription terminatorSynthetase genePutative promoterProtein sequencesSynthetase functionE. coli strainsGenesSignature sequencesEscherichia coliAmino acidsDNA probes
1992
Organization and nucleotide sequence of the glutamine synthetase (glnA) gene from Lactobacillus delbrueckii subsp. bulgaricus
Ishino Y, Morgenthaler P, Hottinger H, Söll D. Organization and nucleotide sequence of the glutamine synthetase (glnA) gene from Lactobacillus delbrueckii subsp. bulgaricus. Applied And Environmental Microbiology 1992, 58: 3165-3169. PMID: 1359838, PMCID: PMC183065, DOI: 10.1128/aem.58.9.3165-3169.1992.Peer-Reviewed Original ResearchConceptsOpen reading frameGlnA geneReading frameGlnA gene expressionGlutamine synthetase geneL. delbrueckii subspDNA sequence analysisMaxicell methodLactobacillus delbrueckii subspDelbrueckii subspGlnR geneDeletion strainNegative eubacteriaPositive eubacteriaSynthetase geneExtensive homologyRepressor geneNucleotide sequencePresent upstreamGene expressionBamHI fragmentUnknown functionSequence analysisClostridium acetobutylicumGenes
1988
Genomic organization of tRNA and aminoacyl-tRNA synthetase genes for two amino acids in Saccharomyces cerevisiae
Kolman C, Snyder M, Söll D. Genomic organization of tRNA and aminoacyl-tRNA synthetase genes for two amino acids in Saccharomyces cerevisiae. Genomics 1988, 3: 201-206. PMID: 3066745, DOI: 10.1016/0888-7543(88)90080-8.Peer-Reviewed Original ResearchConceptsAminoacyl-tRNA synthetase genesContour-clamped homogeneous electric field gel electrophoresisHomogeneous electric field gel electrophoresisSynthetase geneGenomic organizationSmall multigene familyDNA gel blotsAmino acidsField gel electrophoresisGel electrophoresisTRNA genesChromosome assignmentMultigene familyGel blotsGene sequencesS. cerevisiaeChromosomal DNATRNAGenesSaccharomycesAspartic acidElectrophoresisGenomeCerevisiaeFamily
1984
Transfer RNA mischarging mediated by a mutant Escherichia coli glutaminyl-tRNA synthetase.
Inokuchi H, Hoben P, Yamao F, Ozeki H, Söll D. Transfer RNA mischarging mediated by a mutant Escherichia coli glutaminyl-tRNA synthetase. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 5076-5080. PMID: 6382258, PMCID: PMC391640, DOI: 10.1073/pnas.81.16.5076.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseGlnS geneEscherichia coli glutaminyl-tRNA synthetaseAminoacyl-tRNA synthetase genesEarlier genetic studiesAmber suppressor tRNAWild-type enzymeSynthetase geneTRNA speciesAmber anticodonAmber mutationMutant tRNAsSuppressor tRNAGene productsAltered specificityGln mutantMutant geneTransducing phageEnzyme structureGenetic studiesTRNAGenesMischargingBiochemical meansAminoacylation reactionIn vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene.
Cheung A, Söll D. In vivo and in vitro transcription of the Escherichia coli glutaminyl-tRNA synthetase gene. Journal Of Biological Chemistry 1984, 259: 9953-9958. PMID: 6086662, DOI: 10.1016/s0021-9258(17)42791-8.Peer-Reviewed Original ResearchConceptsGlutaminyl-tRNA synthetaseGlutaminyl-tRNA synthetase geneTranslation initiation codon AUGInitiation codon AUGPresence of tRNATermination codon UAAGlnS geneTerminator structureTranscription initiationSynthetase geneTranscription initiatesCodons UAARegulatory signalsCodon AUGTermination sitesTranscription productsSequence analysisStructure upstreamStructural regionsTranscriptionGenesTranscriptsSynthetaseMRNAGln